Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition

Latest version published by SCAR - Microbial Antarctic Resource System on Jun 8, 2021 SCAR - Microbial Antarctic Resource System

Amplicon sequencing dataset targeting eukaryote microorganisms (18S ssu rRNA marker gene) in permanently ice-covered lakes in the McMurdo Dry Valleys (Antarctica). Samples (n=89) were taken in the photic zone of the east lobe (ELB) and west lobe (WLB) of the chemically stratified Lake Bonney.

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Researchers should cite this work as follows:

Bielewicz S, Bell E, Kong W, Froedberg I, Priscu J, Morgan-Kiss R (2021): Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=protist_diversity_in_antarctic_lake_during_polar_night&v=1.1

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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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This resource has been registered with GBIF, and assigned the following GBIF UUID: 8d83b6ed-c88d-4bf6-90a2-c6ade46fee16.  SCAR - Microbial Antarctic Resource System publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Scientific Committee on Antarctic Research.

Keywords

Metadata

Contacts

Who created the resource:

Scott Bielewicz
Miami University Oxford US
Elanor Bell
Scottish Marine Institute Argyll GB
Weidong Kong
Miami University Oxford US
Iddo Froedberg
Miami University Oxford US
John Priscu
Montana State University Bozeman US
Rachael Morgan-Kiss
Miami University Oxford US

Who can answer questions about the resource:

Rachael Morgan-Kiss
Miami University Oxford US

Who filled in the metadata:

Maxime Sweetlove
Royal Belgian Institute of Natural Sciences Brussels BE

Who else was associated with the resource:

User
Rachael Morgan-Kiss
Miami University Oxford US

Geographic Coverage

Lake Bonney, McMurdo Dry Valleys, Antarctica West = East =

Bounding Coordinates South West [-77.435, 161.405], North East [-77.316, 163.743]

Temporal Coverage

Start Date / End Date 2010-11-18 / 2011-03-15

Project Data

Mc Murdo Dry Valleys Long Term Ecological Research Project (LTER; http://www.mcmlter.org/)

Title Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition
Funding National Science Foundation (NSF)

The personnel involved in the project:

Rachael Morgan-Kiss

Sampling Methods

Samples (1 liter) were vacuum concentrated (5 kPa) onto 47 mm 0.45 µm Durapore polyvinylidene fluoride membrane filters (Millipore). The filters were frozen immediately in liquid nitrogen and transported frozen to McMurdo Station, where they were stored at -80 degrees C until DNA extraction.

Study Extent Lake Bonney in the McMurdo Dry Valleys (Antarctica). Samples were collected at 6, 13, 18, and 20m in the east lobe (ELB) and 10, 13, 15, and 20m in the west lobe (WLB) of Lake Bonney from February to April 2008. All collection depths are relative to the piezometric water level in the ice borehole.

Method step description:

  1. DNA was isolated from a whole filter using the MP DNA kit (MP Biomedicals, CA) following the kit’s instruction. Sequencing was performed using the BigDye Terminator v3.1 cycle sequencing kit (ABI, CA) with M13R primer and the fragments were sequenced on an Applied Biosystems 3730×l DNA Analyzer (ABI, CA). The 18S rRNA gene sequences reported in the current study have been deposited in GenBank under accession numbers GU969060 to GU969102.

Bibliographic Citations

  1. Bielewicz, S., Bell, E., Kong, W., Friedberg, I., Priscu, J. C., & Morgan-Kiss, R. M. (2011). Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition. The ISME Journal, 5(9), 1559–1564. doi:10.1038/ismej.2011.23