Carotenoid producing bacteria from Antarctica

Registros de Dados

Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 30 registros.

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versões

A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.

Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Vila E, Hornero-Mendez D, Azziz G, Lareo C, Saravia V (2021): Carotenoid producing bacteria from Antarctica. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Occurrence. https://ipt.biodiversity.aq/resource?r=carotenoid_bacteria_antarctica&v=1.0

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: d5010a6c-152b-4565-b799-cc8eb0e7a77b.  SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.

Palavras-chave

Occurrence

Contatos

Cobertura Geográfica

Antarctica:King George Island:Fildes Peninsula

Dados Sobre o Projeto

Nenhuma descrição disponível

O pessoal envolvido no projeto:

Métodos de Amostragem

A total of 32 liquid and solid samples were collected in 50 mL sterile tubes.

Descrição dos passos do método:

1. Approximately 100 mg of each sample was suspended in 900 μl of sterile NaCl 0.9% (w/v) solution, serially diluted and plated on adequate media. The isolation medium for samples of organic matter, sediments and ice water was Tryptic Soy Agar (TSA, Sigma Aldrich), and for sea water was TSA complemented with 20 g/L of sea salts (Sigma). Plates were incubated at 10 °C for 7–10 days, and colored colonies were selected for strain isolation by streak-plating technique. Once purity was verified, strains were conserved at −80 °C on glass beads with 20% glycerol in Tryptic Soy Broth (TSB, Oxoid) and sea salts when needed. Strains were characterized by colony and cell morphology, Gram staining and pigment composition.
2. Genomic DNA extraction was performed with a commercial kit according manufacturer’s instructions (Genomic DNA Purification Kit, Thermo Fisher). Amplification of the 16S rRNA gene fragments were done in a Palm-1870 Cycler TM (Corbett Research UK Ltd) as follows: initial denaturation 3 min at 95 °C, then 35 cycles of 45 s at 94 °C, 45 s at 58 °C, 60 s 72 °C, and a final extension step 9 min at 72 °C. Reaction mixtures contained: 2.5 U polymerase (Mango Taq, Bioline), 10 μL of buffer solution, 2.5 μL of 50 mM MgCl2, and 2.5 μL each of forward primer 27 F (5´-AGAGTTTGATC MTGGCTCAG-3´) and reverse primer 1492R (5´-TACGGYTACC TTGTTACGACTT-3´), genomic DNA, and water to 50 μL final volume. The PCR products were analyzed by electrophoresis with 1% agarose gels. DNA sequencing was carried out by Macrogen Inc. (Korea) using universal primers.

Citações bibliográficas

1. Vila, E., Hornero-Méndez, D., Azziz, G., Lareo, C., & Saravia, V. (2019). Carotenoids from heterotrophic bacteria isolated from Fildes Peninsula, King George Island, Antarctica. Biotechnology Reports, 21, e00306.

Metadados Adicionais