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Niederberger T, Sohm J, Gunderson T, Parker A, Tirindelli J, Capone D, Carpenter E, Cary S (2019): Microbial communities (Eukaryotes and Bacteria) of transiently wetted vs. arid Antarctic Dry Valley soils. v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=eukaryotes_bacteria_in_wetted_vs_arid_antarctic_dry_valley_soils&v=1.2
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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
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This resource has been registered with GBIF, and assigned the following GBIF UUID: 55ed602c-dc49-41d2-a860-4a626ba85ba8. SCAR - Microbial Antarctic Resource System publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Scientific Committee on Antarctic Research.
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Geographic Coverage
Sample pairs were taken from wet and dry soil near Miers stream, Nostoc pond, Miers lake and Buddha lake, in the McMurdo Dry Valleys, Antarctica.
Bounding Coordinates | South West [-78.096, 163.747], North East [-78.06, 163.832] |
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Taxonomic Coverage
Eukaryote (18S ssu rRNA marker gene) and Bacterial (16S ssu rRNA marker gene) microorganisms
Domain | Eukaryota (Eukaryotes), Bacteria (Bacteria) |
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Temporal Coverage
Start Date / End Date | 2009-01-18 / 2009-12-08 |
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Project Data
No Description available
Title | Microbial communities (Eukaryotes and Bacteria) of transiently wetted vs. arid Antarctic Dry Valley soils |
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Funding | This research was supported by National Science Foundation Grants ANT 0739633, ANT 0739640, and ANT 0739648 and 0944560. |
The personnel involved in the project:
Sampling Methods
Soil samples were collected aseptically at each site into sterile Whirl-Pak bags using a sterile metal spatula and kept frozen during transport to the laboratory.
Study Extent | Samples were collected from the Miers Valley region of Antarctica during the summer months, including: MS1, Miers Stream 1 (S78°05.748′, E163°44.822′), a site located at the edge of the runoff stream flowing from Miers Glacier into Miers Lake in Miers Valley; ML1, Miers Lake 1 (S78°05.615′, E163°49.912′) located on the north side of Miers Lake; BL, Baby Buddha Lake (S78°03.621′, E163°46.463′), and NP, Nostoc Pond (S78°03.920′, E163°46.497′) adjacent to lake systems located over the north ridge of Miers Valley. |
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Method step description:
- DNA was isolated from homogenized soil samples using the PowersoilTM DNA isolation kit (MOBIO) as per manufacturer’s instructions with all terminal-restriction fragment length polymorphisms (T-RFLPs) based on pooled triplicate PCR assays.
- Tagged amplicon pyrosequencing of the V1 to V3 region of the 16S and 18S rRNA gene (primer pairs 28F-519R and euk7F-euk570R, respectively) was performed on DNA extracts by the Research and Testing Laboratories located in Lubbock, TX (www.researchandtesting.com) using FLX technology (Roche).
Bibliographic Citations
- Niederberger, T. D., Sohm, J. A., Gunderson, T. E., Parker, A. E., Tirindelli, J., Capone, D. G., ... & Cary, S. C. (2015). Microbial community composition of transiently wetted Antarctic Dry Valley soils. Frontiers in microbiology, 6, 9.
- Niederberger, T. D., Sohm, J. A., Tirindelli, J., Gunderson, T., Capone, D. G., Carpenter, E. J., & Cary, S. C. (2012). Diverse and highly active diazotrophic assemblages inhabit ephemerally wetted soils of the Antarctic Dry Valleys. FEMS microbiology ecology, 82(2), 376-390.
Additional Metadata
Alternative Identifiers | 55ed602c-dc49-41d2-a860-4a626ba85ba8 |
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https://ipt.biodiversity.aq/resource?r=eukaryotes_bacteria_in_wetted_vs_arid_antarctic_dry_valley_soils |