Amplicon sequencing dataset targeting eukaryote microorganisms (18S ssu rRNA marker gene) in permanently ice-covered lakes in the McMurdo Dry Valleys (Antarctica). Samples (n=89) were taken in the photic zone of the east lobe (ELB) and west lobe (WLB) of the chemically stratified Lake Bonney.
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Bielewicz S, Bell E, Kong W, Froedberg I, Priscu J, Morgan-Kiss R (2021): Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=protist_diversity_in_antarctic_lake_during_polar_night&v=1.1
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Lake Bonney, McMurdo Dry Valleys, Antarctica West = East =
|Bounding Coordinates||South West [-77.435, 161.405], North East [-77.316, 163.743]|
|Start Date / End Date||2010-11-18 / 2011-03-15|
Mc Murdo Dry Valleys Long Term Ecological Research Project (LTER; http://www.mcmlter.org/)
|Title||Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition|
|Funding||National Science Foundation (NSF)|
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Samples (1 liter) were vacuum concentrated (5 kPa) onto 47 mm 0.45 µm Durapore polyvinylidene fluoride membrane filters (Millipore). The filters were frozen immediately in liquid nitrogen and transported frozen to McMurdo Station, where they were stored at -80 degrees C until DNA extraction.
|Study Extent||Lake Bonney in the McMurdo Dry Valleys (Antarctica). Samples were collected at 6, 13, 18, and 20m in the east lobe (ELB) and 10, 13, 15, and 20m in the west lobe (WLB) of Lake Bonney from February to April 2008. All collection depths are relative to the piezometric water level in the ice borehole.|
Method step description:
- DNA was isolated from a whole filter using the MP DNA kit (MP Biomedicals, CA) following the kit’s instruction. Sequencing was performed using the BigDye Terminator v3.1 cycle sequencing kit (ABI, CA) with M13R primer and the fragments were sequenced on an Applied Biosystems 3730×l DNA Analyzer (ABI, CA). The 18S rRNA gene sequences reported in the current study have been deposited in GenBank under accession numbers GU969060 to GU969102.
- Bielewicz, S., Bell, E., Kong, W., Friedberg, I., Priscu, J. C., & Morgan-Kiss, R. M. (2011). Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition. The ISME Journal, 5(9), 1559–1564. doi:10.1038/ismej.2011.23