Cao S, He J, Zhang F, Lin L, Gao Y, Zhou Q (2021): Bacterioplankton in surface waters off the northern tip of the Antarctic Peninsula. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=bacterioplankton_from_northern_tip_antarctic_peninsula&v=1.0
此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
Northern tip of the Antarctic Peninsula
|界定座標範圍||緯度南界 經度西界 [-65.613, -66.441], 緯度北界 經度東界 [-64.815, -62.593]|
|起始日期 / 結束日期||2011-12-30 / 2012-01-29|
|經費來源||This work was funded by the Chinese Polar Environment Comprehensive Investigation & Assessment Programs (CHINARE-2011–2015), an international cooperation programme of the Chinese National Arctic and Antarctic Research Expedition (IC201514) and the National Natural Science Foundation of China (grant nos. 41206189 and 41476168).|
|研究區域描述||Seawater from the northern tip of the Antarctic Peninsula.|
Thirteen samples were taken at 25 m depth (the layer of maximum oxygen), three samples were taken at 2 m and two samples were taken at 50 m depth. An SBE 911 plus CTD instrument combined with an SBE 32 Carousel water sampler (both by Sea-Bird Electronics) equipped with 24 Niskin bottles was used to collect seawater and measure physical parameters (temperature and salinity). +- 2 L of seawater was pre-filtered through 3 μm pore size polycarbonate membranes (Whatman), and then filtered with a vacuum pump through polycarbonate membranes (47 mm diameter, 0.2-μm pore size, Whatman). Samples were subsequently frozen at −80°C.
|研究範圍||Samples (n=18) were collected in the waters by the northern tip of the Antarctic Peninsula, including the northern area of the Bransfield Strait, the Powell Basin and the South Orkney tableland area during the 28th Chinese National Antarctic Research Expedition, from December 2011 to January 2012.|
|品質控管||The PCR products were purified using AxyPrepTM DNA Purification Kit (Axygen®) and quantified using a Qubit® 2.0 Fluorometer (Life Technologies).|
- DNA was extracted using a modified cetyltrimethylammonium bromide method and examined by agarose gel electrophoresis.
- The V1–V3 region of the 16S rRNA gene of Bacteria was amplified using the universal primer pair F8 (5′-CCTATCCCCTGTGT- GCCTTGGCAGTCTCAG-AGAGTTTGATCCTGGCTCAG-3′) and R533 (5′-CCATCTCATCCCTGC- GTGTCTCCGACTCAG-NNNNNNNN-TTACCGCGGCTGCT- GGCAC-3′; NNNNNNNN being the place of the sample-specific barcode). PCR was performed using 5–10 ng genomic DNA in a final volume of 50 μL. The PCR procedure was as follows: initial denaturation at 95°C for 2 min; 25 cycles at 95°C for 30 s, 56.4°C for 1 min and 72°C for 30 s; and a final extension at 72°C for 5 min.
- 454 pyrosequencing was performed on an FLX Titanium Genome Sequencer (454/Roche Life Sciences).
- Cao, S., He, J., Zhang, F., Lin, L., Gao, Y., & Zhou, Q. (2019). Diversity and community structure of bacterioplankton in surface waters off the northern tip of the Antarctic Peninsula. Polar Research.