Description
Amplicon sequencing dataset (Illumina MiSeq 250bp PE) of soil bacteria (16S ssu rRNA gene, v3-v4) on King George Island and Deception Island (Antarctica)
Versions
The table below shows only published versions of the resource that are publicly accessible.
How to cite
Researchers should cite this work as follows:
Chua C, Yong S, Gonzalez M, Lavin P, Cheah Y, Tan G, Wong C (2019): V3-V4 16S rDNA of soil bacteria in King George Island and Deception Island. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=soil_bacteria_king_george_island_and_deception_island&v=1.1
Rights
Researchers should respect the following rights statement:
The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF Registration
This resource has been registered with GBIF, and assigned the following GBIF UUID: fca3f5da-a6ae-464d-a488-38439546056d. SCAR - Microbial Antarctic Resource System publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Scientific Committee on Antarctic Research.
Keywords
Metadata
Contacts
- Originator ●
- Point Of Contact
- Originator
- Originator
- Originator
- Originator
- Originator
- Originator ●
- Point Of Contact
- Metadata Provider
Geographic Coverage
King George Island and Deception Island, Antarctica
| Bounding Coordinates | South West [-62.99, -60.68], North East [-62.19, -58.94] |
|---|
Taxonomic Coverage
Bacteria, 16S ssu rRNA gene, v3-v4 region
| Domain | Bacteria (Bacteria) |
|---|
Project Data
No Description available
| Title | V3-V4 16S rDNA of soil bacteria in King George Island and Deception Island |
|---|---|
| Funding | his work was supported by the Ministry of Science, Technology and Innovation (MOSTI), Malaysia under the Flagship project FP0712E012. |
The personnel involved in the project:
Sampling Methods
Samples were stored at –20°C immediately after sampling.
| Study Extent | Soil samples were collected from King George Island (S62°11′35.3′′; W58°56′6.2′′) and Deception Island (S62°59′023′′; W60°40′51.7′′), Antarctica in February 2007. |
|---|
Method step description:
- Total bacterial genomic DNA was extracted from soils using the method described in Zhou et al. (1996) DNA recovery from soils of diverse composition. Appl. Environ. Microbiol., 62(2), 316–322.
- The concentration and purity of the extracted genomic DNA were measured using the NanoDropTM 2000 spectrophotometer (Thermo Scientific Inc., Waltham, MA, USA).
- The V3–V4 region of the bacterial 16S rRNA gene was amplified using locus-specific primers, S-DBact-0341-b- S-17 (5′-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT ACG GGN GGC WGC AG-3′)and S-D-Bact-0785-a-A-21 (5′-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGA CTA CHV GGG TAT CTA ATC C-3′). The 16S rDNA amplicons were subjected to paired-end sequencing on Illumina MiSeq platform using 2 × 250 bp MiSeq Reagent Kit v2 (Illumina® Inc., San Diego, California).
Bibliographic Citations
- Chua, C. Y., Yong, S. T., González, M. A., Lavin, P., Cheah, Y. K., Tan, G. Y. A., & Wong, C. M. V. L. (2018). Analysis of bacterial communities of King George and Deception Islands, Antarctica using high-throughput sequencing. CURRENT SCIENCE, 115(9), 1701.
Additional Metadata
| Alternative Identifiers | fca3f5da-a6ae-464d-a488-38439546056d |
|---|---|
| https://ipt.biodiversity.aq/resource?r=soil_bacteria_king_george_island_and_deception_island |