Geothermal Fumarole Subsurface Mt. Erebus, Antarctica

最新バージョン SCAR - Microbial Antarctic Resource System によって公開 Oct 19, 2015 SCAR - Microbial Antarctic Resource System

The Tramway Ridge geothermal site on Mt. Erebus in Antarctica, is the most geographically isolated geothermal site on earth. This makes it an excellent system for studies of microbial speciation, biogeography, and evolution.

データ レコード

この オカレンス(観察データと標本) リソース内のデータは、1 つまたは複数のデータ テーブルとして生物多様性データを共有するための標準化された形式であるダーウィン コア アーカイブ (DwC-A) として公開されています。 コア データ テーブルには、40 レコードが含まれています。

この IPT はデータをアーカイブし、データ リポジトリとして機能します。データとリソースのメタデータは、 ダウンロード セクションからダウンロードできます。 バージョン テーブルから公開可能な他のバージョンを閲覧でき、リソースに加えられた変更を知ることができます。

ダウンロード

DwC-A形式のリソース データまたは EML / RTF 形式のリソース メタデータの最新バージョンをダウンロード:

DwC ファイルとしてのデータ ダウンロード 40 レコード English で (5 KB) - 更新頻度: unknown
EML ファイルとしてのメタデータ ダウンロード English で (10 KB)
RTF ファイルとしてのメタデータ ダウンロード English で (11 KB)

バージョン

次の表は、公にアクセス可能な公開バージョンのリソースのみ表示しています。

引用方法

研究者はこの研究内容を以下のように引用する必要があります。:

Herbold, CW; Lee, CK; McDonald, IR; Cary, SC; Evidence of global-scale aeolian dispersal and endemism in isolated geothermal microbial communities of Antarctica; Nature Comms (2014); 5

GBIF登録

このリソースをはGBIF と登録されており GBIF UUID: d2744050-427f-4b3f-824d-e96b7bd26368が割り当てられています。   Scientific Committee on Antarctic Research によって承認されたデータ パブリッシャーとして GBIF に登録されているSCAR - Microbial Antarctic Resource System が、このリソースをパブリッシュしました。

キーワード

Occurrence; Observation

連絡先

リソースを作成した人:

Craig Herbold
Group Leader
Division of Microbial Ecology (DoME), University of Vienna Althanstrasse 14 1090 Vienna AT
http://www.microbial-ecology.net/

リソースに関する質問に答えることができる人:

S. Craig Cary
Professor
University of Waikato Private Bag 3105 3105 Hamilton NZ
http://www.ictar.aq/ictar_leadership.cfm?staff_id=1&page_obj_id=246&obj_list=1

メタデータを記載した人:

Craig Herbold
Group Leader
Division of Microbial Ecology (DoME), University of Vienna Althanstrasse 14 1090 Vienna AT
http://www.microbial-ecology.net/

地理的範囲

ASPA 130 - Tramway Ridge Specially Protected Area on the northwest summit of Mt. Erebus, Antarctica

座標(緯度経度) 南 西 [-77.519, 167.106], 北 東 [-77.517, 167.114]

時間的範囲

開始日 2009-02-09

プロジェクトデータ

説明がありません

タイトル Life at the extreme: resolving the genetic basis of microbial endemism in the super-heated soils of Mt Erebus, Antarctica
ファンデイング Financial support was provided by grant UOW0802 from the New Zealand Marsden Fund to SCC and IRM and a CRE award from the National Geographic Society to SCC. Antarctic logistic support for Event K-023 was provided by Antarctica New Zealand.
Study Area Description The summit of Mt. Erebus features several high-elevation geothermal features that are separated from similar features at Mts. Melbourne and Rittman by 350-400 km. Warm fumarolic ground and ice towers on the flanks of Mt. Erebus passively emit steam and CO2 that are believed to have magmatic origins(Wardell et al., 2003). The lower end of Tramway Ridge, located approximately 1.5 km NW of the main crater of Erebus, at an elevation between 3350 and 3400 m, is an extensive warm fumarolic area protected by international treaty as a site of particular biological interest (ASPA 130 Management Plan). At Tramway Ridge, unique communities of photoautotrophic organisms (mosses and cyanobacterial mats) surround fumaroles that reach and maintain year round surface temperatures of 60-65°C, have a neutral to mildly alkaline pH, and are characterized by steep lateral pH and temperature gradients (Broady, 1984; Hudson et al., 1989; Soo et al., 2009).
研究の意図、目的、背景など(デザイン) The current study aimed to fully characterize the distribution of microbial communities inhabiting the 60-65°C sites of the CO2-emitting fumaroles located at Tramway Ridge, and to identify the set of organisms specifically associated with the subsurface. To meet these objectives, a mixed amplicon /metagenomic pyrosequencing-based approach was employed. Correlated relative abundance, as calculated from 16S rRNA gene amplicon libraries was used to identify sets of organisms that occupy shared niches. A pooled shotgun-metagenomic dataset was then used to both verify the abundance of dominant organisms and reconstruct whole 16S rRNA genes, allowing more comprehensive phylogenetic analyses.

プロジェクトに携わる要員:

研究代表者
S. Craig Cary

収集方法

Sediment samples were collected within the Tramway Ridge Antarctic Specially Protected Area (ASPA 130) in February 2009 from two sites (site A: 77° 31.103' S, 167° 6.682' S and site B: 77° 31.106' S, 167° 6.668' E). All suggested sterilization protocols for entering into this protected site were adhered to, following the ASPA 130 Management Plan (http://www.scar.org/publications/bulletins/151/aspa130.html). Sites were chosen based on measuring a surface temperature of 65°C with a stainless steel Checktemp1 temperature probe (Hanna Instruments, Rhode Island, USA), sterilized with 70% ethanol immediately prior to each use. Surface "crust" was set aside prior to collecting samples. Samples were collected by aseptically removing the top 2 cm of sediment in an approximately 25cm2 area. Sediment was placed into a fresh 50 mL Falcon tube. Sampling continued with the collection of a second (2-4 cm depth) and third (4-8 cm depth) layer following the same procedures. Temperature measurements were repeated for each layer sampled. All samples were immediately frozen and maintained at -80°C in the laboratory until analysed.

Study Extent The study encompassed two locations in the permitted area of the Tramway Ridge ASPA 130. http://www.nature.com/ncomms/2014/140520/ncomms4875/fig_tab/ncomms4875_F1.html
Quality Control Sampling conditions resulted in a high probability of cross-contamination between sampling depths. We used standard quality filtering for our 454 datasets, including denoising and chimera removal with AmpliconNoise. Sequence abundances, as inferred from library abundance, were used to cluster individual OTUs into correlated clusters which defined whether a given sequence was likely of surface or subsurface origin. Shotgun metagenomics was utilized to extract full-length 16S rRNA sequences for high-resolution phylogenetic analysis.

Method step description:

  1. /

コレクションデータ

コレクション名 Geothermal fumarole subsurface microbial communities from Mt. Erebus, Antarctica
コレクション識別子 http://www.ncbi.nlm.nih.gov/nuccore/KF923316,KF923317,KF923318,KF923319,KF923320,KF923321,KF923322,KF923323,KF923324,KF923325,KF923326,KF923327
Parent Collection Identifier Not Applicable
標本保存方法 Deep frozen

追加のメタデータ

代替識別子 d2744050-427f-4b3f-824d-e96b7bd26368
http://ipt.biodiversity.aq/resource.do?r=geothermal_fumarole_subsurface_mt_erebus_antarctica