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Antarctic Peninsula Bacterioplankton 16S rRNA gene surveys and metagenomes from Winter 2002 and Summer 2006.

Última versión Publicado por SCAR - Microbial Antarctic Resource System en 19 de marzo de 2019 SCAR - Microbial Antarctic Resource System
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Fecha de publicación:
19 de marzo de 2019
Licencia:
CC-BY 4.0

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Descripción

Antarctic surface oceans are well-studied during summer when irradiance levels are high, sea ice is melting and primary productivity is at a maximum. Coincident with this timing, the bacterioplankton respond with significant increases in secondary productivity. Little is known about bacterioplankton in winter when darkness and sea-ice cover inhibit photoautotrophic primary production. We report here an environmental genomic and small subunit ribosomal RNA (SSU rRNA) analysis of winter and summer Antarctic Peninsula coastal seawater bacterioplankton. Intense inter-seasonal differences were reflected through shifts in community composition and functional capacities encoded in winter and summer environmental genomes with significantly higher phylogenetic and functional diversity in winter. In general, inferred metabolisms of summer bacterioplankton were characterized by chemoheterotrophy, photoheterotrophy and aerobic anoxygenic photosynthesis while the winter community included the capacity for bacterial and archaeal chemolithoautotrophy. Chemolithoautotrophic pathways were dominant in winter and were similar to those recently reported in global‘dark ocean’ mesopelagic waters. If chemolithoautotrophy is widespread in the Southern Ocean in winter, this process may be a previously unaccounted carbon sink and may help account for the unexplained anomalies in surface inorganic nitrogen content.

Versiones

La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.

¿Cómo referenciar?

Los usuarios deben citar este trabajo de la siguiente manera:

Murray, AE and JJ Grzymski. 2007. Diversity and genomics of Antarctic marine microorganisms. Phil. Trans. Roy. Soc. Ser. B. 362:2259-2271.

Derechos

Los usuarios deben respetar los siguientes derechos de uso:

El publicador y propietario de los derechos de este trabajo es SCAR - Microbial Antarctic Resource System. Este trabajo está autorizado bajo una Licencia Creative Commons Atribución/Reconocimiento 4.0 Internacional (CC-BY) 4.0.

Registro GBIF

Este recurso ha sido registrado en GBIF con el siguiente UUID: 2cc6227c-a416-4928-bd1f-8458741b8377.  SCAR - Microbial Antarctic Resource System publica este recurso y está registrado en GBIF como un publicador de datos avalado por Scientific Committee on Antarctic Research.

Palabras clave

Bacterioplankton; Antarctic Peninsula; 16S rRNA; metagenome; marine; archaea; bacteria; summer; winter; Other

Contactos

¿Quién creó el recurso?:
-

Alison Murray
Associate Research Professor
DRI
2215 Raggio Parkway
89512 Reno
NV
US
775 673 7361
http://www.dri.edu/alison-murray

¿Quién puede resolver dudas acerca del recurso?:

Alison Murray
Associate Research Professor
DRI
2215 Raggio Parkway
89512 Reno
NV
US
775 673 7361
http://www.dri.edu/alison-murray

¿Quién documentó los metadatos?:
-

Alison Murray
Associate Research Professor
DRI
2215 Raggio Parkway
89512 Reno
NV
US
775 673 7361
http://www.dri.edu/alison-murray

¿Quién más está asociado con el recurso?:

Alison Murray
Investigador Principal
Associate Research Professor
DRI
2215 Raggio Parkway
89512 Reno
NV
US
775 673 7361
http://www.dri.edu/alison-murray
Joseph Grzymski
Investigador Principal
Assistant Research Professor
DRI
2215 Raggio Parkway
89512 Reno
NV
US
775 673 7478
http://www.dri.edu/joe-grzymski

Cobertura geográfica

Samples were collected in the nearshore region of Anvers Island, near Palmer Station.

Coordenadas límite Latitud Mínima Longitud Mínima [-64,78, -64,07], Latitud Máxima Longitud Máxima [-64,77, -64,05]

Cobertura taxonómica

Plankton surveys of community structure were conducted of those organisms passing through a 1.6 micron glass fiber filter.

Dominio Archaea, Bacteria

Cobertura temporal

Fecha Inicial 2002-08-20
Fecha Inicial 2006-02-28
Fecha Inicial 2002-01-17
Fecha Inicial 2002-07-17

Datos del proyecto

No hay descripción disponible

Título IPY Antarctic Peninsula Bacterioplankton
Fuentes de Financiación NSF ANT 0632278 supported the researchers and Antarctic expeditions and DOE JGI Community Sequencing Program supported the metagenome and Sanger sequencing of 16S rRNA gene libraries; Pyrosequencing was provided by the International Census of Marine Microbes (ICoMM) with financial support from a W. M. Keck foundation award to the Marine Biological Laboratory in Woods Hole.
Descripción del área de estudio Seawater (1.6 - 0.2 micron fraction) sampled off of Anvers Island, Antarctic Peninsula.
Descripción del diseño Samples were collected from surface waters on 12 occasions over the annual cycle in 2002 and in late February in 2006. The samples from 2002 were all analyzed by PCR-DGGE (Murray and Grzymski, 2007) and 3 samples were selected for Roche 454 tag sequencing targeting bacteria. In addition, the sample from 20 August 2002 was selected for (i) 16S rRNA gene clone library sequencing for bacteria and archaea; and (ii) for metagenome sequencing in which a large insert (40 kb) library was prepared and end sequences were determined for ~ 20K clones. Selected clones (~ 96) were then fully sequenced. In 2006 samples were collected at the end of February, in which we selected a sample from 28 February 2006 for 16S rRNA gene clone library sequencing for bacteria and archaea; in addition a metagenome sequencing effort was conducted in which a large insert (40 kb) library was prepared and end sequences were determined for ~ 20K clones. Selected clones (~ 96) were then fully sequenced.

Personas asociadas al proyecto:

Alison Murray
Investigador Principal

Métodos de muestreo

Seawater was collected by submersible pump and filtered at the Station, see Grzymski et al. 2012 for details.

Área de Estudio See Geographic coverage
Control de Calidad Sanger sequence data was automatically assembled and chimera checked; metagenome sequence data was automatically annotated at the Joint Genome Institute (see Grzymski et al. 2012).

Descripción de la metodología paso a paso:

  1. A MICROBIAL_SEQUENCE_SET Description file describing 9 data sets was uploaded to the IPT. 9 MIMARKS data files were uploaded to the IPT.

Referencias bibliográficas

  1. Grzymski, JJ, CS Riesenfeld , TJ Williams, AM Dussaq, H Ducklow, M Erickson, R Cavicchioli, & AE Murray. 2012. A metagenomic assessment of winter and summer bacterioplankton from Antarctic Peninsula coastal surface waters. ISME Journal. DOI:10.1038/ismej.2012.31
  2. Ghiglione, JF, and AE Murray. 2012. Pronounced summer to winter differences and higher wintertime richness in coastal Antarctic marine bacterioplankton. Environ. Microbiol. 14(3): 617-629. DOI:10.1111/j.1462-2920.2011.02601.x
  3. Williams, TJ, E Long, F Evans, MZ DeMaere, FM Lauro, MJ Raftery, H Ducklow, JJ Grzymski, AE Murray, R Cavicchioli. 2012. A metaproteomic assessment of summer and winter bacterioplankton from Antarctic Peninsula coastal surface waters. ISME Journal 6:1883-1900. doi: 10.1038/ismej.2012.28