說明
Amplicon sequencing dataset (454 pyrosequencing) of Archaea (16S), Bacteria (16S) and Eukaryote (18S) in the water column of lake Fryxell and Lake Bonney, in the McMurdo Dry valleys (Antarctica).
版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Vick-Majors T, Priscu J, Amaral-Zettler L (2019): Microbiome (Archaea, Bacteria and Eukaryota) of lake Fryxell and lake Bonney (McMurdo Dry Valleys, Antarctica). v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbiome_mc_murdo_dry_valley_lakes_antarctica&v=1.2
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF 註冊
此資源已向GBIF註冊,並指定以下之GBIF UUID: 4b44031f-2271-48a8-b3d9-43b90768e071。 SCAR - Microbial Antarctic Resource System 發佈此資源,並經由Scientific Committee on Antarctic Research同意向GBIF註冊成為資料發佈者。
關鍵字
Metadata
聯絡資訊
- 出處 ●
- 連絡人
- 出處
- 出處
- 元數據提供者
- Research assistent
- Rue Vautier 29
地理涵蓋範圍
Lake Fryxell and lake Bonney, McMurdo Dry Valleys, Antarctica
界定座標範圍 | 緯度南界 經度西界 [-77.72, 162.297], 緯度北界 經度東界 [-77.72, 163.146] |
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分類群涵蓋範圍
Archaea were profiled by targeting the v6 region of the 16S ssu rRNA gene
Domain | Archaea (Archaea) |
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Bacteria were profiled by targeting the v6 region of the 16S ssu rRNA gene
Domain | Bacteria (Bacteria) |
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Eukaryota were profiled by targeting the v9 region of the 18S ssu rRNA gene
Domain | Eukaryota (Eukaryotes) |
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時間涵蓋範圍
起始日期 / 結束日期 | 2007-11-22 / 2008-03-25 |
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計畫資料
無相關描述
計畫名稱 | MIRADA-LTERS |
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經費來源 | Funding was provided by NSF DEB-0717390 (MIRADA-LTERS) and OPP-1115254, OPP-0838953, OPP-1027284 and OPP- 0839075. The Montana Space Grant Consortium provided additional funding. |
參與計畫的人員:
取樣方法
Duplicate samples were collected at depths of 6 m (bacterial and primary production maximum) and 9 m (chemocline; chlorophyll-a maximum) in lake Fryxell (2 November 2007 and 25 March 2008) and 13 m (chemocline; bacterial production, chlorophyll-a, primary production maximum) and 18 m (hypersaline, bottom of trophogenic zone) in lake Bonney (west Lobe; 30 November 2007 and 12 March 2008). Samples for DNA extraction were filtered onto 0.2-μM Sterivex filters (Millipore, Billerica, MA, USA) and stored with 2.0 ml of Puregene lysis buffer at −20 °C until further processing.
研究範圍 | Sampling occurred during the Austral summer and autumn in lake Fryxell and lake Bonney, Antarctica |
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方法步驟描述:
- DNA extraction protocols can be found at http://amarallab.mbl.edu.
- The V6 hypervariable regions of Archaa and bacteria were amplified, while for Eukaryota, amplification of the V9 hypervariable region followed established protocols (Amaral-Zettler et al., 2009). We multiplex-sequenced the resulting amplicons using bar-coded primers (Huber et al., 2007; Amaral-Zettler et al., 2009) on a 454 Genome Sequencer FLX (Roche, Switzerland) using the manufacturer’s recommended protocol.
引用文獻
- Vick-Majors, T. J., Priscu, J. C., & Amaral-Zettler, L. A. (2014). Modular community structure suggests metabolic plasticity during the transition to polar night in ice-covered Antarctic lakes. The ISME journal, 8(4), 778.
- Xu, Y., Vick-Majors, T., Morgan-Kiss, R., Priscu, J. C., & Amaral-Zettler, L. (2014). Ciliate diversity, community structure, and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica. The Biological Bulletin, 227(2), 175-190.
額外的詮釋資料
替代的識別碼 | 4b44031f-2271-48a8-b3d9-43b90768e071 |
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https://ipt.biodiversity.aq/resource?r=microbiome_mc_murdo_dry_valley_lakes_antarctica |