Antarctic pack ice Bacterial (16S) communities

最新版本 由 SCAR - Microbial Antarctic Resource System 發佈於 Mar 19, 2019 SCAR - Microbial Antarctic Resource System

Amplicon sequencing dataset of Bacterial communities (16S ssu rRNA gene) in pack ice from the Southern Ocean, near Antarctica.

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版本

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如何引用

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Eronen-Rasimus E, Luhtanen A, Rintala J, Delille B, Dieckmann G, Karkman A, Tison J (2018): Antarctic pack ice Bacterial (16S) communities. v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=antarctic_pack_ice_bacterial_communities&v=1.2

Rights

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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF 註冊

此資源已向GBIF註冊,並指定以下之GBIF UUID: bd9de0e6-69c1-4ec6-b49b-fa6f84454163。  SCAR - Microbial Antarctic Resource System 發佈此資源,並經由Scientific Committee on Antarctic Research同意向GBIF註冊成為資料發佈者。

關鍵字

Metadata

聯絡資訊

資源建立者:

Eeva Eronen-Rasimus
University of Helsink Helsink FI
Anne-Mari Luhtanen
University of Helsink Helsink FI
Janne-Markus Rintala
University of Helsink Helsink FI
Bruno Delille
Université de Liège Liège BE
Gerhard Dieckmann
Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Bremerhaven DE
Antti Karkman
University of Helsink Helsink FI
Jean-Louis Tison
Université Libre de Bruxelles Campus du Solbosch CP160/03, avenue F.D. Roosevelt 50 1050 Brussels

可回覆此資源相關問題者:

Eeva Eronen-Rasimus
University of Helsink Helsink FI

元數據填寫者:

Maxime Sweetlove
Research assistent
Royal Belgian Istitute for Natural Sciences Rue Vautier 29 1000 Brussels

與此資源的相關者:

使用者

地理涵蓋範圍

Antarctica: Southern Ocean: Wedell Sea

界定座標範圍 緯度南界 經度西界 [-67.949, -0.006], 緯度北界 經度東界 [-61.526, 0.122]

分類群涵蓋範圍

Bacteria 16S ssu rRNA marker gene, v1-v3

Domain  Bacteria (Bacteria)

時間涵蓋範圍

起始日期 / 結束日期 2013-06-21 / 2013-07-26

計畫資料

無相關描述

計畫名稱 Antarctic pack ice Bacterial (16S) communities
經費來源 The creation of this dataset was supported by the Walter and Andrée de Nottbeck Foundation, Academy of Finland and the Belgian Science Policy (Bigsouth project, SD/CA/05).

The personnel involved in the project:

Eeva Eronen-Rasimus

取樣方法

The ice cores were drilled with a trace-metal-clean (electropolished steel) ice auger, 14 cm in diameter. Two ice cores were collected and pooled at each station for the microbiological analyses. We emphasised careful sampling and subsequent sample processing to avoid contamination. The ice cores collected were cut with an ethanol-wiped handsaw into two to seven pieces, depending on the ice thickness (each horizon 10–30 cm), crushed and placed in sterile plastic containers at 4 °C over night in darkness after which the rest of the ice was quickly melted in a water bath with constant stirring. The melted samples were immediately filtered after becoming fully melted.

研究範圍 The samples were collected from 10 pack-ice stations along the Weddell and Lazarev Seas during the Antarctic Winter Ecosystem Climate Study (AWECS, leg ANT-XXIX/6)-expedition aboard the R/V Polarstern during the austral winter in June–July 2013.

方法步驟描述:

  1. For the DNA extractions, approximately 500 ml of the melted sea-ice were filtered onto sterile 0.22-μm membrane filters (Ø 47 mm; Whatman GE Healthcare, Little Chalfont, Kent, UK) and frozen in liquid nitrogen and later transferred to –80 °C.
  2. The bacterial community DNA was extracted from the filters with a PowerSoil DNA Isolation Kit (Mo Bio Laboratories Inc, Carlsbad, CA, USA), as described by Eronen-Rasimus et al. (2014), 6 months after the cruise. In addition to the samples, negative controls without the sample were extracted.
  3. The 16S ribosomal RNA gene region from V1 to part of the V3 was amplified with a polymerase chain reaction, using the universal bacterial primers F8 and R492. A two-step polymerase chain reaction and Illumina MiSeq (Illumina Inc, San Diego, CA, USA) paired-end multiplex sequencing were performed at the Institute of Biotechnology, University of Helsinki, Finland.

引用文獻

  1. Eronen-Rasimus, E., Luhtanen, A. M., Rintala, J. M., Delille, B., Dieckmann, G., Karkman, A., & Tison, J. L. (2017). An active bacterial community linked to high chl-a concentrations in Antarctic winter-pack ice and evidence for the development of an anaerobic sea-ice bacterial community. The ISME Journal, 11(10), 2345.

額外的元數據

替代的識別碼 bd9de0e6-69c1-4ec6-b49b-fa6f84454163
https://ipt.biodiversity.aq/resource?r=antarctic_pack_ice_bacterial_communities