Winter Picoplankton Diversity and Distribution in the US Antarctic Marine Living Resources Study Area - Northern Antarctic Peninsula

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Последняя версия опубликовано SCAR - Microbial Antarctic Resource System мар. 19, 2019 SCAR - Microbial Antarctic Resource System
Дата публикации:
19 марта 2019 г.
Опубликовано:
SCAR - Microbial Antarctic Resource System
Лицензия:
CC-BY 4.0

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Описание

Picoplankton (bacteria, archaea, and eukaryotic organisms < 3.0 micron) surveys conducted in collaboration with the U.S. Antarctic Marine Living Resources (AMLR) field research program aimed to study the pelagic ecosystem during month-long winter research cruises in the South Shetland Islands, Scotia Sea and the NW Weddell Sea regions in August, 2012, 2013, and 2014. This inter-annual study on the winter pelagic ecosystem follows a 25-year time series program studying austral summer waters, though is the first survey to also include the characterization of the picoplankton in this study area. The primary goal of the AMLR winter cruise was to establish the ecological importance of winter processes and plankton distributons particularly as related to sea ice and to capture the hydrography and winter distributions of krill for comparisons with summer conditions over this long times series study. The winter time study will help determine how the template for ecological success is set up for summertime production across all trophic levels. The picoplankton research will improve the understanding of winter distributions and provide sample collection opportunities for diversity and biogeochemical studies to create an integrated ecosystem picture that will be developed with the NOAA AMLR group. In particular, this effort will extend the geographic coverage of winter picoplankton in the South Shetland Islands region, both on and off the continental slope. This is important to test hypotheses concerning high latitude winter processes that were observed in coastal Antarctic Peninsula waters suggesting the relative importance of chemolithoautotrophy is a key winter-time metabolic process.

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Winter Picoplankton Diversity and Distribution in the US Antarctic Marine Living Resources Study Area - Northern Antarctic Peninsula during August and early September of 2012, 2013 and 2014. Contributed by Alison E. Murray, Carla Gimpel, Christian Reiss.

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Публикующей организацией и владельцем прав на данную работу является SCAR - Microbial Antarctic Resource System. Эта работа находится под лицензией Creative Commons Attribution (CC-BY 4.0).

Регистрация в GBIF

Этот ресурс был зарегистрирован в GBIF, ему был присвоен следующий UUID: d87b829c-43d6-4b21-afb4-37e66915c6d4.  SCAR - Microbial Antarctic Resource System отвечает за публикацию этого ресурса, и зарегистрирован в GBIF как издатель данных при оподдержке Scientific Committee on Antarctic Research.

Ключевые слова

Bacterioplankton; picoplankton; archaea; bacterial; eukarya; Antarctic Peninsula; South Shetland Islands; Elephant Island; hydrography; photoautotrophic picoplankton

Внешние данные

Ресурс также доступен в других форматах

NCBI BioProject http://www.ncbi.nlm.nih.gov/bioproject/316748 PRJNA316748 FASTQ
Antarctic Master Directory https://gcmd.nasa.gov/search/Metadata.do?entry=d87b829c-43d6-4b21-afb4-37e66915c6d4_v1&subset=GCMD UTF-8 DIF

Контакты

Alison Murray
  • Metadata Provider ●
  • Originator ●
  • Point Of Contact ●
  • Principal Investigator
  • Research Professor
Desert Research Institute
  • 2215 Raggio Parkway
89512 Reno
NV
US
  • +1-775-673-7361
Carla Gimpel
  • Author
  • Graduate Research Student
University of Hawai'i
  • 1001 Pope Rd.
96822 Honolulu
HI
US
Christian Reiss
  • Author
  • Research Oceanographer
NOAA Fisheries, Antarctic Ecosystem Research Division
  • 8901 La Jolla Shores Dr
92037 La Jolla
CA
US

Географический охват

This is the U.S. Antarctic Marine Living Resources study area. Hydrographic surveys were conducted across three broad sub-areas in this region, around Elephant Island, in the Bransfield Strait, west of the South Shetland Islands.

Ограничивающие координаты Юг Запад [-63,5, -62,495], Север Восток [-60, -54,012]

Таксономический охват

Bacterial, archaeal, and eukaryotic diversity in seawater picoplankton (< 3.0 micron fraction) and the diversity of the sea ice microbial community were described based on small subunit ribosomal RNA amplicon sequencing.

Domain Bacteria, Archaea, Eukarya

Временной охват

Дата начала / Дата окончания 2012-08-06 / 2012-08-14
Дата начала / Дата окончания 2013-08-14 / 2013-09-05
Дата начала / Дата окончания 2014-08-22 / 2014-09-15

Данные проекта

Описание отсутсвует

Название Assessing winter bacterioplankton distributions and carbon cycling as part of AMLR field program
Финансирование NSF PLR 1445369
Описание района исследования The study was conducted in the US AMLR Study area that covers survey regions from Elephant island to the south near Joinville Island, in Bransfield Strait, in the waters north of Elephant Island, and to the West of the South Shetland Islands. The samples described here were collected in the late winter seasons of 2012, 2013, and 2014.
Описание плана выполнения исследований Hydrographic surveys were conducted at up to 110 stations per year for "fast" surveys across the 3 survey areas in this region between surface waters to a depth of 750 m (or 10 m above the bottom, if shallower). Picoplankton surveys over three years (at 6 depths between 15 - 750 m) were conducted at a total of 53 stations. Sea ice cores were also collected during 2013 on 12 occassions in a range of young (freshly produced) to multi-year ice floes.

Исполнители проекта:

Alison Murray
  • Principal Investigator

Методы сбора

Hydrographic surveys were conducted using CTD rossette outfitted with niskin bottle samplers to retrieve water samples at 15, 50, 75, 100, 200, and 750 m (or above the bottom if bottom depth was shallower than 750 m). Water was filtered immediately once on board the ship in a cold room at 0C. Samples for DNA extraction and subsequent sequencing were passed through in-line 2.7 micron filters to remove larger organisms, and the picoplankton fraction was collected on 0.2 micron Sterivex cartridge filters. A sucrose-Tris-EDTA buffer was added to the filters and they were stored frozen at -80C until extraction at our home institutions.

Охват исследования The study was conducted in the US AMLR Study Area which covers coastal and off-shore waters in the vicinity of the South Shetland Islands and Elephant Island including the Bransifeld Strait, northern Weddell Sea, Weddell Sea confluence with the Scotia Sea, and Antarctic circumpolar current in Southern Drake Passage. We participated in three oceanographic polar winter cruises between August to early September in 2012, 2013, and 2014.
Контроль качества Nucleic extraction blanks were carried out through amplification steps and sequence analysis quality control measures were followed on data analyzed. Oceanographic instruments were calibrated at SeaBird before the cruise and sample blanks were periodically run for chlorophyll and nutrient analyses.

Описание этапа методики:

  1. These details will be reported in the literature.

Дополнительные метаданные

marine, harvested by iOBIS

Альтернативные идентификаторы d87b829c-43d6-4b21-afb4-37e66915c6d4
https://ipt.biodiversity.aq/resource?r=amlr_winter_picoplankton