Aeolian microbial dispersal limitation to Antarctica (2017)

元數據
最新版本 published by SCAR - Microbial Antarctic Resource System on 六月 16, 2021 SCAR - Microbial Antarctic Resource System
發布日期:
2021年6月16日
授權條款:
CC-BY 4.0

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說明

Amplicon sequencing dataset (Illumina MiSeq) targeting Bacteria (16S ssu rRNA) and Fungi (ITS) in samples (n=110) from soils and aerosol in the McMurdo Dry Valleys and New Zealand.

版本

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如何引用

研究者應依照以下指示引用此資源。:

Archer S, Maestre F, Maki T, Lee C, Cowan D, Pointing S (2021): Aeolian microbial dispersal limitation to Antarctica (2017). v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=aeolian_microbes_antarctica&v=1.0

權利

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此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF 註冊

此資源已向GBIF註冊，並指定以下之GBIF UUID: 800104b4-cea6-41d7-8944-0fdcca7a7aa8。  SCAR - Microbial Antarctic Resource System 發佈此資源，並經由Scientific Committee on Antarctic Research同意向GBIF註冊成為資料發佈者。

關鍵字

Metadata

聯絡資訊

Stephen Archer
  • 出處 ●
  • 使用者 ●
  • 連絡人
Institute for Applied Ecology New Zealand
Auckland
NZ
Fernando Maestre
  • 出處
Universidad Rey Juan Carlos
Mostoles
ES
Teruya Maki
  • 出處
Kanazawa University
Kanazawa
JP
Charles Lee
  • 出處
University of Waikato
Hamilton
NZ
Don Cowan
  • 出處
University of Pretoria
Pretoria
ZA
Stephen Pointing
  • 出處
Institute for Applied Ecology New Zealand
Auckland
NZ
Maxime Sweetlove
  • 元數據提供者
Royal Belgian Institute of Natural Sciences
Brussels
BE

地理涵蓋範圍

Antarctica:McMurdo Dry Valleys:Bull Pass, Valley ridge; New Zealand:Glen Eden, Auckland

界定座標範圍 緯度南界 經度西界 [-77.519, 161.769], 緯度北界 經度東界 [-36.916, 174.646]

計畫資料

無相關描述

計畫名稱 Aeolian microbial dispersal limitation to Antarctica (2017)
經費來源 Funding was provided by a grant from the New Zealand Ministry of Business, Innovation & Employment (UOWX1401) and the Yale-NUS College Start-Up Fund. Additional support was given by the European Research Council (BIODESERT project, ERC Grant agreement n° 647038).

參與計畫的人員:

Stephen Archer

取樣方法

Sampling of air mass above the boundary layer for surface influence was achieved by mounting the apparatus in a helicopter with an external sampling port.

研究範圍 Air mass at near-surface (1.5m above ground) and underlying soil was sampled from 11 to 23 January 2017 at valley and elevated locations throughout the Wright Valley, McMurdo Dry Valleys, Antarctica. Comparison samples were taken from the North Island of New Zealand.

方法步驟描述:

  1. Total DNA was directly extracted using a CTAB protocol. DNA yield for these ultra-low biomass samples was quantified using the Qubit 2.0 Fluorometer (Invitrogen) in the range 1.06-8.44ng. Samples were then stored at -20 C. Illumina MiSeq libraries were prepared as per manufacturer’s protocol (Metagenomic Sequencing Library Preparation Part # 15044223 Rev. B; Illumina, San Diego, CA, USA) and with PhiX positive controls. Bacteria and Fungi were targeted with domain-specific PCR primers PCR1 forward (5’ TCGTCGGCAGCGTCAGATGT GTATAAGAGA CAGCCTACGG GNGGCWGCAG 3’) and PCR1 reverse (5’ GTCTCGTGGG CTCGGAGATG TGTATAAGAG ACAGGACTAC HVGGGTATCT AATCC 3’) and the internal transcribed spacer region of fungal 18S and 5.8S rRNA genes: ITS1 forward (5' CTTGGTCATTTAGAGGAAGTAA 3') and ITS2 reverse (5' GCTGCGTTCTTCATCGATGC 3').

引用文獻

  1. Archer, S., Lee, K., Caruso, T., Maki, T., Lee, C., Cowan, D., ... & Pointing, S. (2018). Microbial dispersal limitation to isolated soil habitats in the McMurdo Dry Valleys of Antarctica. bioRxiv, 493411. https://doi.org/10.1101/493411