Descrição
Amplicon sequencing dataset targeting eukaryote microorganisms (18S ssu rRNA marker gene) in permanently ice-covered lakes in the McMurdo Dry Valleys (Antarctica). Samples (n=89) were taken in the photic zone of the east lobe (ELB) and west lobe (WLB) of the chemically stratified Lake Bonney.
Versões
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Como citar
Pesquisadores deveriam citar esta obra da seguinte maneira:
Bielewicz S, Bell E, Kong W, Froedberg I, Priscu J, Morgan-Kiss R (2021): Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=protist_diversity_in_antarctic_lake_during_polar_night&v=1.1
Direitos
Pesquisadores devem respeitar a seguinte declaração de direitos:
O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF Registration
Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 8d83b6ed-c88d-4bf6-90a2-c6ade46fee16. SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.
Palavras-chave
Metadata
Contatos
- Originador
- Originador
- Originador
- Originador
- Originador
- Originador ●
- Usuário ●
- Ponto De Contato
- Provedor Dos Metadados
Cobertura Geográfica
Lake Bonney, McMurdo Dry Valleys, Antarctica West = East =
| Coordenadas delimitadoras | Sul Oeste [-77,435, 161,405], Norte Leste [-77,316, 163,743] |
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Cobertura Temporal
| Data Inicial / Data final | 2010-11-18 / 2011-03-15 |
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Dados Sobre o Projeto
Mc Murdo Dry Valleys Long Term Ecological Research Project (LTER; http://www.mcmlter.org/)
| Título | Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition |
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| Financiamento | National Science Foundation (NSF) |
O pessoal envolvido no projeto:
Métodos de Amostragem
Samples (1 liter) were vacuum concentrated (5 kPa) onto 47 mm 0.45 µm Durapore polyvinylidene fluoride membrane filters (Millipore). The filters were frozen immediately in liquid nitrogen and transported frozen to McMurdo Station, where they were stored at -80 degrees C until DNA extraction.
| Área de Estudo | Lake Bonney in the McMurdo Dry Valleys (Antarctica). Samples were collected at 6, 13, 18, and 20m in the east lobe (ELB) and 10, 13, 15, and 20m in the west lobe (WLB) of Lake Bonney from February to April 2008. All collection depths are relative to the piezometric water level in the ice borehole. |
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Descrição dos passos do método:
- DNA was isolated from a whole filter using the MP DNA kit (MP Biomedicals, CA) following the kit’s instruction. Sequencing was performed using the BigDye Terminator v3.1 cycle sequencing kit (ABI, CA) with M13R primer and the fragments were sequenced on an Applied Biosystems 3730×l DNA Analyzer (ABI, CA). The 18S rRNA gene sequences reported in the current study have been deposited in GenBank under accession numbers GU969060 to GU969102.
Citações bibliográficas
- Bielewicz, S., Bell, E., Kong, W., Friedberg, I., Priscu, J. C., & Morgan-Kiss, R. M. (2011). Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition. The ISME Journal, 5(9), 1559–1564. doi:10.1038/ismej.2011.23
Metadados Adicionais
| Identificadores alternativos | https://ipt.biodiversity.aq/resource?r=protist_diversity_in_antarctic_lake_during_polar_night |
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