Stable isotope ratios of C, N and S in Southern Ocean sea stars (1985-2017)
Latest version published by SCAR - AntOBIS on 02 September 2021 SCAR - AntOBIS

This dataset is a compilation of stable isotope ratios of C, N and S in tissues of 2456 sea stars sampled from 1985 to 2017 in the Southern Ocean (Antarctica and Subantarctic Islands). Stable isotope values were measured in the framework of Baptiste Le Bourg's PhD thesis at University of Liège, entitled “Trophic ecology of Southern Ocean sea stars: Influence of environmental drivers on trophic diversity”. Samples were provided by the University of Liège (Belgium), the Université Libre de Bruxelles (Belgium), the National Museum of Natural History (Paris, France) and the Institute of Oceanology of the Polish Academy of Sciences (Sopot, Poland). There could be duplicate records from collections of these institutes published on GBIF and OBIS. This work was supported by BELSPO through the vERSO and RECTO projects (contracts no. BR/132/A1/vERSO and BR/154/A1/RECTO). This dataset is published by SCAR-AntOBIS under the license CC-BY 4.0. Please follow the guidelines from the SCAR and IPY Data Policies (https://www.scar.org/excom-meetings/xxxi-scar-delegates-2010-buenos-aires-argentina/4563-scar-xxxi-ip04b-scar-data-policy/file/) when using the data. If you have any questions regarding this dataset, don't hesitate to contact us via the contact information provided in the metadata or via data-biodiversity-aq@naturalsciences.be. Issues with dataset can be reported at https://github.com/biodiversity-aq/data-publication/

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Data Records

The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 2,456 records.

1 extension data tables also exist. An extension record supplies extra information about a core record. The number of records in each extension data table is illustrated below.

  • Occurrence (core)
    2456
  • MeasurementOrFact 
    24335

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

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Data as a DwC-A file download 2,456 records in English (213 kB) - Update frequency: unknown
Metadata as an EML file download in English (24 kB)
Metadata as an RTF file download in English (22 kB)
Versions

The table below shows only published versions of the resource that are publicly accessible.

How to cite

Researchers should cite this work as follows:

Moreau C, Le Bourg B, Balazy P, Danis B, Eléaume M, Jossart Q, Kuklinski P, Lepoint G, Saucède T, Van de Putte A, Gan Y, Michel L (2021): Stable isotope ratios of C, N and S in Southern Ocean sea stars (1985-2017). v1.5. SCAR - AntOBIS. Dataset/Occurrence. https://ipt.biodiversity.aq/resource?r=antarctic_subantarctic_asteroidea_isotopes&v=1.5

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is SCAR - AntOBIS. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: ff3984d7-84bd-4f3a-b843-666faa4c1696.  SCAR - AntOBIS publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Ocean Biodiversity Information System.

Keywords

SEA STARS; ECHINODERMS; ISOTOPES; Specimen; Occurrence

Contacts

Who created the resource:

Camille Moreau
PostDoc
Université Libre de Bruxelles
BE
Baptiste Le Bourg
University of Liège
BE
Piotr Balazy
Polish Academy of Sciences
PL
Bruno Danis
Université libre de Bruxelles
BE
Marc Eléaume
Muséum National d'Histoire Naturelle
FR
Quentin Jossart
Université Libre de Bruxelles
BE
Piotr Kuklinski
Polish Academy of Sciences
PL
Giles Lepoint
University of Liège
BE
Thomas Saucède
Université Bourgogne Franche-Comté
FR
Anton Van de Putte
Royal Belgian Institute of Natural Sciences
BE
Yi-Ming Gan
Royal Belgian Institute of Natural Sciences
BE
Loïc Michel

Who can answer questions about the resource:

Baptiste Le Bourg
University of Liège
BE
Camille Moreau
Yi-Ming Gan

Who filled in the metadata:

Camille Moreau
Baptiste Le Bourg
University of Liège
BE
Geographic Coverage

Antarctic and Sub-Antarctic regions of the Southern Ocean

Bounding Coordinates South West [-76.715, -127.267], North East [-47.717, 162.201]
Taxonomic Coverage

Echinodermata, Asteroidea

Kingdom  Animalia
Phylum  Echinodermata
Class  Asteroidea (Sea star)
Order  Brisingida,  Forcipulatida,  Notomyotida,  Paxillosida,  Spinulosida,  Valvatida,  Velatida
Temporal Coverage
Start Date / End Date 1985-01-11 / 2017-10-08
Project Data

The data presented here were obtained in the framework of a PhD project. The goal of this PhD project was to study the trophic ecology of sea stars in the Southern Ocean using stable isotopes of carbon, nitrogen and sulfur. In particular, the relationship between trophic ecology and environmental conditions was assessed. The functioning of Southern Ocean ecosystems and the impact of climate change on them was thus a central theme of the PhD thesis. Indeed, changes in the availability of trophic resources induced by environmental changes can lead to changes of trophic ecology of species and of trophic interactions between them.

Title Trophic ecology of Southern Ocean sea stars: Influence of environmental drivers on trophic diversity
Identifier BR/154/A1/RECTO
Funding This project was funded thanks to a FRIA doctoral grant (Fund for research training in industry and agriculture) provided by the FRS-FNRS. This PhD thesis was carried out as part of the vERSO (Ecosystem Responses to global change: a multiscale approach in the Southern Ocean; BR/132/A1/vERSO) and RECTO (Refugia and Ecosystem Tolerance in the Southern Ocean; BR/154/A1/RECTO) projects, funded by the Belgian Science Policy Office (BELSPO). Other funders include: National Science Center Poland OPUS grant number 2020/37/B/ST10/02905: Influence of rapidly progressing climate change on polar marine organisms - investigations along naturally occurring environmental analogues of future climate changes French Polar Institute (IPEV) IPEV research program 1124 REVOLTA - Ressources Ecologiques et Valorisation par un Observatoire à Long terme en Terre Adélie Australian Antarctic Division (AAD) IPY project 53 CEAMARC - Collaborative East Antarctic MARine Census

The personnel involved in the project:

Author
Baptiste Le Bourg
Sampling Methods

A double sampling strategy was set up to maximise the scope of this PhD thesis. First, sea stars were collected in the Southern Ocean during campaigns taking place in the framework of the vERSO and RECTO projects from December 2015 to March 2017. Second, suitable samples originating from multiple oceanographic campaigns and surveys from January 1985 to January 2015 were retrieved from archived collections stored in museums or institutions. Institutions that provided samples included the Université Libre de Bruxelles (Belgium), the National Museum of Natural History (Paris, France) and the Institute of Oceanology of the Polish Academy of Sciences (Sopot, Poland). Storage: Depending of the sampling campaign, sea stars were frozen, dried, stored in ethanol or fixed with formaldehyde and then stored in ethanol.

Study Extent Sea stars were sampled in the Southern Ocean from 1985 to 2017 throughout multiple oceanographic campaigns.
Quality Control - Isotopic ratios were expressed in ‰ using the widespread δ notation (Coplen 2011) and relative to the international references Vienna Pee Dee Belemnite (for carbon), Atmospheric Air (for nitrogen) and Vienna Canyon Diablo Troilite (for sulfur). Sucrose (IAEA-C-6; δ13C=−10.8 ± 0.5‰; mean ± standard deviation), ammonium sulfate (IAEA-N-1; δ15N= 0.4 ± 0.2‰; mean ± SD) and silver sulfide (IAEA-S-1; δ34S = −0.3‰) were used as primary analytical standards for stable isotope ratios. Sulfanilic acid (Sigma-Aldrich; δ13C=−25.6 ± 0.4‰; δ15N=−0.13 ± 0.4‰; δ34S = 5.9 ± 0.5‰; means ± SD) was used as a secondary analytical standard for stable isotope ratios and as elemental standard. Standard deviations on multi-batch replicate measurements of secondary and internal laboratory standards (sea star tegument), analyzed interspersed with samples (one replicate of each standard every 15 analyses), were 0.3‰ for δ13C and δ15N and 0.5‰ for δ34S. - All records were validated. - Coordinates were plotted on map to verify the geographical location and locality. - All scientific names were checked for typo and matched to the species information backbone of Worlds Register of Marine Species (http://marinespecies.org/) and LSID were assigned to each taxa as scientificNameID. - Event date and time were converted into ISO 8601

Method step description:

  1. Sample preparation: For each sea star, one or several arms were separated from the central disc. Internal organs and podia were removed in each arm. With the exception of the already dried samples, the tegument and the podia of each arm were washed with demineralised water and oven-dried at 50°C during 48 hours. All samples were then homogenised into powder. Carbonates were removed from subsamples by exposing subsamples to 37 % hydrochloric acid vapour during 48 hours. Acidified subsamples were then kept at 60°C until further sample preparation.
  2. Stable isotope analysis: The subsamples were then precisely weighed (ca 2.5-3 mg) in 5×8 tin cups with ca 3 mg of tungsten trioxide. Their elemental composition and their stable isotope values were analysed with an elemental analyser coupled to a continuous-flow isotope-ratio mass spectrometer.
  3. Impact of preservation on stable isotope values: Correction factors were added to the δ13C and δ34S values of sea stars fixed with formaldehyde and/or stored in ethanol as fixation in formaldehyde and preservation in ethanol impact stable isotope values in sea stars (Le Bourg et al., 2020). For samples stored in ethanol, a correction factor of –0.6 ‰ was subtracted to δ13C values. For samples fixed with formaldehyde and then stored in ethanol, a correction factor of 0.2 ‰ was added to δ13C values to take into account the effects of both ethanol (–0.6 ‰) and formaldehyde (+0.8 ‰) on δ13C values. A correction factor of 1.5 ‰ was also added to δ34S values for samples fixed with formaldehyde.
  4. Instrumentation: Stable isotope ratio measurements were performed by continuous flow–elemental analysis–isotope ratio mass spectrometry (CF-EA-IRMS) at University of Liège (Belgium), using a vario MICRO cube C-N-S elemental analyzer (Elementar Analysensysteme GmbH, Hanau, Germany) coupled to an IsoPrime100 isotope ratio mass spectrometer (Isoprime, Cheadle, United Kingdom).
  5. Taxonomy and systematics: In the laboratory, sea stars were identified to the lowest taxonomic level possible either using morphological or molecular characters. Moreover, in several genera where clades showed a clear pattern of geographic or bathymetric distribution, results of genetic analyses were used as proxies to assign specimens to a probable species (Moreau 2019, Moreau et al. 2019). Bathybiaster sp. individuals sampled between 0 and 1000 m on the Antarctic continental shelf were considered as Bathybiaster loripes, those sampled deeper than 2000 m, as well as on the Kerguelen Plateau were considered as Bathybiaster vexillifer (Moreau 2019) while no species was assigned to one individual sampled in the South Sandwich Islands between 1000 and 1500 m. Chitonaster sp. individuals sampled in Western Antarctic Peninsula and South Orkney Island were considered as Chitonaster sp. 2. Diplasterias sp. individuals sampled in Western Antarctic Peninsula and South Orkney Island were considered as Diplasterias sp. 1, those sampled in South Sandwich Islands and Adélie Land as Diplasterias sp. 2 while no species was assigned to individuals sampled in the Weddell Shelf. Lysasterias sp. individuals sampled in Adélie Land were considered as Lysasterias sp. 1. Notasterias sp. individuals sampled in South Orkney Islands were considered as Notasterias sp. 1. Odontaster sp. individuals sampled on the Kerguelen Plateau were considered as Odontaster penicillatus. Psilaster charcoti individuals sampled near Bouvet Island, in the South Atlantic ecoregion, were considered as Psilaster charcoti – clade 2. By contrast, this method could not be used for genera for which no clear geographic or bathymetric patterns of distribution were recorded (e.g. Acodontaster). Consequently, these individuals remained identified down to the genus. Similarly, individuals that could not be identified further than the family were referred to by their family name (Echinasteridae and Pterasteridae). Nomenclature was thoroughly checked using the Taxon Match Tool implemented in the World Register of Marine Species, WoRMS (WoRMS Editorial Board 2021).
Bibliographic Citations
  1. Le Bourg, Baptiste, Gilles Lepoint, and Loïc N. Michel. 2020. “Effects of Preservation Methodology on Stable Isotope Compositions of Sea Stars.” Rapid Communications in Mass Spectrometry 34 (2). ‌ https://doi.org/10.1002/rcm.8589.
  2. Camille Moreau. Diversity and phylogeography of Southern Ocean sea stars (Asteroidea). Biodiversity and Ecology. Université Bourgogne Franche-Comté; Université libre de Bruxelles (1970-..), 2019. English. ⟨NNT : 2019UBFCK061⟩. ⟨tel-02489002⟩
  3. Moreau, C, Danis, B, Jossart, Q, et al. Is reproductive strategy a key factor in understanding the evolutionary history of Southern Ocean Asteroidea (Echinodermata)? Ecol Evol. 2019; 9: 8465– 8478. https://doi.org/10.1002/ece3.5280
  4. Coplen, T.B. (2011), Guidelines and recommended terms for expression of stable-isotope-ratio and gas-ratio measurement results. Rapid Commun. Mass Spectrom., 25: 2538-2560. https://doi.org/10.1002/rcm.5129
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