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Bacterioplankton in surface waters off the northern tip of the Antarctic Peninsula

最新バージョン SCAR - Microbial Antarctic Resource System によって公開 2021/06/10 SCAR - Microbial Antarctic Resource System
Amplicon sequencing dataset (454 LS) targeting planktonic Bacteria (16S ssu rRNA) in surface layer sea water samples (n=18) from the Northern tip of the Antarctic Peninsula.

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バージョン

次の表は、公にアクセス可能な公開バージョンのリソースのみ表示しています。

引用方法

研究者はこの研究内容を以下のように引用する必要があります。:

Cao S, He J, Zhang F, Lin L, Gao Y, Zhou Q (2021): Bacterioplankton in surface waters off the northern tip of the Antarctic Peninsula. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=bacterioplankton_from_northern_tip_antarctic_peninsula&v=1.0

権利

研究者は権利に関する下記ステートメントを尊重する必要があります。:

パブリッシャーとライセンス保持者権利者は SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF登録

このリソースをはGBIF と登録されており GBIF UUID: 6acade15-6d79-4dad-824e-a70b73db623cが割り当てられています。   Scientific Committee on Antarctic Research によって承認されたデータ パブリッシャーとして GBIF に登録されているSCAR - Microbial Antarctic Resource System が、このリソースをパブリッシュしました。

キーワード

Metadata

連絡先

リソースを作成した人:

Shunan Cao
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN
Jianfeng He
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN
Fang Zhang
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN
Ling Lin
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN
Yuan Gao
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN
Qiming Zhou
School of Life Science and Technology, Harbin Institute of Technology
Harbin
CN

リソースに関する質問に答えることができる人:

Jianfeng He
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN

メタデータを記載した人:

Maxime Sweetlove
Royal Belgian Institute of Natural Sciences
Brussels
BE

他に、リソースに関連付けられていた人:

データ利用者
Jianfeng He
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN

地理的範囲

Northern tip of the Antarctic Peninsula

座標(緯度経度) 南 西 [-65.613, -66.441], 北 東 [-64.815, -62.593]

時間的範囲

開始日 / 終了日 2011-12-30 / 2012-01-29

プロジェクトデータ

説明がありません

タイトル CHINARE-2011–2015
ファンデイング This work was funded by the Chinese Polar Environment Comprehensive Investigation & Assessment Programs (CHINARE-2011–2015), an international cooperation programme of the Chinese National Arctic and Antarctic Research Expedition (IC201514) and the National Natural Science Foundation of China (grant nos. 41206189 and 41476168).
Study Area Description Seawater from the northern tip of the Antarctic Peninsula.

プロジェクトに携わる要員:

Jianfeng He

収集方法

Thirteen samples were taken at 25 m depth (the layer of maximum oxygen), three samples were taken at 2 m and two samples were taken at 50 m depth. An SBE 911 plus CTD instrument combined with an SBE 32 Carousel water sampler (both by Sea-Bird Electronics) equipped with 24 Niskin bottles was used to collect seawater and measure physical parameters (temperature and salinity). +- 2 L of seawater was pre-filtered through 3 μm pore size polycarbonate membranes (Whatman), and then filtered with a vacuum pump through polycarbonate membranes (47 mm diameter, 0.2-μm pore size, Whatman). Samples were subsequently frozen at −80°C.

Study Extent Samples (n=18) were collected in the waters by the northern tip of the Antarctic Peninsula, including the northern area of the Bransfield Strait, the Powell Basin and the South Orkney tableland area during the 28th Chinese National Antarctic Research Expedition, from December 2011 to January 2012.
Quality Control The PCR products were purified using AxyPrepTM DNA Purification Kit (Axygen®) and quantified using a Qubit® 2.0 Fluorometer (Life Technologies).

Method step description:

  1. DNA was extracted using a modified cetyltrimethylammonium bromide method and examined by agarose gel electrophoresis.
  2. The V1–V3 region of the 16S rRNA gene of Bacteria was amplified using the universal primer pair F8 (5′-CCTATCCCCTGTGT- GCCTTGGCAGTCTCAG-AGAGTTTGATCCTGGCTCAG-3′) and R533 (5′-CCATCTCATCCCTGC- GTGTCTCCGACTCAG-NNNNNNNN-TTACCGCGGCTGCT- GGCAC-3′; NNNNNNNN being the place of the sample-specific barcode). PCR was performed using 5–10 ng genomic DNA in a final volume of 50 μL. The PCR procedure was as follows: initial denaturation at 95°C for 2 min; 25 cycles at 95°C for 30 s, 56.4°C for 1 min and 72°C for 30 s; and a final extension at 72°C for 5 min.
  3. 454 pyrosequencing was performed on an FLX Titanium Genome Sequencer (454/Roche Life Sciences).

書誌情報の引用

  1. Cao, S., He, J., Zhang, F., Lin, L., Gao, Y., & Zhou, Q. (2019). Diversity and community structure of bacterioplankton in surface waters off the northern tip of the Antarctic Peninsula. Polar Research.