Global biogeography of desert cyanobacteria

Последняя версия опубликована SCAR - Microbial Antarctic Resource System Mar 19, 2019 SCAR - Microbial Antarctic Resource System

Amplicon sequencing dataset (454 pyrosequencing) of Bacteria (16S ssu rRNA) and Cyanobacteria (nifH) in cold desert quartz rocks

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Как оформить ссылку

Исследователи должны дать ссылку на эту работу следующим образом:

Lacap-bugler D, Lee K, Archer S, Gillman L, Lau M, Leuzinger S, Lee C, Maki T, McKay C, Perrott J, de los Rios-Murillo A, Warren-Rhodes K, Hopkins D, Pointing S (2018): Global biogeography of desert cyanobacteria. v1.4. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=desert_cyanobacteria&v=1.4

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Исследователи должны соблюдать следующие права:

Публикующей организацией и владельцем прав на данную работу является SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

Регистрация в GBIF

Этот ресурс был зарегистрирован в GBIF, ему был присвоен следующий UUID: 914f39c7-7bb2-4e77-a4ae-36147aa07daf.  SCAR - Microbial Antarctic Resource System отвечает за публикацию этого ресурса, и зарегистрирован в GBIF как издатель данных при оподдержке Scientific Committee on Antarctic Research.

Ключевые слова

Metadata

Контакты

Кто является создателем ресурса:

Donnabella Lacap-bugler
Auckland University of Technology Auckland NZ
Kevin Lee
Auckland University of Technology Auckland NZ
Stephen Archer
Auckland University of Technology Auckland NZ
Len Gillman
Auckland University of Technology Auckland NZ
Maggie Lau
Princeton University Princeton US
Sebastian Leuzinger
Auckland University of Technology Auckland NZ
Charles Lee
University of Waikato Hamilton NZ
Teruya Maki
Kanazawa University Kanazawa JP
Christoffer McKay
National Aeronautics and Space Administration Ames Research Center Moffett Field US
John Perrott
Auckland University of Technology Auckland NZ
Asunción de los Rios-Murillo
Museo Nacional de Ciencias Naturales Madrid ES
Kimberley Warren-Rhodes
Kanazawa University Kanazawa JP
David Hopkins
The Royal Agricultural University Cirencester GB
Stephen Pointing
Auckland University of Technology Auckland NZ

Кто может ответить на вопросы о ресурсе:

Donnabella Lacap-bugler
Auckland University of Technology Auckland NZ

Кем заполнены метаданные:

Maxime Sweetlove
Research assistent
Royal Belgian Institute for Natural Sciences Rue Vautier 29 1000 Brussels

Кто еще связан с данным ресурсом:

User

Географический охват

Tibetan Plateau, China; Taklimakan Desert, China; Devon Island (Arctic), Canada; McMurdo Dry Valleys, Antarctica

Ограничивающие координаты Юг Запад [-77.41, 88.616], Север Восток [42.715, 161.193]

Таксономический охват

Bacteria (16S ssu rRNA) and Cyanobacteria (nifH)

Domain  Bacteria (Bacteria)
Phylum  Cyanobacteria (Cyanobacteria)

Данные проекта

Описание отсутсвует

Название Global biogeography of desert cyanobacteria
Финансирование The research was funded by the NASA Astrobiology Science and Technology for Exploring Planets (ASTEP) program and the Institute for Applied Ecology New Zealand (www.aenz.aut.ac.nz).

Исполнители проекта:

Donnabella Lacab-bugler

Методы сбора

Colonized quartz stones were retrieved by hand (using isopropyl alcohol surface sterilized latex gloves) and loose soil particles gently removed with a sterile (autoclaved) paintbrush. Samples were then stored in sterile Whirlpak bags (Nasco) at -20°C in the field and in transit, and subsequently stored frozen at -80°C in the laboratory until processed.

Охват исследования Hypolithic communities were recovered from quartz substrate in desert pavement on the Tibetan Plateau (China), the Taklimakan Desert (China), Devon Island (Canada) and the McMurdo Dry Valleys (Antarctica).

Описание этапа методики:

  1. Amplification of 16S rRNA genes was achieved using primer pair 341F and 907R with PCR conditions involving an initial denaturation time of 5min; 30 cycles at 95°C for 1 min, 55°C for 1 min, 72°C for 1 min, and a final extension at 72°C for 10 min. Positive and negative controls were run for every PCR. For each amplicon library purification was carried out with Agencourt AMPure XP Bead (Beckman Coulter, CA, USA) according to manufacturer’s instructions. The libraries were quantified with Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen Life Technologies, NY, USA) using FLUOstar OPTIMA F fluorometer (BMG Labtech GmbH, Offenburg, Germany) and library quality was assessed with the FlashGel System (Lonza Group Ltd., Basel, Switzerland).
  2. Emulsion-PCR was carried out with GS Junior Titanium emPCR Kit (Lib-L, 454 Life Sciences Corp., CT, USA) according to the emPCR Amplification Method Manual – Lib-L, Single-Prep. The sequencing reaction was carried out with the GS Junior Titanium Sequencing Kit and GS Junior Titanium PicoTiterPlate Kit (454 Life Sciences Corp.) according to the manufacturer’s instructions. The sequencing run was conducted in 200 cycles.

Библиографические ссылки

  1. Lacap-Bugler, D. C., Lee, K. K., Archer, S., Gillman, L. N., Lau, M. C., Leuzinger, S., ... & de los Rios-Murillo, A. (2017). Global diversity of desert hypolithic cyanobacteria. Frontiers in microbiology, 8, 867.

Дополнительные метаданные

Альтернативные идентификаторы 914f39c7-7bb2-4e77-a4ae-36147aa07daf
https://ipt.biodiversity.aq/resource?r=desert_cyanobacteria