説明
Amplicon sequencing dataset (454 pyrosequencing) of Archaea (16S), Bacteria (16S) and Eukaryote (18S) in the water column of lake Fryxell and Lake Bonney, in the McMurdo Dry valleys (Antarctica).
バージョン
次の表は、公にアクセス可能な公開バージョンのリソースのみ表示しています。
引用方法
研究者はこの研究内容を以下のように引用する必要があります。:
Vick-Majors T, Priscu J, Amaral-Zettler L (2019): Microbiome (Archaea, Bacteria and Eukaryota) of lake Fryxell and lake Bonney (McMurdo Dry Valleys, Antarctica). v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbiome_mc_murdo_dry_valley_lakes_antarctica&v=1.2
権利
研究者は権利に関する下記ステートメントを尊重する必要があります。:
パブリッシャーとライセンス保持者権利者は SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF登録
このリソースをはGBIF と登録されており GBIF UUID: 4b44031f-2271-48a8-b3d9-43b90768e071が割り当てられています。 Scientific Committee on Antarctic Research によって承認されたデータ パブリッシャーとして GBIF に登録されているSCAR - Microbial Antarctic Resource System が、このリソースをパブリッシュしました。
キーワード
Metadata
連絡先
- 最初のデータ採集者 ●
- 連絡先
- 最初のデータ採集者
- 最初のデータ採集者
- メタデータ提供者
- Research assistent
- Rue Vautier 29
地理的範囲
Lake Fryxell and lake Bonney, McMurdo Dry Valleys, Antarctica
| 座標（緯度経度） | 南 西 [-77.72, 162.297], 北 東 [-77.72, 163.146] |
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生物分類学的範囲
Archaea were profiled by targeting the v6 region of the 16S ssu rRNA gene
| Domain | Archaea (Archaea) |
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Bacteria were profiled by targeting the v6 region of the 16S ssu rRNA gene
| Domain | Bacteria (Bacteria) |
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Eukaryota were profiled by targeting the v9 region of the 18S ssu rRNA gene
| Domain | Eukaryota (Eukaryotes) |
|---|
時間的範囲
| 開始日 / 終了日 | 2007-11-22 / 2008-03-25 |
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プロジェクトデータ
説明がありません
| タイトル | MIRADA-LTERS |
|---|---|
| ファンデイング | Funding was provided by NSF DEB-0717390 (MIRADA-LTERS) and OPP-1115254, OPP-0838953, OPP-1027284 and OPP- 0839075. The Montana Space Grant Consortium provided additional funding. |
プロジェクトに携わる要員:
収集方法
Duplicate samples were collected at depths of 6 m (bacterial and primary production maximum) and 9 m (chemocline; chlorophyll-a maximum) in lake Fryxell (2 November 2007 and 25 March 2008) and 13 m (chemocline; bacterial production, chlorophyll-a, primary production maximum) and 18 m (hypersaline, bottom of trophogenic zone) in lake Bonney (west Lobe; 30 November 2007 and 12 March 2008). Samples for DNA extraction were filtered onto 0.2-μM Sterivex filters (Millipore, Billerica, MA, USA) and stored with 2.0 ml of Puregene lysis buffer at −20 °C until further processing.
| Study Extent | Sampling occurred during the Austral summer and autumn in lake Fryxell and lake Bonney, Antarctica |
|---|
Method step description:
- DNA extraction protocols can be found at http://amarallab.mbl.edu.
- The V6 hypervariable regions of Archaa and bacteria were amplified, while for Eukaryota, amplification of the V9 hypervariable region followed established protocols (Amaral-Zettler et al., 2009). We multiplex-sequenced the resulting amplicons using bar-coded primers (Huber et al., 2007; Amaral-Zettler et al., 2009) on a 454 Genome Sequencer FLX (Roche, Switzerland) using the manufacturer’s recommended protocol.
書誌情報の引用
- Vick-Majors, T. J., Priscu, J. C., & Amaral-Zettler, L. A. (2014). Modular community structure suggests metabolic plasticity during the transition to polar night in ice-covered Antarctic lakes. The ISME journal, 8(4), 778.
- Xu, Y., Vick-Majors, T., Morgan-Kiss, R., Priscu, J. C., & Amaral-Zettler, L. (2014). Ciliate diversity, community structure, and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica. The Biological Bulletin, 227(2), 175-190.
追加のメタデータ
| 代替識別子 | 4b44031f-2271-48a8-b3d9-43b90768e071 |
|---|---|
| https://ipt.biodiversity.aq/resource?r=microbiome_mc_murdo_dry_valley_lakes_antarctica |