microbial Eukaryotes in lakes along an Argentinian-Antarctic geographical gradient

Metadata-only
Последняя версия опубликовано SCAR - Microbial Antarctic Resource System мар 20, 2019 SCAR - Microbial Antarctic Resource System
Дата публикации:
20 марта 2019 г.
Опубликовано:
SCAR - Microbial Antarctic Resource System
Лицензия:
CC-BY 4.0

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Описание

Amplicon sequencing dataset (Illumina HiSeq) of Eukaryotes (18S) in lakes along a latitudinal gradient from Southern Argentina to maritime Antarctica

Версии

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Как оформить ссылку

Исследователи должны дать ссылку на эту работу следующим образом:

Schiaffino R, Lara E, Fernandez L, Balagué V, Singer D, Seppey C, Massana R, Izaguirre I (2019): microbial Eukaryotes in lakes along an Argentinian-Antarctic geographical gradient. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbial_eukaryotes_in_antarctic_and_argentinian_lakes&v=1.0

Права

Исследователи должны соблюдать следующие права:

Публикующей организацией и владельцем прав на данную работу является SCAR - Microbial Antarctic Resource System. Эта работа находится под лицензией Creative Commons Attribution (CC-BY 4.0).

Регистрация в GBIF

Этот ресурс был зарегистрирован в GBIF, ему был присвоен следующий UUID: bc44c279-bfe6-4827-80b4-06dcb9aa7908.  SCAR - Microbial Antarctic Resource System отвечает за публикацию этого ресурса, и зарегистрирован в GBIF как издатель данных при оподдержке Scientific Committee on Antarctic Research.

Ключевые слова

Metadata

Контакты

Romina Schiaffino
  • Originator ●
  • Point Of Contact
Centro de Investigaciones y transferencia del Noroeste de la Provincia de Buenos Aires
Junin
AR
Enrique Lara
  • Originator
University of Neuchâtel
Neuchâtel
CH
Leonardo Fernandez
  • Originator
University of Neuchâtel
Neuchâtel
CH
Vanessa Balagué
  • Originator
Institut de Ciències del Mar
Barcelona
ES
David Singer
  • Originator
University of Neuchâtel
Neuchâtel
CH
Christophe Seppey
  • Originator
University of Neuchâtel
Neuchâtel
CH
Ramon Massana
  • Originator
Institut de Ciències del Mar
Barcelona
ES
Irina Izaguirre
  • Originator
Universidad de Buenos Aires
Buenos Aires
AR
Maxime Sweetlove
  • Metadata Provider
Research assistent
Royal Belgian Institute of Natural Sciences
Rue Vautier 29
1000 Brussels
BE

Географический охват

Latitudinal gradient from Patagonia and Terra del Fuego (Argentina) to the Antarctic Peninsula

Ограничивающие координаты Юг Запад [-63,4, -71,51], Север Восток [-45,55, -57]

Таксономический охват

microbial eukaryotes were profiled by targeting the 18S ssu rRNA gene (v9 region)

Domain Eukaryota (Eukaryotes)

Временной охват

Дата начала / Дата окончания 2004-01-15 / 2008-10-20

Данные проекта

Описание отсутсвует

Название microbial Eukaryotes in lakes along an Argentinian-Antarctic geographical gradient
Финансирование This work was supported by a grant from the Argentinean Funds for Technical and Scientific Investigation (FONCYT, PICT 32732 and PICT 2013‐0794), the Spanish Project MIXANTAR (REN 2002‐11396‐E/ANT), the Swiss NSF project 310003A_143960 and the Program ‘Luis Santaló’ of the National Research Council of Spain and the National Council of Scientific and Technical Research of Argentina (CSIC‐CONICET, PROBA 2007AR0018).

Исполнители проекта:

Romina Schiaffino

Методы сбора

Integrated samples were collected within the euphotic zone from the surface down to 5 m in deep lakes and from about 30 cm below the surface in shallow lakes.

Охват исследования Samples were collected once in 14 freshwater bodies (ranging from oligotrophic to eutrophic) from Chubut Province, Argentinean Patagonia, to Hope Bay, Antarctic Peninsula (45º22′ to 63º24′ S of latitude) (Fig. 1). In Antarctic lakes, samples were taken during the austral summer 2004, whereas in Patagonian lakes, samples were collected in late spring 2007 and 2008.

Описание этапа методики:

  1. Water samples were pre‐filtered in situ through a 50 µm net to remove zooplankton, then filtered with a vacuum pump first through a 20 µm pore‐size polycarbonate filter and then through a 3 µm and 0.2 µm pore‐size polycarbonate filters (diameter 47 mm; Millipore, Cork, Ireland). The filters were placed in cryovials with 1.8 ml of lysis buffer (40 mM EDTA, 50 mM Tris‐HCl, 0.75 M sucrose) and stored at −80°C until DNA extraction. The 0.2–3 µm size fraction was used for this study. The procedures followed for DNA extraction (phenol/chloroform extraction) and touchdown polymerase chain reaction (PCR) amplifications were previously described in detail (Unrein et al., 2005).
  2. We amplified extracted DNA using primers specific to the V9 variable region of the 18S rRNA gene using the protocol as in Amaral‐Zettler et al. (2009), and adapted after Lara et al. (2015). Sequencing was performed by the company Fasteris (Geneva, Switzerland) using Illumina HiSeq 2500 technology; paired end reads were around 200 bp in length.

Библиографические ссылки

  1. Schiaffino, M. R., Lara, E., Fernández, L. D., Balagué, V., Singer, D., Seppey, C. C., ... & Izaguirre, I. (2016). Microbial eukaryote communities exhibit robust biogeographical patterns along a gradient of Patagonian and Antarctic lakes. Environmental microbiology, 18(12), 5249-5264. https://doi.org/10.1111/1462-2920.13566

Дополнительные метаданные