說明
Snow (n=2) and air (n=1) samples from Livingston Island (Antarctica) were analyzed for the presence of Fungi using amplicon sequencing techniques (Illumina MiSeq, ITS2 marker gene).
版本
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如何引用
研究者應依照以下指示引用此資源。:
Rosa L H, Pinto O H B, Santl-Temkiv T, Convey P, Carvalho-Silva M, Rosa C A, Camara P (2021): ITS samples from the air and snow of Livingston Island. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=fungi_snow_and_air_livingston_island&v=1.0
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: 1710f7e0-3e36-4243-be38-9f11f18f30ea。 SCAR - Microbial Antarctic Resource System 發佈此資源，並經由Scientific Committee on Antarctic Research同意向GBIF註冊成為資料發佈者。
關鍵字
Metadata
聯絡資訊
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- 元數據提供者
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地理涵蓋範圍
Antarctica: Livingston Island, near Spanish Juan Carlos I station
| 界定座標範圍 | 緯度南界 經度西界 [-62.671, -60.379], 緯度北界 經度東界 [-62.671, -60.379] |
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時間涵蓋範圍
| 起始日期 / 結束日期 | 2019-03-11 / 2019-03-20 |
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計畫資料
無相關描述
| 計畫名稱 | ITS samples from the air and snow of Livingston Island |
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| 經費來源 | This study received financial support from CNPq, PROANTAR, FAPEMIG, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES), and INCT Criosfera 2. NERC core funding to the British Antarctic Survey’s ‘Biodiversity, Evolution and Adaptation’ Team also provided support, as well as the Spanish Polar Committee and its staff at Gabriel de Castilla base. |
參與計畫的人員:
取樣方法
Two air samples were collected with a high flow glass impinger following Šantl-Temkiv et al. The chamber was filled with 2 L of sampling liquid (ddH2O) and the sampler was run for 5 min, so that the liquid came in contact with the entire chamber, after which 0.5 L of the sampling liquid was removed, stored as a control, and analyzed along with the samples. The resulting solution was filtered directly on the Sterivex filter units for the air. Air was collected over c. 5 h on March 11th 2019. In addition, the two separate air DNA extractions were combined together in order to increase DNA yield. Two freshly deposited snow samples were collected on March 20th 2019 at the same site using a sterilized shovel. Both pairs of samples were separately combined in order to increase DNA yield. Snow was melted at room temperature, under strictly sterile conditions, for 24 h in the laboratory at Juan Carlos I Station and then filtered using Sterivex filters.
| 研究範圍 | Air and snow samples were collected at Punta Polaca (62°40′16″ S; 60°22′43″ W), Hurd Peninsula, Livingston Island, South Shetland Islands, near to the Spanish station Juan Carlos I in March 2019. |
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| 品質控管 | The control represented a field blank to certify that the samples were not contaminated by external organisms. |
方法步驟描述:
- Total DNA was extracted from environmental samples using the Qiagen Power Soil Kit (Qiagen, USA) following the manufacturer’s instructions. Extracted DNA was used as template for generating PCR amplicons. The internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA was used as a DNA barcode for molecular species identification. PCR amplicons were generated using the universal primers ITS3 and ITS4 and were sequenced by high-throughput sequencing at Macrogen Inc. (South Korea) on an Illumina MiSeq sequencer, using the MiSeq Reagent Kit v3 (600-cycle) following the manufacturer’s protocol.
引用文獻
- Rosa, L. H., Pinto, O. H. B., Šantl-Temkiv, T., Convey, P., Carvalho-Silva, M., Rosa, C. A., & Câmara, P. E. (2020). DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica. Scientific reports, 10(1), 1-11.
額外的詮釋資料
| 目的 | Raw DNA sequence data |
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| 維護說明 | BioProject PRJNA669512 |
| 替代的識別碼 | https://ipt.biodiversity.aq/resource?r=fungi_snow_and_air_livingston_island |