ITS samples from the air and snow of Livingston Island
Latest version published by SCAR - Microbial Antarctic Resource System on 07 June 2021 SCAR - Microbial Antarctic Resource System

Snow (n=2) and air (n=1) samples from Livingston Island (Antarctica) were analyzed for the presence of Fungi using amplicon sequencing techniques (Illumina MiSeq, ITS2 marker gene).

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Researchers should cite this work as follows:

Rosa L H, Pinto O H B, Santl-Temkiv T, Convey P, Carvalho-Silva M, Rosa C A, Camara P (2021): ITS samples from the air and snow of Livingston Island. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=fungi_snow_and_air_livingston_island&v=1.0

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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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This resource has been registered with GBIF, and assigned the following GBIF UUID: 1710f7e0-3e36-4243-be38-9f11f18f30ea.  SCAR - Microbial Antarctic Resource System publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Scientific Committee on Antarctic Research.

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Metadata

Contacts

Who created the resource:

Luis Hendrique Rosa
Universidade Federal de Minas Gerais
BR
Otavo Henrique Bezzera Pinto
BR
Tina Santl-Temkiv
DK
Peter Convey
GB
Micheline Carvalho-Silva
BR
Carlos Augusto Rosa
BR
Paulo Camara
BR

Who can answer questions about the resource:

Luiz Hendrique Rosa
Universidade Federal de Minas Gerais
BR

Who filled in the metadata:

Maxime Sweetlove
Royal Belgian Institute of Natural Sciences
Brussels
BE
Geographic Coverage

Antarctica: Livingston Island, near Spanish Juan Carlos I station

Bounding Coordinates South West [-62.671, -60.379], North East [-62.671, -60.379]
Temporal Coverage
Start Date / End Date 2019-03-11 / 2019-03-20
Project Data

No Description available

Title ITS samples from the air and snow of Livingston Island
Funding This study received financial support from CNPq, PROANTAR, FAPEMIG, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES), and INCT Criosfera 2. NERC core funding to the British Antarctic Survey’s ‘Biodiversity, Evolution and Adaptation’ Team also provided support, as well as the Spanish Polar Committee and its staff at Gabriel de Castilla base.

The personnel involved in the project:

Luiz Hendrique Rosa
Sampling Methods

Two air samples were collected with a high flow glass impinger following Šantl-Temkiv et al. The chamber was filled with 2 L of sampling liquid (ddH2O) and the sampler was run for 5 min, so that the liquid came in contact with the entire chamber, after which 0.5 L of the sampling liquid was removed, stored as a control, and analyzed along with the samples. The resulting solution was filtered directly on the Sterivex filter units for the air. Air was collected over c. 5 h on March 11th 2019. In addition, the two separate air DNA extractions were combined together in order to increase DNA yield. Two freshly deposited snow samples were collected on March 20th 2019 at the same site using a sterilized shovel. Both pairs of samples were separately combined in order to increase DNA yield. Snow was melted at room temperature, under strictly sterile conditions, for 24 h in the laboratory at Juan Carlos I Station and then filtered using Sterivex filters.

Study Extent Air and snow samples were collected at Punta Polaca (62°40′16″ S; 60°22′43″ W), Hurd Peninsula, Livingston Island, South Shetland Islands, near to the Spanish station Juan Carlos I in March 2019.
Quality Control The control represented a field blank to certify that the samples were not contaminated by external organisms.

Method step description:

  1. Total DNA was extracted from environmental samples using the Qiagen Power Soil Kit (Qiagen, USA) following the manufacturer’s instructions. Extracted DNA was used as template for generating PCR amplicons. The internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA was used as a DNA barcode for molecular species identification. PCR amplicons were generated using the universal primers ITS3 and ITS4 and were sequenced by high-throughput sequencing at Macrogen Inc. (South Korea) on an Illumina MiSeq sequencer, using the MiSeq Reagent Kit v3 (600-cycle) following the manufacturer’s protocol.
Bibliographic Citations
  1. Rosa, L. H., Pinto, O. H. B., Šantl-Temkiv, T., Convey, P., Carvalho-Silva, M., Rosa, C. A., & Câmara, P. E. (2020). DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica. Scientific reports, 10(1), 1-11.
Additional Metadata
Purpose Raw DNA sequence data
Maintenance Description BioProject PRJNA669512
Alternative Identifiers https://ipt.biodiversity.aq/resource?r=fungi_snow_and_air_livingston_island