Description
Amplicon sequencing data of the 16S SSU rRNA marker gene of bacteria in four Mesososm experiments that spanned the spring transitional period (August–December 2013) in surface waters of the Western Antarctic Peninsula (WAP). Each mesocosm consisted of nearshore surface seawater (50 L) incubated in the laboratory for 10 days. Further details in: Luria, C. M., Amaral-Zettler, L. A., Ducklow, H. W., Repeta, D. J., Rhyne, A. L., & Rich, J. J. (2017). Seasonal shifts in bacterial community responses to phytoplankton-derived dissolved organic matter in the Western Antarctic Peninsula. Frontiers in microbiology, 8, 2117.
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How to cite
Researchers should cite this work as follows:
Luria C, Amaral-Zettler L, Ducklow H, Repeta D, Rhyne A, Rich J (2018): Western Antarctic Peninsula Bacteria (Mesocosm exp.). v1.4. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=western_peninsula_microbes&v=1.4
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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF Registration
This resource has been registered with GBIF, and assigned the following GBIF UUID: 7ebcc399-ad1f-4263-86a9-f612b1454a4a. SCAR - Microbial Antarctic Resource System publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Scientific Committee on Antarctic Research.
Keywords
Metadata
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Geographic Coverage
Sea off the Western Antarctic Peninsula
| Bounding Coordinates | South West [-64.77, -64.18], North East [-64.77, -64.05] |
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Taxonomic Coverage
Bacteria (16S ssu rRNA gene, v6 region)
| Domain | Bacteria (Bacteria) |
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Temporal Coverage
| Formation Period | 2009-2014 |
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Project Data
No Description available
| Title | Western Antarctic Peninsula Bacteria (Mesocosm exp.) |
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| Funding | CL was partially funded by the Graduate School and the Department of Ecology and Evolutionary Biology at Brown University and the Brown University-Marine Biological Laboratory Joint Graduate Program. This material is based upon work supported by the National Science Foundation under Grant Nos. ANT-1142114 to LA-Z, OPP-0823101 and PLR-1440435 to HD, and ANT-1141993 to JR. The Gordon and Betty Moore Foundation grant 1711 supported work by DR. |
| Study Area Description | Western Antarctic Peninsula |
| Design Description | Mesocosm experiments were conducted in August, September, October, and December 2013 at Palmer Station by filling acid-washed 50 L polycarbonate carboys with seawater from the station’s intake. The filled carboys were divided into two treatments: (1) control (no additions, n = 3) and (2) +DOM (carboys that received diatom exudates; n = 3). An additional f/2 control treatment (uninoculated growth media, n = 3) was included in the October experiment only. The DOC concentration in the f/2 control was not any different than controls without any added DOC. As such, the f/2 control served as an additional no DOC addition control. The mesocosms were incubated for 10 days at 0°C, except in the August experiment when mesocosms were incubated at 3°C. All experiments were conducted under a low-level of continuous light, 1 × 1014 quanta cm-2 s-1, as measured inside the carboys with a hand-held radiometer (Biospherical Instruments, Inc.). |
The personnel involved in the project:
Sampling Methods
Mesocosms were set up at Palmer Station, Antarctica, using seawater from the station’s intake, located at a depth of 6 m, 16 m from the shore of the station. This source water was monitored during the experimental period (August–December 2013) to determine any changes in environmental parameters that might influence bacterial community responses in mesocosm experiments. Samples for dissolved nutrients (phosphate, silicate, nitrite, and nitrate) and particulate organic carbon and nitrogen (POC and PN) were processed according to Palmer LTER standard protocols
| Study Extent | Western Antarctic Peninsula |
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Method step description:
- Mesocosms were set up at Palmer Station, Antarctica, using seawater from the station’s intake, located at a depth of 6 m, 16 m from the shore of the station. This source water was monitored during the experimental period (August–December 2013) to determine any changes in environmental parameters that might influence bacterial community responses in mesocosm experiments. Samples for dissolved nutrients (phosphate, silicate, nitrite, and nitrate) and particulate organic carbon and nitrogen (POC and PN) were processed according to Palmer LTER standard protocols
Bibliographic Citations
- Luria, C. M., Amaral-Zettler, L. A., Ducklow, H. W., Repeta, D. J., Rhyne, A. L., & Rich, J. J. (2017). Seasonal shifts in bacterial community responses to phytoplankton-derived dissolved organic matter in the Western Antarctic Peninsula. Frontiers in microbiology, 8, 2117.
Additional Metadata
| Alternative Identifiers | 7ebcc399-ad1f-4263-86a9-f612b1454a4a |
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| https://ipt.biodiversity.aq/resource?r=western_peninsula_microbes |