Antarctic polar desert surface soil microbiome

Somente metadatos
Versão mais recente published by SCAR - Microbial Antarctic Resource System on mar 28, 2019 SCAR - Microbial Antarctic Resource System
Publication date:
28 de Março de 2019
Licença:
CC-BY 4.0

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Descrição

Whole-genome shotgun sequencing dataset targeting microbes in three soil samples from pristine polar desert at Robinson Ridge (eastern Antarctica)

Versões

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Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Ji M, Greening C, Vanwonterghem I, Carere C, Bay S, Steen J, Montgomery K, Lines T, van Dorst J, Snape I, Stott M, Hugenholtz P, Ferrari B (2019): Antarctic polar desert surface soil microbiome. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=antarctic_desert_surface_soil_microbiome&v=1.0

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: ef36ddd4-b37c-4467-9ad4-eb77d679959b.  SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.

Palavras-chave

Metadata

Contatos

Mukan Ji
  • Originador ●
  • Ponto De Contato
Australian Centre for Astrobiology
Randwick
AU
Chris Greening
  • Originador ●
  • Ponto De Contato
Monash University
Clayton
AU
Inka Vanwonterghem
  • Originador
University of Queensland
St Lucia
AU
Carlo Carere
  • Originador
Wairakei Research Centre
Wairakei
NZ
Sean Bay
  • Originador
Monash University
Clayton
AU
Jason Steen
  • Originador
University of Queensland
St. Lucia
AU
Kate Montgomery
  • Originador
Australian Centre for Astrobiology
Randwick
AU
Thomas Lines
  • Originador
Monash University
Clayton
AU
Josie van Dorst
  • Originador
Australian Centre for Astrobiology
Randwick
AU
Ian Snape
  • Originador
Australian Antarctic Division
Kingston
AU
Matthew Stott
  • Originador
Wairakei Research Centre
Wairakei
NZ
Philip Hugenholtz
  • Originador
University of Queensland
St. Lucia
AU
Belinda Ferrari
  • Originador ●
  • Ponto De Contato
Australian Centre for Astrobiology
Randwick
AU
Maxime Sweetlove
  • Provedor Dos Metadados
Research assistent
Royal Belgian Institute of Natural Sciences
Rue Vautier 29
1000 Brussels
BE

Cobertura Geográfica

Robinson Ridge, east Antarctica

Coordenadas delimitadoras Sul Oeste [-66,37, 110,586], Norte Leste [-66,37, 110,586]

Cobertura Taxonômica

whole genome shotgun sequencing

Domínio Bacteria (Bacteria), Archaea (Archaea), Eukaryota (Eukaryotes), Viri (viruses)

Cobertura Temporal

Data Inicial 2015-12-13

Dados Sobre o Projeto

Nenhuma descrição disponível

Título Bioplatforms Australia
Financiamento This work was supported by Bioplatforms Australia, an Australian Antarctic Science project grant (4406), an ARC Future Fellowship (FT170100341), an ARC DECRA Fellowship (DE170100310), an ARC DORA and Laureate Fellowship (DP120103498 and FL150100038), and a Marsden Grant (16-GNS-035). UNSW Sydney, the Centre for Geometric Biology (Monash University), and the Geothermal Resources of New Zealand research program (GNS Science) also provided funding to support this research.

O pessoal envolvido no projeto:

Mukan Ji

Métodos de Amostragem

Samples were collected along a spatially explicit sampling design comprised of three 300-m-long transects, separated by 2-m distances from each other. Samples were sieved down to 63 μm, aliquoted into 5–25-g subsamples, and stored at −80 °C until analysis.

Área de Estudo samples were taken at the ice free Robinson Ridge (−66.367739, 110.585262), located 10 km south of Casey station in the Windmill Islands coast of Wilkes Land, is part of a pristine polar desert. Three surface soil samples (100 g) from the top 10 cm of the soil profile were collected in December 2005.

Descrição dos passos do método:

  1. Total community DNA was extracted from 0.25–0.3 g of each sample in technical triplicate using the FastDNA SPIN Kit for Soil (MP Biomedicals). All DNA extracts were quantified and DNA lysate quality was evaluated using automated ribosomal intergenic spacer analysis (ARISA).
  2. Metagenome libraries were prepared using the Nextera DNA Library Preparation Kit (Illumina) and sequenced using three-fifths of an Illumina HiSeq2000 flowcell lane at the Institute for Molecular Biosciences (University of Queensland).

Citações bibliográficas

  1. Ji, M., Greening, C., Vanwonterghem, I., Carere, C. R., Bay, S. K., Steen, J. A., ... & Snape, I. (2017). Atmospheric trace gases support primary production in Antarctic desert surface soil. Nature, 552(7685), 400. https://doi.org/10.1038/nature25014