Antarctic polar desert surface soil microbiome

最新版本 published by SCAR - Microbial Antarctic Resource System on 三月 28, 2019 SCAR - Microbial Antarctic Resource System
發布日期:
2019年3月28日
授權條款:
CC-BY 4.0

下載最新版本資源元數據的EML或RTF文字檔。

元數據EML檔 下載 在 English 中 (12 KB)
元數據RTF文字檔 下載 在 English 中 (12 KB)

說明

Whole-genome shotgun sequencing dataset targeting microbes in three soil samples from pristine polar desert at Robinson Ridge (eastern Antarctica)

版本

以下的表格只顯示可公開存取資源的已發布版本。

如何引用

研究者應依照以下指示引用此資源。:

Ji M, Greening C, Vanwonterghem I, Carere C, Bay S, Steen J, Montgomery K, Lines T, van Dorst J, Snape I, Stott M, Hugenholtz P, Ferrari B (2019): Antarctic polar desert surface soil microbiome. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=antarctic_desert_surface_soil_microbiome&v=1.0

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF 註冊

此資源已向GBIF註冊,並指定以下之GBIF UUID: ef36ddd4-b37c-4467-9ad4-eb77d679959b。  SCAR - Microbial Antarctic Resource System 發佈此資源,並經由Scientific Committee on Antarctic Research同意向GBIF註冊成為資料發佈者。

關鍵字

Metadata

聯絡資訊

Mukan Ji
  • 出處
  • 連絡人
Australian Centre for Astrobiology
Randwick
AU
Chris Greening
  • 出處
  • 連絡人
Monash University
Clayton
AU
Inka Vanwonterghem
  • 出處
University of Queensland
St Lucia
AU
Carlo Carere
  • 出處
Wairakei Research Centre
Wairakei
NZ
Sean Bay
  • 出處
Monash University
Clayton
AU
Jason Steen
  • 出處
University of Queensland
St. Lucia
AU
Kate Montgomery
  • 出處
Australian Centre for Astrobiology
Randwick
AU
Thomas Lines
  • 出處
Monash University
Clayton
AU
Josie van Dorst
  • 出處
Australian Centre for Astrobiology
Randwick
AU
Ian Snape
  • 出處
Australian Antarctic Division
Kingston
AU
Matthew Stott
  • 出處
Wairakei Research Centre
Wairakei
NZ
Philip Hugenholtz
  • 出處
University of Queensland
St. Lucia
AU
Belinda Ferrari
  • 出處
  • 連絡人
Australian Centre for Astrobiology
Randwick
AU
Maxime Sweetlove
  • 元數據提供者
Research assistent
Royal Belgian Institute of Natural Sciences
Rue Vautier 29
1000 Brussels
BE

地理涵蓋範圍

Robinson Ridge, east Antarctica

界定座標範圍 緯度南界 經度西界 [-66.37, 110.586], 緯度北界 經度東界 [-66.37, 110.586]

分類群涵蓋範圍

whole genome shotgun sequencing

Domain Bacteria (Bacteria), Archaea (Archaea), Eukaryota (Eukaryotes), Viri (viruses)

時間涵蓋範圍

起始日期 2015-12-13

計畫資料

無相關描述

計畫名稱 Bioplatforms Australia
經費來源 This work was supported by Bioplatforms Australia, an Australian Antarctic Science project grant (4406), an ARC Future Fellowship (FT170100341), an ARC DECRA Fellowship (DE170100310), an ARC DORA and Laureate Fellowship (DP120103498 and FL150100038), and a Marsden Grant (16-GNS-035). UNSW Sydney, the Centre for Geometric Biology (Monash University), and the Geothermal Resources of New Zealand research program (GNS Science) also provided funding to support this research.

參與計畫的人員:

Mukan Ji

取樣方法

Samples were collected along a spatially explicit sampling design comprised of three 300-m-long transects, separated by 2-m distances from each other. Samples were sieved down to 63 μm, aliquoted into 5–25-g subsamples, and stored at −80 °C until analysis.

研究範圍 samples were taken at the ice free Robinson Ridge (−66.367739, 110.585262), located 10 km south of Casey station in the Windmill Islands coast of Wilkes Land, is part of a pristine polar desert. Three surface soil samples (100 g) from the top 10 cm of the soil profile were collected in December 2005.

方法步驟描述:

  1. Total community DNA was extracted from 0.25–0.3 g of each sample in technical triplicate using the FastDNA SPIN Kit for Soil (MP Biomedicals). All DNA extracts were quantified and DNA lysate quality was evaluated using automated ribosomal intergenic spacer analysis (ARISA).
  2. Metagenome libraries were prepared using the Nextera DNA Library Preparation Kit (Illumina) and sequenced using three-fifths of an Illumina HiSeq2000 flowcell lane at the Institute for Molecular Biosciences (University of Queensland).

引用文獻

  1. Ji, M., Greening, C., Vanwonterghem, I., Carere, C. R., Bay, S. K., Steen, J. A., ... & Snape, I. (2017). Atmospheric trace gases support primary production in Antarctic desert surface soil. Nature, 552(7685), 400. https://doi.org/10.1038/nature25014