Bacterioplankon from lakes on Byers Peninsula (2008)

Somente metadatos
Versão mais recente published by SCAR - Microbial Antarctic Resource System on jun 16, 2021 SCAR - Microbial Antarctic Resource System
Publication date:
16 de junho de 2021
Licença:
CC-BY 4.0

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Descrição

Amplicon sequencing dataset (Illumina MiSeq) targeting Bacteria (16S ssu rRNA) in samples (n=9) taken in 2008 from Byers Peninsula (Antarctica).

Versões

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Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Picazo A, Rochera C, Villaescusa J A, Javier Miralles-Lorenzo J, Velazquez D, Quesada A, Camacho A (2021): Bacterioplankon from lakes on Byers Peninsula (2008). v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=bacterioplankon_byers_peninsula_lakes_2008&v=1.0

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 625b1148-c88d-459b-b3e7-d7504cc6d97d.  SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.

Palavras-chave

Metadata

Contatos

Antonio Picazo
  • Originador
University of Valencia
Valencia
ES
Carlos Rochera
  • Originador
University of Valencia
Valencia
ES
Juan Antonio Villaescusa
  • Originador
University of Valencia
Valencia
ES
Javier Javier Miralles-Lorenzo
  • Originador
University of Valencia
Valencia
ES
David Velazquez
  • Originador
Universidad Autónoma de Madrid
Madrid
ES
Antonio Quesada
  • Originador
Universidad Autónoma de Madrid
Madrid
ES
Antonio Camacho
  • Originador ●
  • Usuário ●
  • Ponto De Contato
University of Valencia
Valencia
ES
Maxime Sweetlove
  • Provedor Dos Metadados
Royal Belgian Institute of Natural Sciences
Brussels
BE

Cobertura Geográfica

Lakes from Beyers Peninsula (Antarctica)

Coordenadas delimitadoras Sul Oeste [-62,661, -61,112], Norte Leste [-62,631, -61,006]

Cobertura Temporal

Data Inicial / Data final 2008-01-28 / 2008-02-08

Dados Sobre o Projeto

Nenhuma descrição disponível

Título Bacterioplankon from lakes on Byers Peninsula (2008)
Financiamento This study was supported by grants CGL2005-06549-C02-02/ANT to AC and CTM2016-79741-R to AQ, both funded by the Spanish Ministry of Education and Science, by European FEDER funds, a way to make Europe.

O pessoal envolvido no projeto:

Antonio Camacho

Métodos de Amostragem

Samples in all lakes were obtained from surface depths (0.5–1 m), and approximately in the center of the lake. Samples were then filtered with a vacuum system onto 0.2 μm polycarbonate filters (Nucleopore, Whatman) and stored frozen (−20°C) in Allprotect Tissue Reagent (QIAGEN).

Área de Estudo Water samples (n=9) were collected during January and February 2008, in lakes Chester Cone, Midge Lake, Escondido, Limnopolar, Turbio, Somero, and Refugio (Beyers Peninsula, Antarctica)
Controle de Qualidade The extracted and amplified DNA pool was subjected to an analysis with a Qubit dsDNA HS, Agilent 4200 TapeStation High Sensitivity DNA and Kapa Illumina Library Quantification qPCR assays.

Descrição dos passos do método:

  1. DNA extraction from each filter was performed with the EZNA Soil DNA isolation kit (Omega Bio-Tek, Inc., Norcross, GA, United States) following the instructions given by the supplier. Sequencing of the region V4 of the 16S rRNA gene was done using the Illumina MiSeq system (2×250 bp) at the genomics facilities of the Research Technology Support Facility of the Michigan State University, United States. For each sample, Illumina compatible, dual indexed amplicon libraries of the 16S-V4 rRNA hypervariable region were created with primers 515f/806r. PCR reactions are composed of 5 μL of 4 μM equimolar primer set, 0.15 μL of AccuPrime Taq DNA High Fidelity Polymerase, 2 μL of 10× AccuPrime PCR Buffer II (Thermo Fisher Scientific, catalog no. 12346094), 11.85 μL of PCR-grade water, and 1 μL of DNA template. The PCR conditions used consisted of 2 min at 95°C, followed by 30 cycles of 95°C for 20 s, 55°C for 15 s, and 72°C for 5 min, followed by 72°C for 10 min.
  2. Completed libraries were batch normalized using Invitrogen SequalPrep DNA Normalization Plates. The DNA was sequenced on an 2x250bp Illumina MiSeq v2 flow cell using a MiSeq v2 500 cycle reagent cartridge.

Citações bibliográficas

  1. Picazo, A., Rochera, C., Villaescusa, J. A., Miralles-Lorenzo, J., Velázquez, D., Quesada, A., & Camacho, A. (2019). Bacterioplankton community composition along environmental gradients in lakes from Byers Peninsula (Maritime Antarctica) as determined by Next-Generation Sequencing. Frontiers in microbiology, 10, 908.