Antarctic cryosphere fungal diversity (2015)
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de Menezes G, Camara P, Pinto O, Convey P, Calvarho-Silva M, Simões J, Rosa C, Rosa L (2021): Antarctic cryosphere fungal diversity (2015). v1.8. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=cryosphere_fungi_antarctica_2015&v=1.8
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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
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Antarctica: maritime Antarctica.
|Bounding Coordinates||South West [-64.75, -62.217], North East [-62.492, -59.633]|
|Start Date / End Date||2015-12-01 / 2016-12-31|
No Description available
|Title||Antarctic cryosphere fungal diversity (2015)|
|Funding||This project was funded by PROANTAR CNPq (442258/2018-6), INCT Criosfera, FAPEMIG, CAPES and FNDCT. Additional support was provided by CNPq (151195/2019-6).|
The personnel involved in the project:
Samples were collected using sterile suits and gloves. Each sample was broken into smaller pieces, and surface decontamination carried out using 5% sodium hypochlorite (10 s), sterilized distilled water (10 s), and exposure to ultraviolet radiation (10 min).
|Study Extent||Glacial ice fragments of approximately 20 kg mass, were collected adjacent to the ice fronts of seven marine terminating glaciers in the South Shetland Islands and the north-west Antarctica Peninsula during the austral summer season in December 2015 and December 2016.|
Method step description:
- The samples were melted and a total of 12–15 L of the resulting water Filtered through 47 mm diameter (Millipore) membranes (three membranes per sampling site, each using 4–5 L) until each membrane became saturated. Membranes were then stored at -20°C until DNA extraction.
- The three membranes resulting from filtering the melted ice from each sampling site were processed together in order to increase DNA yield. Total DNA was extracted using 0.5 mL extraction buffer [sodium dodecyl sulfate (SDS) 10%], left at 55°C for 18 h, followed by 165 μL NaCl (5 M) and 165 μL cetyltrimethylammonium bromide (CTAB, 10%), then 600 μL chloroform was added and the mixture centrifuged (Eppendorf/Germany) at 13,000 rpm for 10 min. The supernatant was cleaned using the QIAGEN DNeasy PowerClean cleanup Kit. The ITS2 region was used as a DNA barcode, using the universal primers ITS3 and ITS4 and were sequenced at Macrogen Inc. (South Korea) on an Illumina MiSeq sequencer, using the MiSeq Reagent Kit v3 (600-cycle) following the manufacturer’s protocol.
- de Menezes, G., Câmara, P., Pinto, O., Convey, P., Carvalho-Silva, M., Simões, J., ... & Rosa, L. (2021). Fungi in the Antarctic cryosphere: using DNA metabarcoding to reveal fungal diversity in glacial ice from the Antarctic Peninsula region.