Descripción
Amplicon sequencing dataset (Illumina MiSeq) targeting Fungi (ITS) in ice samples (n=8) from the Antarctic Peninsula.
Versiones
La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.
¿Cómo referenciar?
Los usuarios deben citar este trabajo de la siguiente manera:
de Menezes G, Camara P, Pinto O, Convey P, Calvarho-Silva M, Simões J, Rosa C, Rosa L (2021): Antarctic cryosphere fungal diversity (2015). v1.8. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=cryosphere_fungi_antarctica_2015&v=1.8
Derechos
Los usuarios deben respetar los siguientes derechos de uso:
El publicador y propietario de los derechos de este trabajo es SCAR - Microbial Antarctic Resource System. Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento (CC-BY 4.0).
Registro GBIF
Este recurso ha sido registrado en GBIF con el siguiente UUID: 6c3e071e-2bd9-44e9-a4c0-5222044c1b45. SCAR - Microbial Antarctic Resource System publica este recurso y está registrado en GBIF como un publicador de datos avalado por Scientific Committee on Antarctic Research.
Palabras clave
Metadata
Contactos
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- Proveedor De Los Metadatos
Cobertura geográfica
Antarctica: maritime Antarctica.
| Coordenadas límite | Latitud Mínima Longitud Mínima [-64,75, -62,217], Latitud Máxima Longitud Máxima [-62,492, -59,633] |
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Cobertura temporal
| Fecha Inicial / Fecha Final | 2015-12-01 / 2016-12-31 |
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Datos del proyecto
No hay descripción disponible
| Título | Antarctic cryosphere fungal diversity (2015) |
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| Fuentes de Financiación | This project was funded by PROANTAR CNPq (442258/2018-6), INCT Criosfera, FAPEMIG, CAPES and FNDCT. Additional support was provided by CNPq (151195/2019-6). |
Personas asociadas al proyecto:
Métodos de muestreo
Samples were collected using sterile suits and gloves. Each sample was broken into smaller pieces, and surface decontamination carried out using 5% sodium hypochlorite (10 s), sterilized distilled water (10 s), and exposure to ultraviolet radiation (10 min).
| Área de Estudio | Glacial ice fragments of approximately 20 kg mass, were collected adjacent to the ice fronts of seven marine terminating glaciers in the South Shetland Islands and the north-west Antarctica Peninsula during the austral summer season in December 2015 and December 2016. |
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Descripción de la metodología paso a paso:
- The samples were melted and a total of 12–15 L of the resulting water Filtered through 47 mm diameter (Millipore) membranes (three membranes per sampling site, each using 4–5 L) until each membrane became saturated. Membranes were then stored at -20°C until DNA extraction.
- The three membranes resulting from filtering the melted ice from each sampling site were processed together in order to increase DNA yield. Total DNA was extracted using 0.5 mL extraction buffer [sodium dodecyl sulfate (SDS) 10%], left at 55°C for 18 h, followed by 165 μL NaCl (5 M) and 165 μL cetyltrimethylammonium bromide (CTAB, 10%), then 600 μL chloroform was added and the mixture centrifuged (Eppendorf/Germany) at 13,000 rpm for 10 min. The supernatant was cleaned using the QIAGEN DNeasy PowerClean cleanup Kit. The ITS2 region was used as a DNA barcode, using the universal primers ITS3 and ITS4 and were sequenced at Macrogen Inc. (South Korea) on an Illumina MiSeq sequencer, using the MiSeq Reagent Kit v3 (600-cycle) following the manufacturer’s protocol.
Referencias bibliográficas
- de Menezes, G., Câmara, P., Pinto, O., Convey, P., Carvalho-Silva, M., Simões, J., ... & Rosa, L. (2021). Fungi in the Antarctic cryosphere: using DNA metabarcoding to reveal fungal diversity in glacial ice from the Antarctic Peninsula region.
Metadatos adicionales
| Identificadores alternativos | https://ipt.biodiversity.aq/resource?r=cryosphere_fungi_antarctica_2015 |
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