cyanobacterial mats of from the Byers Peninsula, Antarctica

Dernière version Publié par SCAR - Microbial Antarctic Resource System le Mar 19, 2019 SCAR - Microbial Antarctic Resource System

Cyanobacterial 16S rRNA gene sequences from cyanobacterial mats of Antarctic (Byers Peninsula) origin obtained by clone library

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L’éditeur et détenteur des droits de cette ressource est SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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Mots-clé

Metadata

Contacts

Personne ayant créé cette ressource:

Julia Kleinteich
Researcher
University of Liège Liège Lieège BE

Personne pouvant répondre aux questions sur la ressource:

Julia Kleinteich
Researcher
University of Liège Liège Lieège BE
Daniel R Dietrich
Researcher
University of Konstanz Konztanz DE

Personne ayant renseigné les métadonnées:

Julien Cigar

Couverture géographique

Byers Peninsula, Livingston Island, Antarctica

Enveloppe géographique Sud Ouest [-62.7, -61.5], Nord Est [-62.5, -61]

Couverture taxonomique

All data provided are OTUs of the 16S rRNA gene which were identified via a comparison to online databases to family or genus level. For most OTUs identification to species level was not possible

Phylum  Cyanobacteria (Cyanobacteria)

Couverture temporelle

Date de début / Date de fin 2009-02-01 / 2009-02-28

Données sur le projet

Pas de description disponible

Titre DI698/18-1 Dietrich
Financement Deutsche Forschungsgesellschaft DFG

Les personnes impliquées dans le projet:

Chercheur Principal
Daniel R Dietrich

Méthodes d'échantillonnage

Cyanobacterial samples were collected from five different sampling sites in the Antarctic Specially Protected Area (ASPA) No. 126 of Byers Peninsula, Livingston Island (62° 34' 35" to 62° 4' 35" S and 60° 54' 14" to 61° 13' 07" W), South Shetland Islands, Antarctic Peninsula, during an expedition in February 2009. Microbial mats were probed using a sterile spatula, sealed in sterile plastic bags or tubes and stored frozen (-20 °C) for DNA extraction.

Etendue de l'étude Samples were collected on Byers Peninsula (ASPA 126) in February 2009. This area is a summer snow- and ice-free area. Various microbial mats on wet soil, meltwater and seepages were sampled.
Contrôle qualité Data were published in a peer-reviewed journal DOI: 10.1038/NCLIMATE1418

Description des étapes de la méthode:

  1. After collection the samples were stored at -20°C until further processing. DNA was extracted from the samples in three replicates and combined. 16S rRNA genes were amplified using cyanobacteria specific primers (Saker et al. 2005). PCR Products were cloned using the TOPO TA Cloning Kit following the standard protocol. Two to three clones of each individual restriction fragment length polymorphism pattern were sequenced at GATC Biotech. All sequences were deposited with the GenBank database. Saker, M. L., Jungblut, A. D., Neilan, B. A., Rawn, D. F. K. & Vasconcelos, V. M. Detection of microcystin synthetase genes in health food supplements containing the freshwater cyanobacterium Aphanizomenon flos-aquae. Toxicon 46, 555?562 (2005).

Métadonnées additionnelles

Identifiants alternatifs https://ipt.biodiversity.aq/resource?r=cyano_16s