Descrição
Seven assembled metagenoms from a metagenomic sequencing (Illumina HiSeq; paired-end) sample taken during a phytoplankton bloom in the Amundsen Sea (Antarctica, Southern Ocean)
Versões
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Como citar
Pesquisadores deveriam citar esta obra da seguinte maneira:
Delmont T, Eren M, Veneis J, Post A (2019): Metagenome assembly of an Amundsen Sea (Antarctica, Southern Ocean) water sample. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=amundsen_sea_metagenome&v=1.1
Direitos
Pesquisadores devem respeitar a seguinte declaração de direitos:
O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF Registration
Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 4f0d3a6f-c91e-4db9-a2f3-6a6007e620e2. SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.
Palavras-chave
Metadata
Contatos
- Originador ●
- Ponto De Contato
- Originador
- Originador
- Originador
- Provedor Dos Metadados
- Research assistent
- Rue Vautier 29
Cobertura Geográfica
Amundsen Sea, Southern Ocean
| Coordenadas delimitadoras | Sul Oeste [-73,342, -112,401], Norte Leste [-73,342, -112,401] |
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Cobertura Temporal
| Data Inicial | 2010-12-19 |
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Dados Sobre o Projeto
Nenhuma descrição disponível
| Título | ASPIRE Microbiome |
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| Financiamento | This work was performed with financial support from NSF Antarctic Sciences awards ANT-1142095. |
O pessoal envolvido no projeto:
Métodos de Amostragem
A sample was taken from the surface water layer in the center of a bloom (073° 34′243S 112° 40′080W, chlorophyll a > 17 μg/L, temperature of −1.2°C, phosphate: 1.31 μM, nitrite: 0.02 μM, ammonium: 0.05 μM, silicate: 77.8 μM) on 19 December 2010. This sample (6 l, 10 m depth) was passed over a 20 μm mesh, collected onto a 0.2 μm Sterivex membrane filter cartridge by pressure filtration, quickly frozen in the headspace of a LN2 dewar and stored at −80°C.
| Área de Estudo | Water samples were taken with a CTD Rosette equipped during a phytoplankton bloom event in the ASP in 2010–2011. |
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Descrição dos passos do método:
- DNA extraction was performed using the Puregene kit (Gentra) after disruption of the cells with lytic enzyme coupled to proteinase K (Sinigalliano et al., 2007). DNA was quantified using a Nanodrop 2000 instrument (Thermo Fisher Scientific, Wilmington, DE).
- Metagenomic libraries were generated with the OVATION ultralow kit (NuGen) using 100 ng of DNA and 8 amplification cycles. We constructed overlapping (2X100 nt with ~40 nt of overlap) and gapped (2X108 nt with an insert size of ~600 nt) metagenomic DNA libraries using a Pippin prep electrophoresis platform to precisely select the desired length for DNA fragments to be used for sequencing on a Hiseq platform (Illumina).
Citações bibliográficas
- Delmont, T. O., Eren, A. M., Vineis, J. H., & Post, A. F. (2015). Genome reconstructions indicate the partitioning of ecological functions inside a phytoplankton bloom in the Amundsen Sea, Antarctica. Frontiers in microbiology, 6, 1090.
Metadados Adicionais
| Identificadores alternativos | 4f0d3a6f-c91e-4db9-a2f3-6a6007e620e2 |
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| https://ipt.biodiversity.aq/resource?r=amundsen_sea_metagenome |