Aeolian microbial dispersal limitation to Antarctica (2017)

Latest version published by SCAR - Microbial Antarctic Resource System on Jun 16, 2021 SCAR - Microbial Antarctic Resource System
Publication date:
16 June 2021
CC-BY 4.0

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Amplicon sequencing dataset (Illumina MiSeq) targeting Bacteria (16S ssu rRNA) and Fungi (ITS) in samples (n=110) from soils and aerosol in the McMurdo Dry Valleys and New Zealand.


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Researchers should cite this work as follows:

Archer S, Maestre F, Maki T, Lee C, Cowan D, Pointing S (2021): Aeolian microbial dispersal limitation to Antarctica (2017). v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata.


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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 800104b4-cea6-41d7-8944-0fdcca7a7aa8.  SCAR - Microbial Antarctic Resource System publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Scientific Committee on Antarctic Research.




Stephen Archer
  • Originator
  • User
  • Point Of Contact
Institute for Applied Ecology New Zealand
Fernando Maestre
  • Originator
Universidad Rey Juan Carlos
Teruya Maki
  • Originator
Kanazawa University
Charles Lee
  • Originator
University of Waikato
Don Cowan
  • Originator
University of Pretoria
Stephen Pointing
  • Originator
Institute for Applied Ecology New Zealand
Maxime Sweetlove
  • Metadata Provider
Royal Belgian Institute of Natural Sciences

Geographic Coverage

Antarctica:McMurdo Dry Valleys:Bull Pass, Valley ridge; New Zealand:Glen Eden, Auckland

Bounding Coordinates South West [-77.519, 161.769], North East [-36.916, 174.646]

Project Data

No Description available

Title Aeolian microbial dispersal limitation to Antarctica (2017)
Funding Funding was provided by a grant from the New Zealand Ministry of Business, Innovation & Employment (UOWX1401) and the Yale-NUS College Start-Up Fund. Additional support was given by the European Research Council (BIODESERT project, ERC Grant agreement n° 647038).

The personnel involved in the project:

Stephen Archer

Sampling Methods

Sampling of air mass above the boundary layer for surface influence was achieved by mounting the apparatus in a helicopter with an external sampling port.

Study Extent Air mass at near-surface (1.5m above ground) and underlying soil was sampled from 11 to 23 January 2017 at valley and elevated locations throughout the Wright Valley, McMurdo Dry Valleys, Antarctica. Comparison samples were taken from the North Island of New Zealand.

Method step description:

  1. Total DNA was directly extracted using a CTAB protocol. DNA yield for these ultra-low biomass samples was quantified using the Qubit 2.0 Fluorometer (Invitrogen) in the range 1.06-8.44ng. Samples were then stored at -20 C. Illumina MiSeq libraries were prepared as per manufacturer’s protocol (Metagenomic Sequencing Library Preparation Part # 15044223 Rev. B; Illumina, San Diego, CA, USA) and with PhiX positive controls. Bacteria and Fungi were targeted with domain-specific PCR primers PCR1 forward (5’ TCGTCGGCAGCGTCAGATGT GTATAAGAGA CAGCCTACGG GNGGCWGCAG 3’) and PCR1 reverse (5’ GTCTCGTGGG CTCGGAGATG TGTATAAGAG ACAGGACTAC HVGGGTATCT AATCC 3’) and the internal transcribed spacer region of fungal 18S and 5.8S rRNA genes: ITS1 forward (5' CTTGGTCATTTAGAGGAAGTAA 3') and ITS2 reverse (5' GCTGCGTTCTTCATCGATGC 3').

Bibliographic Citations

  1. Archer, S., Lee, K., Caruso, T., Maki, T., Lee, C., Cowan, D., ... & Pointing, S. (2018). Microbial dispersal limitation to isolated soil habitats in the McMurdo Dry Valleys of Antarctica. bioRxiv, 493411.

Additional Metadata