Antarctic cryosphere fungal diversity (2015)
Latest version published by SCAR - Microbial Antarctic Resource System on 17 June 2021 SCAR - Microbial Antarctic Resource System

Amplicon sequencing dataset (Illumina MiSeq) targeting Fungi (ITS) in ice samples (n=8) from the Antarctic Peninsula.

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Researchers should cite this work as follows:

de Menezes G, Camara P, Pinto O, Convey P, Calvarho-Silva M, Simões J, Rosa C, Rosa L (2021): Antarctic cryosphere fungal diversity (2015). v1.8. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=cryosphere_fungi_antarctica_2015&v=1.8

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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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This resource has been registered with GBIF, and assigned the following GBIF UUID: 6c3e071e-2bd9-44e9-a4c0-5222044c1b45.  SCAR - Microbial Antarctic Resource System publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Scientific Committee on Antarctic Research.

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Contacts

Who created the resource:

Graciele de Menezes
Universidade Federal de Minas
BR
Paulo Camara
Universidade de Brasília
BR
Otavio Pinto
Universidade de Brasília
BR
Peter Convey
British Antarctic Survey
GB
Micheline Calvarho-Silva
Universidade de Brasília
BR
Jefferson Simões
Universidade Federal do Rio Grande do Sul
BR
Carlos Rosa
Universidade Federal de Minas
BR
Luiz Rosa
Universidade Federal de Minas
BR

Who can answer questions about the resource:

Luiz Rosa
Universidade Federal de Minas
BR

Who filled in the metadata:

Maxime Sweetlove
Royal Belgian Institute of Natural Sciences
Brussels
BE

Who else was associated with the resource:

User
Luiz Rosa
Universidade Federal de Minas
BR
Geographic Coverage

Antarctica: maritime Antarctica.

Bounding Coordinates South West [-64.75, -62.217], North East [-62.492, -59.633]
Temporal Coverage
Start Date / End Date 2015-12-01 / 2016-12-31
Project Data

No Description available

Title Antarctic cryosphere fungal diversity (2015)
Funding This project was funded by PROANTAR CNPq (442258/2018-6), INCT Criosfera, FAPEMIG, CAPES and FNDCT. Additional support was provided by CNPq (151195/2019-6).

The personnel involved in the project:

Luiz Rosa
Sampling Methods

Samples were collected using sterile suits and gloves. Each sample was broken into smaller pieces, and surface decontamination carried out using 5% sodium hypochlorite (10 s), sterilized distilled water (10 s), and exposure to ultraviolet radiation (10 min).

Study Extent Glacial ice fragments of approximately 20 kg mass, were collected adjacent to the ice fronts of seven marine terminating glaciers in the South Shetland Islands and the north-west Antarctica Peninsula during the austral summer season in December 2015 and December 2016.

Method step description:

  1. The samples were melted and a total of 12–15 L of the resulting water Filtered through 47 mm diameter (Millipore) membranes (three membranes per sampling site, each using 4–5 L) until each membrane became saturated. Membranes were then stored at -20°C until DNA extraction.
  2. The three membranes resulting from filtering the melted ice from each sampling site were processed together in order to increase DNA yield. Total DNA was extracted using 0.5 mL extraction buffer [sodium dodecyl sulfate (SDS) 10%], left at 55°C for 18 h, followed by 165 μL NaCl (5 M) and 165 μL cetyltrimethylammonium bromide (CTAB, 10%), then 600 μL chloroform was added and the mixture centrifuged (Eppendorf/Germany) at 13,000 rpm for 10 min. The supernatant was cleaned using the QIAGEN DNeasy PowerClean cleanup Kit. The ITS2 region was used as a DNA barcode, using the universal primers ITS3 and ITS4 and were sequenced at Macrogen Inc. (South Korea) on an Illumina MiSeq sequencer, using the MiSeq Reagent Kit v3 (600-cycle) following the manufacturer’s protocol.
Bibliographic Citations
  1. de Menezes, G., Câmara, P., Pinto, O., Convey, P., Carvalho-Silva, M., Simões, J., ... & Rosa, L. (2021). Fungi in the Antarctic cryosphere: using DNA metabarcoding to reveal fungal diversity in glacial ice from the Antarctic Peninsula region.
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