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ITS samples from the air and snow of Livingston Island

Последняя версия опубликована SCAR - Microbial Antarctic Resource System 7 июня 2021 г. SCAR - Microbial Antarctic Resource System
Snow (n=2) and air (n=1) samples from Livingston Island (Antarctica) were analyzed for the presence of Fungi using amplicon sequencing techniques (Illumina MiSeq, ITS2 marker gene).
Дата публикации:
7 июня 2021 г.
Техническое обеспечение:
SCAR - Microbial Antarctic Resource System
Лицензия:
CC-BY 4.0

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Версии

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Как оформить ссылку

Исследователи должны дать ссылку на эту работу следующим образом:

Rosa L H, Pinto O H B, Santl-Temkiv T, Convey P, Carvalho-Silva M, Rosa C A, Camara P (2021): ITS samples from the air and snow of Livingston Island. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=fungi_snow_and_air_livingston_island&v=1.0

Права

Исследователи должны соблюдать следующие права:

Публикующей организацией и владельцем прав на данную работу является SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

Регистрация в GBIF

Этот ресурс был зарегистрирован в GBIF, ему был присвоен следующий UUID: 1710f7e0-3e36-4243-be38-9f11f18f30ea.  SCAR - Microbial Antarctic Resource System отвечает за публикацию этого ресурса, и зарегистрирован в GBIF как издатель данных при оподдержке Scientific Committee on Antarctic Research.

Ключевые слова

Metadata

Контакты

Кто является создателем ресурса:

Luis Hendrique Rosa
Universidade Federal de Minas Gerais
BR
Otavo Henrique Bezzera Pinto
BR
Tina Santl-Temkiv
DK
Peter Convey
GB
Micheline Carvalho-Silva
BR
Carlos Augusto Rosa
BR
Paulo Camara
BR

Кто может ответить на вопросы о ресурсе:

Luiz Hendrique Rosa
Universidade Federal de Minas Gerais
BR

Кем заполнены метаданные:

Maxime Sweetlove
Royal Belgian Institute of Natural Sciences
Brussels
BE

Географический охват

Antarctica: Livingston Island, near Spanish Juan Carlos I station

Ограничивающие координаты Юг Запад [-62,671, -60,379], Север Восток [-62,671, -60,379]

Временной охват

Дата начала / Дата окончания 2019-03-11 / 2019-03-20

Данные проекта

Описание отсутсвует

Название ITS samples from the air and snow of Livingston Island
Финансирование This study received financial support from CNPq, PROANTAR, FAPEMIG, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES), and INCT Criosfera 2. NERC core funding to the British Antarctic Survey’s ‘Biodiversity, Evolution and Adaptation’ Team also provided support, as well as the Spanish Polar Committee and its staff at Gabriel de Castilla base.

Исполнители проекта:

Luiz Hendrique Rosa

Методы сбора

Two air samples were collected with a high flow glass impinger following Šantl-Temkiv et al. The chamber was filled with 2 L of sampling liquid (ddH2O) and the sampler was run for 5 min, so that the liquid came in contact with the entire chamber, after which 0.5 L of the sampling liquid was removed, stored as a control, and analyzed along with the samples. The resulting solution was filtered directly on the Sterivex filter units for the air. Air was collected over c. 5 h on March 11th 2019. In addition, the two separate air DNA extractions were combined together in order to increase DNA yield. Two freshly deposited snow samples were collected on March 20th 2019 at the same site using a sterilized shovel. Both pairs of samples were separately combined in order to increase DNA yield. Snow was melted at room temperature, under strictly sterile conditions, for 24 h in the laboratory at Juan Carlos I Station and then filtered using Sterivex filters.

Охват исследования Air and snow samples were collected at Punta Polaca (62°40′16″ S; 60°22′43″ W), Hurd Peninsula, Livingston Island, South Shetland Islands, near to the Spanish station Juan Carlos I in March 2019.
Контроль качества The control represented a field blank to certify that the samples were not contaminated by external organisms.

Описание этапа методики:

  1. Total DNA was extracted from environmental samples using the Qiagen Power Soil Kit (Qiagen, USA) following the manufacturer’s instructions. Extracted DNA was used as template for generating PCR amplicons. The internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA was used as a DNA barcode for molecular species identification. PCR amplicons were generated using the universal primers ITS3 and ITS4 and were sequenced by high-throughput sequencing at Macrogen Inc. (South Korea) on an Illumina MiSeq sequencer, using the MiSeq Reagent Kit v3 (600-cycle) following the manufacturer’s protocol.

Библиографические ссылки

  1. Rosa, L. H., Pinto, O. H. B., Šantl-Temkiv, T., Convey, P., Carvalho-Silva, M., Rosa, C. A., & Câmara, P. E. (2020). DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica. Scientific reports, 10(1), 1-11.

Дополнительные метаданные

Цель Raw DNA sequence data
Описание частоты обновления ресурса BioProject PRJNA669512
Альтернативные идентификаторы https://ipt.biodiversity.aq/resource?r=fungi_snow_and_air_livingston_island