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Microorganisms (Bacteria, Archaea and phototroph eukaryotes) from Fildes Bay, King George Island, Antarctica

Latest version published by SCAR - Microbial Antarctic Resource System on Mar 19, 2019 SCAR - Microbial Antarctic Resource System

Amplicon sequencing dataset (Illumina MiSeq and 454 pyrosequencing) of Bacteria and Archaea (16S ssu rRNA gene) and phototroph eukaryotes (16S chloroplast) from sea water in Fildes Bay, King George Island, Antarctica

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How to cite

Researchers should cite this work as follows:

Moreno-Pino M, De la Iglesia R, Valdivia N, Hendriquez-Castilo C, Galan A, Diez B, Trefault N (2019): Microorganisms (Bacteria, Archaea and phototroph eukaryotes) from Fildes Bay, King George Island, Antarctica. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbes_fildes_bay_antarctica&v=1.1

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 9fe229a7-9015-40a7-a212-e63ca57f9828.  SCAR - Microbial Antarctic Resource System publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Scientific Committee on Antarctic Research.

Keywords

Metadata

Contacts

Who created the resource:

Mario Moreno-Pino
Universidad Mayor Santiago CL
Rodrigo De la Iglesia
Pontificia Universidad Catolica de Chile Santiago CL
Nelson Valdivia
Universidad Australe de Chile Valdivia CL
Carlos Hendriquez-Castilo
Pontificia Universidad Catolica de Chile Santiago CL
Alexander Galan
Universidad Catolica de la Santisima Concepcion Talcahuano CL
Beatriz Diez
Universidad Catolica de Chile Santiago CL
Nicole Trefault
Universidad Mayor Santiago CL

Who can answer questions about the resource:

Mario Moreno-Pino
Universidad Mayor Santiago CL
Nicole Trefault
Universidad Mayor Santiago CL

Who filled in the metadata:

Maxime Sweetlove
Research assistent
Royal Belgian Institute of Natural Sciences Rue Vautier 29 1000 Brussels BE

Who else was associated with the resource:

User

Geographic Coverage

Fildes Bay, King George Island, Antarctica

Bounding Coordinates South West [-62.29, -58.94], North East [-62.16, -58.73]

Taxonomic Coverage

Bacteria and Archaea (16S ssu rRNA gene)

Domain  Bacteria (Bacteria),  Archaea (Archaea)

phototrophic eukaryotes, based on the chloroplast 16S ssu rRNA gene

Domain  Eukaryota (algae)

Sampling Methods

Surface water samples (5 m depth) were collected with 5 L Niskin bottles from 17 different locations. Each location was assigned to one of the following four categories: Collins Glacier (‘C’ stations), Nelson Glacier (‘N’ stations), Fildes Bay (‘F’ stations) and Inner Bay (‘IB’ stations). Seawater samples (5 L) were prefiltered on board through 100 μm pore mesh and stored in acid-washed carboys and kept on dark until subsampling at the laboratory.

Study Extent Water samples were taken at Fildes Bay, King George Island, Antarctica, on 7 and 8 February 2012

Method step description:

  1. For photosynthetic eukaryote identification, plastidial 16S rRNA PCR products (in triplicates) were obtained using primer pair PLA491F–OXY1313, purified using Zymoclean kit (Zymo Research) and checked on an Agilent Bioanalzyer DNA1000 chip for the absence of primer dimers, and quantified using a PicoGreen dsDNA quantitation reagent (Invitrogen). Equal amount of purified PCR products were pooled for subsequent 454 pyrosequencing using a Roche GS-FLX Junior.
  2. For bacterial identification, general 16S rRNA PCR products (in triplicates) were obtained using primers 515Fseq and 806rcbc (Caporaso et al.2011) following conditions from Earth Microbiome Project (EMP) (Gilbert, Jansson and Knight 2014). Illumina primer constructs were obtained from EMP also. Amplicons were quantified using KAPA Library Quantification Kit (KAPA Biosystem) and sequenced using Illumina Miseq following Caporaso et al. (2012) protocol. 12 pM of qPCR quantified amplicons pool were sequenced using a 300 cycles Illumina Miseq kit.

Bibliographic Citations

  1. Moreno-Pino, M., De la Iglesia, R., Valdivia, N., Henríquez-Castilo, C., Galán, A., Díez, B., & Trefault, N. (2016). Variation in coastal Antarctic microbial community composition at sub-mesoscale: spatial distance or environmental filtering?. FEMS microbiology ecology, 92(7). doi: 10.1093/femsec/ w088

Additional Metadata

Alternative Identifiers 9fe229a7-9015-40a7-a212-e63ca57f9828
https://ipt.biodiversity.aq/resource?r=microbes_fildes_bay_antarctica