microbial Eukaryotes in lakes along an Argentinian-Antarctic geographical gradient

Somente metadatos
Versão mais recente published by SCAR - Microbial Antarctic Resource System on mar 20, 2019 SCAR - Microbial Antarctic Resource System
Publication date:
20 de março de 2019
Licença:
CC-BY 4.0

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Descrição

Amplicon sequencing dataset (Illumina HiSeq) of Eukaryotes (18S) in lakes along a latitudinal gradient from Southern Argentina to maritime Antarctica

Versões

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Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Schiaffino R, Lara E, Fernandez L, Balagué V, Singer D, Seppey C, Massana R, Izaguirre I (2019): microbial Eukaryotes in lakes along an Argentinian-Antarctic geographical gradient. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbial_eukaryotes_in_antarctic_and_argentinian_lakes&v=1.0

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: bc44c279-bfe6-4827-80b4-06dcb9aa7908.  SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.

Palavras-chave

Metadata

Contatos

Romina Schiaffino
  • Originador ●
  • Ponto De Contato
Centro de Investigaciones y transferencia del Noroeste de la Provincia de Buenos Aires
Junin
AR
Enrique Lara
  • Originador
University of Neuchâtel
Neuchâtel
CH
Leonardo Fernandez
  • Originador
University of Neuchâtel
Neuchâtel
CH
Vanessa Balagué
  • Originador
Institut de Ciències del Mar
Barcelona
ES
David Singer
  • Originador
University of Neuchâtel
Neuchâtel
CH
Christophe Seppey
  • Originador
University of Neuchâtel
Neuchâtel
CH
Ramon Massana
  • Originador
Institut de Ciències del Mar
Barcelona
ES
Irina Izaguirre
  • Originador
Universidad de Buenos Aires
Buenos Aires
AR
Maxime Sweetlove
  • Provedor Dos Metadados
  • Research assistent
Royal Belgian Institute of Natural Sciences
  • Rue Vautier 29
1000 Brussels
BE

Cobertura Geográfica

Latitudinal gradient from Patagonia and Terra del Fuego (Argentina) to the Antarctic Peninsula

Coordenadas delimitadoras Sul Oeste [-63,4, -71,51], Norte Leste [-45,55, -57]

Cobertura Taxonômica

microbial eukaryotes were profiled by targeting the 18S ssu rRNA gene (v9 region)

Domínio Eukaryota (Eukaryotes)

Cobertura Temporal

Data Inicial / Data final 2004-01-15 / 2008-10-20

Dados Sobre o Projeto

Nenhuma descrição disponível

Título microbial Eukaryotes in lakes along an Argentinian-Antarctic geographical gradient
Financiamento This work was supported by a grant from the Argentinean Funds for Technical and Scientific Investigation (FONCYT, PICT 32732 and PICT 2013‐0794), the Spanish Project MIXANTAR (REN 2002‐11396‐E/ANT), the Swiss NSF project 310003A_143960 and the Program ‘Luis Santaló’ of the National Research Council of Spain and the National Council of Scientific and Technical Research of Argentina (CSIC‐CONICET, PROBA 2007AR0018).

O pessoal envolvido no projeto:

Romina Schiaffino

Métodos de Amostragem

Integrated samples were collected within the euphotic zone from the surface down to 5 m in deep lakes and from about 30 cm below the surface in shallow lakes.

Área de Estudo Samples were collected once in 14 freshwater bodies (ranging from oligotrophic to eutrophic) from Chubut Province, Argentinean Patagonia, to Hope Bay, Antarctic Peninsula (45º22′ to 63º24′ S of latitude) (Fig. 1). In Antarctic lakes, samples were taken during the austral summer 2004, whereas in Patagonian lakes, samples were collected in late spring 2007 and 2008.

Descrição dos passos do método:

  1. Water samples were pre‐filtered in situ through a 50 µm net to remove zooplankton, then filtered with a vacuum pump first through a 20 µm pore‐size polycarbonate filter and then through a 3 µm and 0.2 µm pore‐size polycarbonate filters (diameter 47 mm; Millipore, Cork, Ireland). The filters were placed in cryovials with 1.8 ml of lysis buffer (40 mM EDTA, 50 mM Tris‐HCl, 0.75 M sucrose) and stored at −80°C until DNA extraction. The 0.2–3 µm size fraction was used for this study. The procedures followed for DNA extraction (phenol/chloroform extraction) and touchdown polymerase chain reaction (PCR) amplifications were previously described in detail (Unrein et al., 2005).
  2. We amplified extracted DNA using primers specific to the V9 variable region of the 18S rRNA gene using the protocol as in Amaral‐Zettler et al. (2009), and adapted after Lara et al. (2015). Sequencing was performed by the company Fasteris (Geneva, Switzerland) using Illumina HiSeq 2500 technology; paired end reads were around 200 bp in length.

Citações bibliográficas

  1. Schiaffino, M. R., Lara, E., Fernández, L. D., Balagué, V., Singer, D., Seppey, C. C., ... & Izaguirre, I. (2016). Microbial eukaryote communities exhibit robust biogeographical patterns along a gradient of Patagonian and Antarctic lakes. Environmental microbiology, 18(12), 5249-5264. https://doi.org/10.1111/1462-2920.13566