Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition

元數據
最新版本 published by SCAR - Microbial Antarctic Resource System on 六月 8, 2021 SCAR - Microbial Antarctic Resource System
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2021年6月8日
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CC-BY 4.0

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說明

Amplicon sequencing dataset targeting eukaryote microorganisms (18S ssu rRNA marker gene) in permanently ice-covered lakes in the McMurdo Dry Valleys (Antarctica). Samples (n=89) were taken in the photic zone of the east lobe (ELB) and west lobe (WLB) of the chemically stratified Lake Bonney.

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如何引用

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Bielewicz S, Bell E, Kong W, Froedberg I, Priscu J, Morgan-Kiss R (2021): Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=protist_diversity_in_antarctic_lake_during_polar_night&v=1.1

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此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

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關鍵字

Metadata

聯絡資訊

Scott Bielewicz
  • 出處
Miami University
Oxford
US
Elanor Bell
  • 出處
Scottish Marine Institute
Argyll
GB
Weidong Kong
  • 出處
Miami University
Oxford
US
Iddo Froedberg
  • 出處
Miami University
Oxford
US
John Priscu
  • 出處
Montana State University
Bozeman
US
Rachael Morgan-Kiss
  • 出處 ●
  • 使用者 ●
  • 連絡人
Miami University
Oxford
US
Maxime Sweetlove
  • 元數據提供者
Royal Belgian Institute of Natural Sciences
Brussels
BE

地理涵蓋範圍

Lake Bonney, McMurdo Dry Valleys, Antarctica West = East =

界定座標範圍 緯度南界 經度西界 [-77.435, 161.405], 緯度北界 經度東界 [-77.316, 163.743]

時間涵蓋範圍

起始日期 / 結束日期 2010-11-18 / 2011-03-15

計畫資料

Mc Murdo Dry Valleys Long Term Ecological Research Project (LTER; http://www.mcmlter.org/)

計畫名稱 Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition
經費來源 National Science Foundation (NSF)

參與計畫的人員:

Rachael Morgan-Kiss

取樣方法

Samples (1 liter) were vacuum concentrated (5 kPa) onto 47 mm 0.45 µm Durapore polyvinylidene fluoride membrane filters (Millipore). The filters were frozen immediately in liquid nitrogen and transported frozen to McMurdo Station, where they were stored at -80 degrees C until DNA extraction.

研究範圍 Lake Bonney in the McMurdo Dry Valleys (Antarctica). Samples were collected at 6, 13, 18, and 20m in the east lobe (ELB) and 10, 13, 15, and 20m in the west lobe (WLB) of Lake Bonney from February to April 2008. All collection depths are relative to the piezometric water level in the ice borehole.

方法步驟描述:

  1. DNA was isolated from a whole filter using the MP DNA kit (MP Biomedicals, CA) following the kit’s instruction. Sequencing was performed using the BigDye Terminator v3.1 cycle sequencing kit (ABI, CA) with M13R primer and the fragments were sequenced on an Applied Biosystems 3730×l DNA Analyzer (ABI, CA). The 18S rRNA gene sequences reported in the current study have been deposited in GenBank under accession numbers GU969060 to GU969102.

引用文獻

  1. Bielewicz, S., Bell, E., Kong, W., Friedberg, I., Priscu, J. C., & Morgan-Kiss, R. M. (2011). Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition. The ISME Journal, 5(9), 1559–1564. doi:10.1038/ismej.2011.23