Picoplankton (bacteria, archaea, and eukaryotic organisms < 3.0 micron) surveys conducted in collaboration with the U.S. Antarctic Marine Living Resources (AMLR) field research program aimed to study the pelagic ecosystem during month-long winter research cruises in the South Shetland Islands, Scotia Sea and the NW Weddell Sea regions in August, 2012, 2013, and 2014. This inter-annual study on the winter pelagic ecosystem follows a 25-year time series program studying austral summer waters, though is the first survey to also include the characterization of the picoplankton in this study area. The primary goal of the AMLR winter cruise was to establish the ecological importance of winter processes and plankton distributons particularly as related to sea ice and to capture the hydrography and winter distributions of krill for comparisons with summer conditions over this long times series study. The winter time study will help determine how the template for ecological success is set up for summertime production across all trophic levels. The picoplankton research will improve the understanding of winter distributions and provide sample collection opportunities for diversity and biogeochemical studies to create an integrated ecosystem picture that will be developed with the NOAA AMLR group. In particular, this effort will extend the geographic coverage of winter picoplankton in the South Shetland Islands region, both on and off the continental slope. This is important to test hypotheses concerning high latitude winter processes that were observed in coastal Antarctic Peninsula waters suggesting the relative importance of chemolithoautotrophy is a key winter-time metabolic process.
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Winter Picoplankton Diversity and Distribution in the US Antarctic Marine Living Resources Study Area - Northern Antarctic Peninsula during August and early September of 2012, 2013 and 2014. Contributed by Alison E. Murray, Carla Gimpel, Christian Reiss.
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L’éditeur et détenteur des droits de cette ressource est SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : d87b829c-43d6-4b21-afb4-37e66915c6d4. SCAR - Microbial Antarctic Resource System publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du Scientific Committee on Antarctic Research.
Bacterioplankton; picoplankton; archaea; bacterial; eukarya; Antarctic Peninsula; South Shetland Islands; Elephant Island; hydrography; photoautotrophic picoplankton
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|NCBI BioProject||http://www.ncbi.nlm.nih.gov/bioproject/316748 PRJNA316748 FASTQ|
|Antarctic Master Directory||https://gcmd.nasa.gov/search/Metadata.do?entry=d87b829c-43d6-4b21-afb4-37e66915c6d4_v1&subset=GCMD UTF-8 DIF|
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This is the U.S. Antarctic Marine Living Resources study area. Hydrographic surveys were conducted across three broad sub-areas in this region, around Elephant Island, in the Bransfield Strait, west of the South Shetland Islands.
|Enveloppe géographique||Sud Ouest [-63,5, -62,495], Nord Est [-60, -54,012]|
Bacterial, archaeal, and eukaryotic diversity in seawater picoplankton (< 3.0 micron fraction) and the diversity of the sea ice microbial community were described based on small subunit ribosomal RNA amplicon sequencing.
|Domain||Bacteria, Archaea, Eukarya|
|Date de début / Date de fin||2012-08-06 / 2012-08-14|
|Date de début / Date de fin||2013-08-14 / 2013-09-05|
|Date de début / Date de fin||2014-08-22 / 2014-09-15|
Données sur le projet
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|Titre||Assessing winter bacterioplankton distributions and carbon cycling as part of AMLR field program|
|Financement||NSF PLR 1445369|
|Description du domaine d'étude / de recherche||The study was conducted in the US AMLR Study area that covers survey regions from Elephant island to the south near Joinville Island, in Bransfield Strait, in the waters north of Elephant Island, and to the West of the South Shetland Islands. The samples described here were collected in the late winter seasons of 2012, 2013, and 2014.|
|Description du design||Hydrographic surveys were conducted at up to 110 stations per year for "fast" surveys across the 3 survey areas in this region between surface waters to a depth of 750 m (or 10 m above the bottom, if shallower). Picoplankton surveys over three years (at 6 depths between 15 - 750 m) were conducted at a total of 53 stations. Sea ice cores were also collected during 2013 on 12 occassions in a range of young (freshly produced) to multi-year ice floes.|
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Hydrographic surveys were conducted using CTD rossette outfitted with niskin bottle samplers to retrieve water samples at 15, 50, 75, 100, 200, and 750 m (or above the bottom if bottom depth was shallower than 750 m). Water was filtered immediately once on board the ship in a cold room at 0C. Samples for DNA extraction and subsequent sequencing were passed through in-line 2.7 micron filters to remove larger organisms, and the picoplankton fraction was collected on 0.2 micron Sterivex cartridge filters. A sucrose-Tris-EDTA buffer was added to the filters and they were stored frozen at -80C until extraction at our home institutions.
|Etendue de l'étude||The study was conducted in the US AMLR Study Area which covers coastal and off-shore waters in the vicinity of the South Shetland Islands and Elephant Island including the Bransifeld Strait, northern Weddell Sea, Weddell Sea confluence with the Scotia Sea, and Antarctic circumpolar current in Southern Drake Passage. We participated in three oceanographic polar winter cruises between August to early September in 2012, 2013, and 2014.|
|Contrôle qualité||Nucleic extraction blanks were carried out through amplification steps and sequence analysis quality control measures were followed on data analyzed. Oceanographic instruments were calibrated at SeaBird before the cruise and sample blanks were periodically run for chlorophyll and nutrient analyses.|
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