Bacterioplankton in surface waters off the northern tip of the Antarctic Peninsula

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Última versión publicado por SCAR - Microbial Antarctic Resource System el jun 10, 2021 SCAR - Microbial Antarctic Resource System
Fecha de publicación:
10 de junio de 2021
Licencia:
CC-BY 4.0

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Descripción

Amplicon sequencing dataset (454 LS) targeting planktonic Bacteria (16S ssu rRNA) in surface layer sea water samples (n=18) from the Northern tip of the Antarctic Peninsula.

Versiones

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¿Cómo referenciar?

Los usuarios deben citar este trabajo de la siguiente manera:

Cao S, He J, Zhang F, Lin L, Gao Y, Zhou Q (2021): Bacterioplankton in surface waters off the northern tip of the Antarctic Peninsula. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=bacterioplankton_from_northern_tip_antarctic_peninsula&v=1.0

Derechos

Los usuarios deben respetar los siguientes derechos de uso:

El publicador y propietario de los derechos de este trabajo es SCAR - Microbial Antarctic Resource System. Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento (CC-BY 4.0).

Registro GBIF

Este recurso ha sido registrado en GBIF con el siguiente UUID: 6acade15-6d79-4dad-824e-a70b73db623c.  SCAR - Microbial Antarctic Resource System publica este recurso y está registrado en GBIF como un publicador de datos avalado por Scientific Committee on Antarctic Research.

Palabras clave

Metadata

Contactos

Shunan Cao
  • Originador
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN
Jianfeng He
  • Originador ●
  • Usuario ●
  • Punto De Contacto
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN
Fang Zhang
  • Originador
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN
Ling Lin
  • Originador
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN
Yuan Gao
  • Originador
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN
Qiming Zhou
  • Originador
School of Life Science and Technology, Harbin Institute of Technology
Harbin
CN
Maxime Sweetlove
  • Proveedor De Los Metadatos
Royal Belgian Institute of Natural Sciences
Brussels
BE

Cobertura geográfica

Northern tip of the Antarctic Peninsula

Coordenadas límite Latitud Mínima Longitud Mínima [-65,613, -66,441], Latitud Máxima Longitud Máxima [-64,815, -62,593]

Cobertura temporal

Fecha Inicial / Fecha Final 2011-12-30 / 2012-01-29

Datos del proyecto

No hay descripción disponible

Título CHINARE-2011–2015
Fuentes de Financiación This work was funded by the Chinese Polar Environment Comprehensive Investigation & Assessment Programs (CHINARE-2011–2015), an international cooperation programme of the Chinese National Arctic and Antarctic Research Expedition (IC201514) and the National Natural Science Foundation of China (grant nos. 41206189 and 41476168).
Descripción del área de estudio Seawater from the northern tip of the Antarctic Peninsula.

Personas asociadas al proyecto:

Jianfeng He

Métodos de muestreo

Thirteen samples were taken at 25 m depth (the layer of maximum oxygen), three samples were taken at 2 m and two samples were taken at 50 m depth. An SBE 911 plus CTD instrument combined with an SBE 32 Carousel water sampler (both by Sea-Bird Electronics) equipped with 24 Niskin bottles was used to collect seawater and measure physical parameters (temperature and salinity). +- 2 L of seawater was pre-filtered through 3 μm pore size polycarbonate membranes (Whatman), and then filtered with a vacuum pump through polycarbonate membranes (47 mm diameter, 0.2-μm pore size, Whatman). Samples were subsequently frozen at −80°C.

Área de Estudio Samples (n=18) were collected in the waters by the northern tip of the Antarctic Peninsula, including the northern area of the Bransfield Strait, the Powell Basin and the South Orkney tableland area during the 28th Chinese National Antarctic Research Expedition, from December 2011 to January 2012.
Control de Calidad The PCR products were purified using AxyPrepTM DNA Purification Kit (Axygen®) and quantified using a Qubit® 2.0 Fluorometer (Life Technologies).

Descripción de la metodología paso a paso:

  1. DNA was extracted using a modified cetyltrimethylammonium bromide method and examined by agarose gel electrophoresis.
  2. The V1–V3 region of the 16S rRNA gene of Bacteria was amplified using the universal primer pair F8 (5′-CCTATCCCCTGTGT- GCCTTGGCAGTCTCAG-AGAGTTTGATCCTGGCTCAG-3′) and R533 (5′-CCATCTCATCCCTGC- GTGTCTCCGACTCAG-NNNNNNNN-TTACCGCGGCTGCT- GGCAC-3′; NNNNNNNN being the place of the sample-specific barcode). PCR was performed using 5–10 ng genomic DNA in a final volume of 50 μL. The PCR procedure was as follows: initial denaturation at 95°C for 2 min; 25 cycles at 95°C for 30 s, 56.4°C for 1 min and 72°C for 30 s; and a final extension at 72°C for 5 min.
  3. 454 pyrosequencing was performed on an FLX Titanium Genome Sequencer (454/Roche Life Sciences).

Referencias bibliográficas

  1. Cao, S., He, J., Zhang, F., Lin, L., Gao, Y., & Zhou, Q. (2019). Diversity and community structure of bacterioplankton in surface waters off the northern tip of the Antarctic Peninsula. Polar Research.