metadata

ITS samples from the air and snow of Livingston Island

Última versión Publicado por SCAR - Microbial Antarctic Resource System en 7 de junio de 2021 SCAR - Microbial Antarctic Resource System
Snow (n=2) and air (n=1) samples from Livingston Island (Antarctica) were analyzed for the presence of Fungi using amplicon sequencing techniques (Illumina MiSeq, ITS2 marker gene).
Fecha de publicación:
7 de junio de 2021
Licencia:
CC-BY 4.0

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Versiones

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¿Cómo referenciar?

Los usuarios deben citar este trabajo de la siguiente manera:

Rosa L H, Pinto O H B, Santl-Temkiv T, Convey P, Carvalho-Silva M, Rosa C A, Camara P (2021): ITS samples from the air and snow of Livingston Island. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=fungi_snow_and_air_livingston_island&v=1.0

Derechos

Los usuarios deben respetar los siguientes derechos de uso:

El publicador y propietario de los derechos de este trabajo es SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

Registro GBIF

Este recurso ha sido registrado en GBIF con el siguiente UUID: 1710f7e0-3e36-4243-be38-9f11f18f30ea.  SCAR - Microbial Antarctic Resource System publica este recurso, y está registrado en GBIF como un publicador de datos avalado por Scientific Committee on Antarctic Research.

Palabras clave

Metadata

Contactos

¿Quién creó el recurso?:

Luis Hendrique Rosa
Universidade Federal de Minas Gerais
BR
Otavo Henrique Bezzera Pinto
BR
Tina Santl-Temkiv
DK
Peter Convey
GB
Micheline Carvalho-Silva
BR
Carlos Augusto Rosa
BR
Paulo Camara
BR

¿Quién puede resolver dudas acerca del recurso?:

Luiz Hendrique Rosa
Universidade Federal de Minas Gerais
BR

¿Quién documentó los metadatos?:

Maxime Sweetlove
Royal Belgian Institute of Natural Sciences
Brussels
BE

Cobertura geográfica

Antarctica: Livingston Island, near Spanish Juan Carlos I station

Coordenadas límite Latitud Mínima Longitud Mínima [-62,671, -60,379], Latitud Máxima Longitud Máxima [-62,671, -60,379]

Cobertura temporal

Fecha Inicial / Fecha Final 2019-03-11 / 2019-03-20

Datos del proyecto

No hay descripción disponible

Título ITS samples from the air and snow of Livingston Island
Fuentes de Financiación This study received financial support from CNPq, PROANTAR, FAPEMIG, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES), and INCT Criosfera 2. NERC core funding to the British Antarctic Survey’s ‘Biodiversity, Evolution and Adaptation’ Team also provided support, as well as the Spanish Polar Committee and its staff at Gabriel de Castilla base.

Personas asociadas al proyecto:

Luiz Hendrique Rosa

Métodos de muestreo

Two air samples were collected with a high flow glass impinger following Šantl-Temkiv et al. The chamber was filled with 2 L of sampling liquid (ddH2O) and the sampler was run for 5 min, so that the liquid came in contact with the entire chamber, after which 0.5 L of the sampling liquid was removed, stored as a control, and analyzed along with the samples. The resulting solution was filtered directly on the Sterivex filter units for the air. Air was collected over c. 5 h on March 11th 2019. In addition, the two separate air DNA extractions were combined together in order to increase DNA yield. Two freshly deposited snow samples were collected on March 20th 2019 at the same site using a sterilized shovel. Both pairs of samples were separately combined in order to increase DNA yield. Snow was melted at room temperature, under strictly sterile conditions, for 24 h in the laboratory at Juan Carlos I Station and then filtered using Sterivex filters.

Área de Estudio Air and snow samples were collected at Punta Polaca (62°40′16″ S; 60°22′43″ W), Hurd Peninsula, Livingston Island, South Shetland Islands, near to the Spanish station Juan Carlos I in March 2019.
Control de Calidad The control represented a field blank to certify that the samples were not contaminated by external organisms.

Descripción de la metodología paso a paso:

  1. Total DNA was extracted from environmental samples using the Qiagen Power Soil Kit (Qiagen, USA) following the manufacturer’s instructions. Extracted DNA was used as template for generating PCR amplicons. The internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA was used as a DNA barcode for molecular species identification. PCR amplicons were generated using the universal primers ITS3 and ITS4 and were sequenced by high-throughput sequencing at Macrogen Inc. (South Korea) on an Illumina MiSeq sequencer, using the MiSeq Reagent Kit v3 (600-cycle) following the manufacturer’s protocol.

Referencias bibliográficas

  1. Rosa, L. H., Pinto, O. H. B., Šantl-Temkiv, T., Convey, P., Carvalho-Silva, M., Rosa, C. A., & Câmara, P. E. (2020). DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica. Scientific reports, 10(1), 1-11.

Metadatos adicionales

Propósito Raw DNA sequence data
Descripción de mantenimiento BioProject PRJNA669512
Identificadores alternativos https://ipt.biodiversity.aq/resource?r=fungi_snow_and_air_livingston_island