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ITS samples from the air and snow of Livingston Island

Dernière version Publié par SCAR - Microbial Antarctic Resource System le 7 juin 2021 SCAR - Microbial Antarctic Resource System
Snow (n=2) and air (n=1) samples from Livingston Island (Antarctica) were analyzed for the presence of Fungi using amplicon sequencing techniques (Illumina MiSeq, ITS2 marker gene).
Date de publication:
7 juin 2021
Licence:
CC-BY 4.0

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Rosa L H, Pinto O H B, Santl-Temkiv T, Convey P, Carvalho-Silva M, Rosa C A, Camara P (2021): ITS samples from the air and snow of Livingston Island. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=fungi_snow_and_air_livingston_island&v=1.0

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L’éditeur et détenteur des droits de cette ressource est SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 1710f7e0-3e36-4243-be38-9f11f18f30ea.  SCAR - Microbial Antarctic Resource System publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du Scientific Committee on Antarctic Research.

Mots-clé

Metadata

Contacts

Personne ayant créé cette ressource:

Luis Hendrique Rosa
Universidade Federal de Minas Gerais
BR
Otavo Henrique Bezzera Pinto
BR
Tina Santl-Temkiv
DK
Peter Convey
GB
Micheline Carvalho-Silva
BR
Carlos Augusto Rosa
BR
Paulo Camara
BR

Personne pouvant répondre aux questions sur la ressource:

Luiz Hendrique Rosa
Universidade Federal de Minas Gerais
BR

Personne ayant renseigné les métadonnées:

Maxime Sweetlove
Royal Belgian Institute of Natural Sciences
Brussels
BE

Couverture géographique

Antarctica: Livingston Island, near Spanish Juan Carlos I station

Enveloppe géographique Sud Ouest [-62,671, -60,379], Nord Est [-62,671, -60,379]

Couverture temporelle

Date de début / Date de fin 2019-03-11 / 2019-03-20

Données sur le projet

Pas de description disponible

Titre ITS samples from the air and snow of Livingston Island
Financement This study received financial support from CNPq, PROANTAR, FAPEMIG, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES), and INCT Criosfera 2. NERC core funding to the British Antarctic Survey’s ‘Biodiversity, Evolution and Adaptation’ Team also provided support, as well as the Spanish Polar Committee and its staff at Gabriel de Castilla base.

Les personnes impliquées dans le projet:

Luiz Hendrique Rosa

Méthodes d'échantillonnage

Two air samples were collected with a high flow glass impinger following Šantl-Temkiv et al. The chamber was filled with 2 L of sampling liquid (ddH2O) and the sampler was run for 5 min, so that the liquid came in contact with the entire chamber, after which 0.5 L of the sampling liquid was removed, stored as a control, and analyzed along with the samples. The resulting solution was filtered directly on the Sterivex filter units for the air. Air was collected over c. 5 h on March 11th 2019. In addition, the two separate air DNA extractions were combined together in order to increase DNA yield. Two freshly deposited snow samples were collected on March 20th 2019 at the same site using a sterilized shovel. Both pairs of samples were separately combined in order to increase DNA yield. Snow was melted at room temperature, under strictly sterile conditions, for 24 h in the laboratory at Juan Carlos I Station and then filtered using Sterivex filters.

Etendue de l'étude Air and snow samples were collected at Punta Polaca (62°40′16″ S; 60°22′43″ W), Hurd Peninsula, Livingston Island, South Shetland Islands, near to the Spanish station Juan Carlos I in March 2019.
Contrôle qualité The control represented a field blank to certify that the samples were not contaminated by external organisms.

Description des étapes de la méthode:

  1. Total DNA was extracted from environmental samples using the Qiagen Power Soil Kit (Qiagen, USA) following the manufacturer’s instructions. Extracted DNA was used as template for generating PCR amplicons. The internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA was used as a DNA barcode for molecular species identification. PCR amplicons were generated using the universal primers ITS3 and ITS4 and were sequenced by high-throughput sequencing at Macrogen Inc. (South Korea) on an Illumina MiSeq sequencer, using the MiSeq Reagent Kit v3 (600-cycle) following the manufacturer’s protocol.

Citations bibliographiques

  1. Rosa, L. H., Pinto, O. H. B., Šantl-Temkiv, T., Convey, P., Carvalho-Silva, M., Rosa, C. A., & Câmara, P. E. (2020). DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica. Scientific reports, 10(1), 1-11.

Métadonnées additionnelles

Objet Raw DNA sequence data
Description de la fréquence de mise à jour BioProject PRJNA669512
Identifiants alternatifs https://ipt.biodiversity.aq/resource?r=fungi_snow_and_air_livingston_island