Western Antarctic Peninsula Bacteria (Mesocosm exp.)
Latest version published by SCAR - Microbial Antarctic Resource System on 19 March 2019 SCAR - Microbial Antarctic Resource System

Amplicon sequencing data of the 16S SSU rRNA marker gene of bacteria in four Mesososm experiments that spanned the spring transitional period (August–December 2013) in surface waters of the Western Antarctic Peninsula (WAP). Each mesocosm consisted of nearshore surface seawater (50 L) incubated in the laboratory for 10 days. Further details in: Luria, C. M., Amaral-Zettler, L. A., Ducklow, H. W., Repeta, D. J., Rhyne, A. L., & Rich, J. J. (2017). Seasonal shifts in bacterial community responses to phytoplankton-derived dissolved organic matter in the Western Antarctic Peninsula. Frontiers in microbiology, 8, 2117.

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Luria C, Amaral-Zettler L, Ducklow H, Repeta D, Rhyne A, Rich J (2018): Western Antarctic Peninsula Bacteria (Mesocosm exp.). v1.4. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=western_peninsula_microbes&v=1.4

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Metadata

Contacts

Who created the resource:

Catherine Luria
Brown University
US
Linda Amaral-Zettler
Royal Netherlands Institute for Sea Research
NL
Hugh Ducklow
Lamont-Doherty Earth Observatory
US
Daniel Repeta
Woods Hole Oceanographic Institution
Woods Hole
US
Andrew Rhyne
Roger Williams University
Bristol
US
Jeremy Rich
University of Maine
Walpole
US

Who can answer questions about the resource:

Linda Amaral-Zettler
Royal Netherlands Institute for Sea Research
NL
Jeremy Rich
University of Maine
193 Clarks Cove Road
04573 Walpole
US

Who filled in the metadata:

Maxime Sweetlove
Assistent researcher
Royal Belgian Institude of Natural Sciences
Rue Vautier 29
1000 Brussels
BE
Geographic Coverage

Sea off the Western Antarctic Peninsula

Bounding Coordinates South West [-64.77, -64.18], North East [-64.77, -64.05]
Taxonomic Coverage

Bacteria (16S ssu rRNA gene, v6 region)

Domain  Bacteria (Bacteria)
Temporal Coverage
Formation Period 2009-2014
Project Data

No Description available

Title Western Antarctic Peninsula Bacteria (Mesocosm exp.)
Funding CL was partially funded by the Graduate School and the Department of Ecology and Evolutionary Biology at Brown University and the Brown University-Marine Biological Laboratory Joint Graduate Program. This material is based upon work supported by the National Science Foundation under Grant Nos. ANT-1142114 to LA-Z, OPP-0823101 and PLR-1440435 to HD, and ANT-1141993 to JR. The Gordon and Betty Moore Foundation grant 1711 supported work by DR.
Study Area Description Western Antarctic Peninsula
Design Description Mesocosm experiments were conducted in August, September, October, and December 2013 at Palmer Station by filling acid-washed 50 L polycarbonate carboys with seawater from the station’s intake. The filled carboys were divided into two treatments: (1) control (no additions, n = 3) and (2) +DOM (carboys that received diatom exudates; n = 3). An additional f/2 control treatment (uninoculated growth media, n = 3) was included in the October experiment only. The DOC concentration in the f/2 control was not any different than controls without any added DOC. As such, the f/2 control served as an additional no DOC addition control. The mesocosms were incubated for 10 days at 0°C, except in the August experiment when mesocosms were incubated at 3°C. All experiments were conducted under a low-level of continuous light, 1 × 1014 quanta cm-2 s-1, as measured inside the carboys with a hand-held radiometer (Biospherical Instruments, Inc.).

The personnel involved in the project:

Catherine Lucia
Linda Amaral-Zettler
Hugh Ducklow
Daniel Repeta
Andrew Rhyne
Jeremy Rich
Sampling Methods

Mesocosms were set up at Palmer Station, Antarctica, using seawater from the station’s intake, located at a depth of 6 m, 16 m from the shore of the station. This source water was monitored during the experimental period (August–December 2013) to determine any changes in environmental parameters that might influence bacterial community responses in mesocosm experiments. Samples for dissolved nutrients (phosphate, silicate, nitrite, and nitrate) and particulate organic carbon and nitrogen (POC and PN) were processed according to Palmer LTER standard protocols

Study Extent Western Antarctic Peninsula

Method step description:

  1. Mesocosms were set up at Palmer Station, Antarctica, using seawater from the station’s intake, located at a depth of 6 m, 16 m from the shore of the station. This source water was monitored during the experimental period (August–December 2013) to determine any changes in environmental parameters that might influence bacterial community responses in mesocosm experiments. Samples for dissolved nutrients (phosphate, silicate, nitrite, and nitrate) and particulate organic carbon and nitrogen (POC and PN) were processed according to Palmer LTER standard protocols
Bibliographic Citations
  1. Luria, C. M., Amaral-Zettler, L. A., Ducklow, H. W., Repeta, D. J., Rhyne, A. L., & Rich, J. J. (2017). Seasonal shifts in bacterial community responses to phytoplankton-derived dissolved organic matter in the Western Antarctic Peninsula. Frontiers in microbiology, 8, 2117.
Additional Metadata
Alternative Identifiers 7ebcc399-ad1f-4263-86a9-f612b1454a4a
https://ipt.biodiversity.aq/resource?r=western_peninsula_microbes