元數據

Microorganisms (Bacteria, Archaea and phototroph eukaryotes) from Fildes Bay, King George Island, Antarctica

最新版本 由 SCAR - Microbial Antarctic Resource System 發佈於 2019年3月19日 SCAR - Microbial Antarctic Resource System
發布日期:
2019年3月19日
授權條款:
CC-BY 4.0

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說明

Amplicon sequencing dataset (Illumina MiSeq and 454 pyrosequencing) of Bacteria and Archaea (16S ssu rRNA gene) and phototroph eukaryotes (16S chloroplast) from sea water in Fildes Bay, King George Island, Antarctica

版本

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如何引用

研究者應依照以下指示引用此資源。:

Moreno-Pino M, De la Iglesia R, Valdivia N, Hendriquez-Castilo C, Galan A, Diez B, Trefault N (2019): Microorganisms (Bacteria, Archaea and phototroph eukaryotes) from Fildes Bay, King George Island, Antarctica. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbes_fildes_bay_antarctica&v=1.1

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF 註冊

此資源已向GBIF註冊,並指定以下之GBIF UUID: 9fe229a7-9015-40a7-a212-e63ca57f9828。  SCAR - Microbial Antarctic Resource System 發佈此資源,並經由Scientific Committee on Antarctic Research同意向GBIF註冊成為資料發佈者。

關鍵字

Metadata

聯絡資訊

資源建立者:
-

Mario Moreno-Pino
Universidad Mayor
Santiago
CL
Rodrigo De la Iglesia
Pontificia Universidad Catolica de Chile
Santiago
CL
Nelson Valdivia
Universidad Australe de Chile
Valdivia
CL
Carlos Hendriquez-Castilo
Pontificia Universidad Catolica de Chile
Santiago
CL
Alexander Galan
Universidad Catolica de la Santisima Concepcion
Talcahuano
CL
Beatriz Diez
Universidad Catolica de Chile
Santiago
CL
Nicole Trefault
Universidad Mayor
Santiago
CL

可回覆此資源相關問題者:

Mario Moreno-Pino
Universidad Mayor
Santiago
CL
Nicole Trefault
Universidad Mayor
Santiago
CL

元數據填寫者:
-

Maxime Sweetlove
Research assistent
Royal Belgian Institute of Natural Sciences
Rue Vautier 29
1000 Brussels
BE

與此資源的相關者:

使用者

地理涵蓋範圍

Fildes Bay, King George Island, Antarctica

界定座標範圍 緯度南界 經度西界 [-62.29, -58.94], 緯度北界 經度東界 [-62.16, -58.73]

分類群涵蓋範圍

Bacteria and Archaea (16S ssu rRNA gene)

Domain Bacteria (Bacteria), Archaea (Archaea)

phototrophic eukaryotes, based on the chloroplast 16S ssu rRNA gene

Domain Eukaryota (algae)

取樣方法

Surface water samples (5 m depth) were collected with 5 L Niskin bottles from 17 different locations. Each location was assigned to one of the following four categories: Collins Glacier (‘C’ stations), Nelson Glacier (‘N’ stations), Fildes Bay (‘F’ stations) and Inner Bay (‘IB’ stations). Seawater samples (5 L) were prefiltered on board through 100 μm pore mesh and stored in acid-washed carboys and kept on dark until subsampling at the laboratory.

研究範圍 Water samples were taken at Fildes Bay, King George Island, Antarctica, on 7 and 8 February 2012

方法步驟描述:

  1. For photosynthetic eukaryote identification, plastidial 16S rRNA PCR products (in triplicates) were obtained using primer pair PLA491F–OXY1313, purified using Zymoclean kit (Zymo Research) and checked on an Agilent Bioanalzyer DNA1000 chip for the absence of primer dimers, and quantified using a PicoGreen dsDNA quantitation reagent (Invitrogen). Equal amount of purified PCR products were pooled for subsequent 454 pyrosequencing using a Roche GS-FLX Junior.
  2. For bacterial identification, general 16S rRNA PCR products (in triplicates) were obtained using primers 515Fseq and 806rcbc (Caporaso et al.2011) following conditions from Earth Microbiome Project (EMP) (Gilbert, Jansson and Knight 2014). Illumina primer constructs were obtained from EMP also. Amplicons were quantified using KAPA Library Quantification Kit (KAPA Biosystem) and sequenced using Illumina Miseq following Caporaso et al. (2012) protocol. 12 pM of qPCR quantified amplicons pool were sequenced using a 300 cycles Illumina Miseq kit.

引用文獻

  1. Moreno-Pino, M., De la Iglesia, R., Valdivia, N., Henríquez-Castilo, C., Galán, A., Díez, B., & Trefault, N. (2016). Variation in coastal Antarctic microbial community composition at sub-mesoscale: spatial distance or environmental filtering?. FEMS microbiology ecology, 92(7). doi: 10.1093/femsec/ w088

額外的詮釋資料

替代的識別碼 9fe229a7-9015-40a7-a212-e63ca57f9828
https://ipt.biodiversity.aq/resource?r=microbes_fildes_bay_antarctica