Bacteria on mummified seals in the Antarctic Dry Valleys

Metadatos como un archivo EML	descargar en Inglés (9 KB)
Metadatos como un archivo RTF	descargar en Inglés (11 KB)

Versiones

La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.

¿Cómo referenciar?

Los usuarios deben citar este trabajo de la siguiente manera:

Tiao G, Lee C, McDonald I, Cowan D, Cary C (2019): Bacteria on mummified seals in the Antarctic Dry Valleys. v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=antarctic_dry_valley_mummified_seal_microbiome&v=1.2

Derechos

Los usuarios deben respetar los siguientes derechos de uso:

El publicador y propietario de los derechos de este trabajo es SCAR - Microbial Antarctic Resource System. Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento (CC-BY 4.0).

Registro GBIF

Este recurso ha sido registrado en GBIF con el siguiente UUID: a3fb3af6-724d-4de3-ace8-4fa21cc729ed. SCAR - Microbial Antarctic Resource System publica este recurso y está registrado en GBIF como un publicador de datos avalado por Scientific Committee on Antarctic Research.

Palabras clave

Metadata

Contactos

Grace Tiao

Originador ●
Punto De Contacto

University of Waikato

Hamilton

NZ

Charles Lee

Originador

University of Waikato

Hamilton

NZ

Ian McDonald

Originador

University of Waikato

Hamilton

NZ

Donald Cowan

Originador

University of the Western Cape

Cape Town

ZA

Craig Cary

Originador

University of Waikato

Hamilton

NZ

Maxime Sweetlove

Proveedor De Los Metadatos

Research assistent

Royal Belgian Institute for Natural Sciences

Rue Vautier 29

1000 Brussels

msweetlove@naturalsciences.be

Cobertura geográfica

soil samples from under and around mummified crabeater seal near Lake Miers (S 78°05′36″, E 163°51′22″), Miers Valley, Victoria Land, Antarctica, that was transported to a similar site 20 m north.

Coordenadas límite	Latitud Mínima Longitud Mínima [-78,933, 163,856], Latitud Máxima Longitud Máxima [-78,933, 163,856]

Cobertura taxonómica

Amplicin sequencing of Bacteria (16S ssu rRNA gene)

Dominio	Bacteria (Bacteria)

Cobertura temporal

Fecha Inicial / Fecha Final	2005-01-15 / 2009-01-15

Datos del proyecto

No hay descripción disponible

Título	Seal Movement
Fuentes de Financiación	This work was supported through grants from the New Zealand Foundation for Research Science and Technology, Antarctica New Zealand, and the National Science Foundation (OPP-0739648 and 0944560). Additional support was provided by a George Peabody Gardner Fellowship, by the New Zealand Foundation for Research, Science and Technology Postdoctoral Research Fellowship and the New Zealand Marsden Foundation (UOW1003).

Título

Seal Movement

Fuentes de Financiación

This work was supported through grants from the New Zealand Foundation for Research Science and Technology, Antarctica New Zealand, and the National Science Foundation (OPP-0739648 and 0944560). Additional support was provided by a George Peabody Gardner Fellowship, by the New Zealand Foundation for Research, Science and Technology Postdoctoral Research Fellowship and the New Zealand Marsden Foundation (UOW1003).

Personas asociadas al proyecto:

Grace Tiao

Métodos de muestreo

Soil samples (>10 g each) were collected aseptically at a depth of 0.05m. Samples were stored in sterile plastic containers at −80 °C until analysis.

Área de Estudio	On 30 January 2006, a mummified crabeater seal was carried from its original resting site near Lake Miers (S 78°05′36″, E 163°51′22″), Miers Valley, Victoria Land, Antarctica, to a new site of similar geomorphology 20 m north. Samples for amplicon sequencing were taken from the original site before transportation (austral summer 2005), the new site before transportation (control, austral summer 2006), and the new site after incubation (austral summer 2009)

Área de Estudio

On 30 January 2006, a mummified crabeater seal was carried from its original resting site near Lake Miers (S 78°05′36″, E 163°51′22″), Miers Valley, Victoria Land, Antarctica, to a new site of similar geomorphology 20 m north. Samples for amplicon sequencing were taken from the original site before transportation (austral summer 2005), the new site before transportation (control, austral summer 2006), and the new site after incubation (austral summer 2009)

Descripción de la metodología paso a paso:

16S rRNA gene were generated using the primer pair Tx9 (5′-GGATTAGAWACCCBGGTAGTC-3′) and 1391R (5′-GACGGGCRGTGWGTRCA-3′). PCR was performed in triplicate and pooled to reduce stochastic variability between reactions. Each 30 μl reaction contained 5 or 20 ng of extracted community DNA, Pfx polymerase and platinum polymerase (0.5 U each; Invitrogen), 1× Pfx PCR buffer with Pfx enhancer, 0.2 mM dNTPs, 1 mM MgCl2, 0.02 mg ml−1 BSA, 0.8 μM of forward and reverse primer, and PCR-grade water. Thermal cycling conditions were 94 °C for 2 min; 18 or 24 cycles of 94 °C for 15 s, 55 °C for 30 s and 68 °C for 1 min; and 68 °C for 3 min.
Amplicons were size-selected and purified using polyacrylamide gel electrophoresis before being prepared for pyrosequencing by Taxon Biosciences (Tiburon, CA, USA).

Referencias bibliográficas

Tiao, G., Lee, C. K., McDonald, I. R., Cowan, D. A., & Cary, S. C. (2012). Rapid microbial response to the presence of an ancient relic in the Antarctic Dry Valleys. Nature Communications, 3, 660.

Metadatos adicionales

Identificadores alternativos	a3fb3af6-724d-4de3-ace8-4fa21cc729ed
	https://ipt.biodiversity.aq/resource?r=antarctic_dry_valley_mummified_seal_microbiome