Carotenoid producing bacteria from Antarctica

Data Records

The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 30 records.

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versions

The table below shows only published versions of the resource that are publicly accessible.

How to cite

Researchers should cite this work as follows:

Vila E, Hornero-Mendez D, Azziz G, Lareo C, Saravia V (2021): Carotenoid producing bacteria from Antarctica. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Occurrence. https://ipt.biodiversity.aq/resource?r=carotenoid_bacteria_antarctica&v=1.0

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: d5010a6c-152b-4565-b799-cc8eb0e7a77b.  SCAR - Microbial Antarctic Resource System publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Scientific Committee on Antarctic Research.

Keywords

Occurrence

Contacts

Geographic Coverage

Antarctica:King George Island:Fildes Peninsula

Project Data

No Description available

The personnel involved in the project:

Sampling Methods

A total of 32 liquid and solid samples were collected in 50 mL sterile tubes.

Method step description:

1. Approximately 100 mg of each sample was suspended in 900 μl of sterile NaCl 0.9% (w/v) solution, serially diluted and plated on adequate media. The isolation medium for samples of organic matter, sediments and ice water was Tryptic Soy Agar (TSA, Sigma Aldrich), and for sea water was TSA complemented with 20 g/L of sea salts (Sigma). Plates were incubated at 10 °C for 7–10 days, and colored colonies were selected for strain isolation by streak-plating technique. Once purity was verified, strains were conserved at −80 °C on glass beads with 20% glycerol in Tryptic Soy Broth (TSB, Oxoid) and sea salts when needed. Strains were characterized by colony and cell morphology, Gram staining and pigment composition.
2. Genomic DNA extraction was performed with a commercial kit according manufacturer’s instructions (Genomic DNA Purification Kit, Thermo Fisher). Amplification of the 16S rRNA gene fragments were done in a Palm-1870 Cycler TM (Corbett Research UK Ltd) as follows: initial denaturation 3 min at 95 °C, then 35 cycles of 45 s at 94 °C, 45 s at 58 °C, 60 s 72 °C, and a final extension step 9 min at 72 °C. Reaction mixtures contained: 2.5 U polymerase (Mango Taq, Bioline), 10 μL of buffer solution, 2.5 μL of 50 mM MgCl2, and 2.5 μL each of forward primer 27 F (5´-AGAGTTTGATC MTGGCTCAG-3´) and reverse primer 1492R (5´-TACGGYTACC TTGTTACGACTT-3´), genomic DNA, and water to 50 μL final volume. The PCR products were analyzed by electrophoresis with 1% agarose gels. DNA sequencing was carried out by Macrogen Inc. (Korea) using universal primers.

Bibliographic Citations

1. Vila, E., Hornero-Méndez, D., Azziz, G., Lareo, C., & Saravia, V. (2019). Carotenoids from heterotrophic bacteria isolated from Fildes Peninsula, King George Island, Antarctica. Biotechnology Reports, 21, e00306.

Additional Metadata