說明
Amplicon sequencing dataset of Bacteria (16S ssu rRNA gene) and Eukaryotes (18S ssu rRNA gene) from wet and dry soil samples near Miers stream, Nostoc pond, Miers lake and Buddha lake, in the McMurdo Dry Valleys, Antarctica.
版本
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如何引用
研究者應依照以下指示引用此資源。:
Niederberger T, Sohm J, Gunderson T, Parker A, Tirindelli J, Capone D, Carpenter E, Cary S (2019): Microbial communities (Eukaryotes and Bacteria) of transiently wetted vs. arid Antarctic Dry Valley soils. v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=eukaryotes_bacteria_in_wetted_vs_arid_antarctic_dry_valley_soils&v=1.2
權利
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此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: 55ed602c-dc49-41d2-a860-4a626ba85ba8。 SCAR - Microbial Antarctic Resource System 發佈此資源，並經由Scientific Committee on Antarctic Research同意向GBIF註冊成為資料發佈者。
關鍵字
Metadata
聯絡資訊
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- 元數據提供者
- Research assistent
- Rue Vautier 29
地理涵蓋範圍
Sample pairs were taken from wet and dry soil near Miers stream, Nostoc pond, Miers lake and Buddha lake, in the McMurdo Dry Valleys, Antarctica.
| 界定座標範圍 | 緯度南界 經度西界 [-78.096, 163.747], 緯度北界 經度東界 [-78.06, 163.832] |
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分類群涵蓋範圍
Eukaryote (18S ssu rRNA marker gene) and Bacterial (16S ssu rRNA marker gene) microorganisms
| Domain | Eukaryota (Eukaryotes), Bacteria (Bacteria) |
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時間涵蓋範圍
| 起始日期 / 結束日期 | 2009-01-18 / 2009-12-08 |
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計畫資料
無相關描述
| 計畫名稱 | Microbial communities (Eukaryotes and Bacteria) of transiently wetted vs. arid Antarctic Dry Valley soils |
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| 經費來源 | This research was supported by National Science Foundation Grants ANT 0739633, ANT 0739640, and ANT 0739648 and 0944560. |
參與計畫的人員:
取樣方法
Soil samples were collected aseptically at each site into sterile Whirl-Pak bags using a sterile metal spatula and kept frozen during transport to the laboratory.
| 研究範圍 | Samples were collected from the Miers Valley region of Antarctica during the summer months, including: MS1, Miers Stream 1 (S78°05.748′, E163°44.822′), a site located at the edge of the runoff stream flowing from Miers Glacier into Miers Lake in Miers Valley; ML1, Miers Lake 1 (S78°05.615′, E163°49.912′) located on the north side of Miers Lake; BL, Baby Buddha Lake (S78°03.621′, E163°46.463′), and NP, Nostoc Pond (S78°03.920′, E163°46.497′) adjacent to lake systems located over the north ridge of Miers Valley. |
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方法步驟描述:
- DNA was isolated from homogenized soil samples using the PowersoilTM DNA isolation kit (MOBIO) as per manufacturer’s instructions with all terminal-restriction fragment length polymorphisms (T-RFLPs) based on pooled triplicate PCR assays.
- Tagged amplicon pyrosequencing of the V1 to V3 region of the 16S and 18S rRNA gene (primer pairs 28F-519R and euk7F-euk570R, respectively) was performed on DNA extracts by the Research and Testing Laboratories located in Lubbock, TX (www.researchandtesting.com) using FLX technology (Roche).
引用文獻
- Niederberger, T. D., Sohm, J. A., Gunderson, T. E., Parker, A. E., Tirindelli, J., Capone, D. G., ... & Cary, S. C. (2015). Microbial community composition of transiently wetted Antarctic Dry Valley soils. Frontiers in microbiology, 6, 9.
- Niederberger, T. D., Sohm, J. A., Tirindelli, J., Gunderson, T., Capone, D. G., Carpenter, E. J., & Cary, S. C. (2012). Diverse and highly active diazotrophic assemblages inhabit ephemerally wetted soils of the Antarctic Dry Valleys. FEMS microbiology ecology, 82(2), 376-390.
額外的詮釋資料
| 替代的識別碼 | 55ed602c-dc49-41d2-a860-4a626ba85ba8 |
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| https://ipt.biodiversity.aq/resource?r=eukaryotes_bacteria_in_wetted_vs_arid_antarctic_dry_valley_soils |