Description
Amplicon sequencing dataset targeting eukaryote microorganisms (18S ssu rRNA marker gene) in permanently ice-covered lakes in the McMurdo Dry Valleys (Antarctica). Samples (n=89) were taken in the photic zone of the east lobe (ELB) and west lobe (WLB) of the chemically stratified Lake Bonney.
Versions
Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.
Comment citer
Les chercheurs doivent citer cette ressource comme suit:
Bielewicz S, Bell E, Kong W, Froedberg I, Priscu J, Morgan-Kiss R (2021): Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=protist_diversity_in_antarctic_lake_during_polar_night&v=1.1
Droits
Les chercheurs doivent respecter la déclaration de droits suivante:
L’éditeur et détenteur des droits de cette ressource est SCAR - Microbial Antarctic Resource System. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.
Enregistrement GBIF
Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 8d83b6ed-c88d-4bf6-90a2-c6ade46fee16. SCAR - Microbial Antarctic Resource System publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du Scientific Committee on Antarctic Research.
Mots-clé
Metadata
Contacts
- Créateur
- Créateur
- Créateur
- Créateur
- Créateur
- Créateur ●
- Utilisateur ●
- Personne De Contact
- Fournisseur Des Métadonnées
Couverture géographique
Lake Bonney, McMurdo Dry Valleys, Antarctica West = East =
| Enveloppe géographique | Sud Ouest [-77,435, 161,405], Nord Est [-77,316, 163,743] |
|---|
Couverture temporelle
| Date de début / Date de fin | 2010-11-18 / 2011-03-15 |
|---|
Données sur le projet
Mc Murdo Dry Valleys Long Term Ecological Research Project (LTER; http://www.mcmlter.org/)
| Titre | Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition |
|---|---|
| Financement | National Science Foundation (NSF) |
Les personnes impliquées dans le projet:
Méthodes d'échantillonnage
Samples (1 liter) were vacuum concentrated (5 kPa) onto 47 mm 0.45 µm Durapore polyvinylidene fluoride membrane filters (Millipore). The filters were frozen immediately in liquid nitrogen and transported frozen to McMurdo Station, where they were stored at -80 degrees C until DNA extraction.
| Etendue de l'étude | Lake Bonney in the McMurdo Dry Valleys (Antarctica). Samples were collected at 6, 13, 18, and 20m in the east lobe (ELB) and 10, 13, 15, and 20m in the west lobe (WLB) of Lake Bonney from February to April 2008. All collection depths are relative to the piezometric water level in the ice borehole. |
|---|
Description des étapes de la méthode:
- DNA was isolated from a whole filter using the MP DNA kit (MP Biomedicals, CA) following the kit’s instruction. Sequencing was performed using the BigDye Terminator v3.1 cycle sequencing kit (ABI, CA) with M13R primer and the fragments were sequenced on an Applied Biosystems 3730×l DNA Analyzer (ABI, CA). The 18S rRNA gene sequences reported in the current study have been deposited in GenBank under accession numbers GU969060 to GU969102.
Citations bibliographiques
- Bielewicz, S., Bell, E., Kong, W., Friedberg, I., Priscu, J. C., & Morgan-Kiss, R. M. (2011). Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition. The ISME Journal, 5(9), 1559–1564. doi:10.1038/ismej.2011.23
Métadonnées additionnelles
| Identifiants alternatifs | https://ipt.biodiversity.aq/resource?r=protist_diversity_in_antarctic_lake_during_polar_night |
|---|