Metagenome assembly of an Amundsen Sea (Antarctica, Southern Ocean) water sample

元數據
最新版本 published by SCAR - Microbial Antarctic Resource System on 3月 19, 2019 SCAR - Microbial Antarctic Resource System
發布日期:
2019年3月19日
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CC-BY 4.0

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說明

Seven assembled metagenoms from a metagenomic sequencing (Illumina HiSeq; paired-end) sample taken during a phytoplankton bloom in the Amundsen Sea (Antarctica, Southern Ocean)

版本

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如何引用

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Delmont T, Eren M, Veneis J, Post A (2019): Metagenome assembly of an Amundsen Sea (Antarctica, Southern Ocean) water sample. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=amundsen_sea_metagenome&v=1.1

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此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF 註冊

此資源已向GBIF註冊，並指定以下之GBIF UUID: 4f0d3a6f-c91e-4db9-a2f3-6a6007e620e2。  SCAR - Microbial Antarctic Resource System 發佈此資源，並經由Scientific Committee on Antarctic Research同意向GBIF註冊成為資料發佈者。

關鍵字

Metadata

聯絡資訊

Tom Delmont
  • 出處 ●
  • 連絡人
Josephine Bay Paul Center for Comparative Molecular Biology and Evolution
Woods Hole
US
Murat Eren
  • 出處
Josephine Bay Paul Center for Comparative Molecular Biology and Evolution
Woods Hole
US
Joseph Veneis
  • 出處
Josephine Bay Paul Center for Comparative Molecular Biology and Evolution
Woods Hole
US
Anton Post
  • 出處
University of Rhode Island
Narragansett
US
Maxime Sweetlove
  • 元數據提供者
  • Research assistent
Royal Belgian Institute of Natural Sciences
  • Rue Vautier 29
1000 Brussels
BE

地理涵蓋範圍

Amundsen Sea, Southern Ocean

界定座標範圍 緯度南界 經度西界 [-73.342, -112.401], 緯度北界 經度東界 [-73.342, -112.401]

時間涵蓋範圍

起始日期 2010-12-19

計畫資料

無相關描述

計畫名稱 ASPIRE Microbiome
經費來源 This work was performed with financial support from NSF Antarctic Sciences awards ANT-1142095.

參與計畫的人員:

Anton Post

取樣方法

A sample was taken from the surface water layer in the center of a bloom (073° 34′243S 112° 40′080W, chlorophyll a > 17 μg/L, temperature of −1.2°C, phosphate: 1.31 μM, nitrite: 0.02 μM, ammonium: 0.05 μM, silicate: 77.8 μM) on 19 December 2010. This sample (6 l, 10 m depth) was passed over a 20 μm mesh, collected onto a 0.2 μm Sterivex membrane filter cartridge by pressure filtration, quickly frozen in the headspace of a LN2 dewar and stored at −80°C.

研究範圍 Water samples were taken with a CTD Rosette equipped during a phytoplankton bloom event in the ASP in 2010–2011.

方法步驟描述:

  1. DNA extraction was performed using the Puregene kit (Gentra) after disruption of the cells with lytic enzyme coupled to proteinase K (Sinigalliano et al., 2007). DNA was quantified using a Nanodrop 2000 instrument (Thermo Fisher Scientific, Wilmington, DE).
  2. Metagenomic libraries were generated with the OVATION ultralow kit (NuGen) using 100 ng of DNA and 8 amplification cycles. We constructed overlapping (2X100 nt with ~40 nt of overlap) and gapped (2X108 nt with an insert size of ~600 nt) metagenomic DNA libraries using a Pippin prep electrophoresis platform to precisely select the desired length for DNA fragments to be used for sequencing on a Hiseq platform (Illumina).

引用文獻

  1. Delmont, T. O., Eren, A. M., Vineis, J. H., & Post, A. F. (2015). Genome reconstructions indicate the partitioning of ecological functions inside a phytoplankton bloom in the Amundsen Sea, Antarctica. Frontiers in microbiology, 6, 1090.

額外的詮釋資料

替代的識別碼 4f0d3a6f-c91e-4db9-a2f3-6a6007e620e2
https://ipt.biodiversity.aq/resource?r=amundsen_sea_metagenome