Aeolian microbial dispersal limitation to Antarctica (2017)
Latest version published by SCAR - Microbial Antarctic Resource System on 16 June 2021 SCAR - Microbial Antarctic Resource System

Amplicon sequencing dataset (Illumina MiSeq) targeting Bacteria (16S ssu rRNA) and Fungi (ITS) in samples (n=110) from soils and aerosol in the McMurdo Dry Valleys and New Zealand.

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Researchers should cite this work as follows:

Archer S, Maestre F, Maki T, Lee C, Cowan D, Pointing S (2021): Aeolian microbial dispersal limitation to Antarctica (2017). v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=aeolian_microbes_antarctica&v=1.0

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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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This resource has been registered with GBIF, and assigned the following GBIF UUID: 800104b4-cea6-41d7-8944-0fdcca7a7aa8.  SCAR - Microbial Antarctic Resource System publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Scientific Committee on Antarctic Research.

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Contacts

Who created the resource:

Stephen Archer
Institute for Applied Ecology New Zealand
Auckland
NZ
Fernando Maestre
Universidad Rey Juan Carlos
Mostoles
ES
Teruya Maki
Kanazawa University
Kanazawa
JP
Charles Lee
University of Waikato
Hamilton
NZ
Don Cowan
University of Pretoria
Pretoria
ZA
Stephen Pointing
Institute for Applied Ecology New Zealand
Auckland
NZ

Who can answer questions about the resource:

Stephen Archer
Institute for Applied Ecology New Zealand
Auckland
NZ

Who filled in the metadata:

Maxime Sweetlove
Royal Belgian Institute of Natural Sciences
Brussels
BE

Who else was associated with the resource:

User
Stephen Archer
Institute for Applied Ecology New Zealand
Auckland
NZ
Geographic Coverage

Antarctica:McMurdo Dry Valleys:Bull Pass, Valley ridge; New Zealand:Glen Eden, Auckland

Bounding Coordinates South West [-77.519, 161.769], North East [-36.916, 174.646]
Project Data

No Description available

Title Aeolian microbial dispersal limitation to Antarctica (2017)
Funding Funding was provided by a grant from the New Zealand Ministry of Business, Innovation & Employment (UOWX1401) and the Yale-NUS College Start-Up Fund. Additional support was given by the European Research Council (BIODESERT project, ERC Grant agreement n° 647038).

The personnel involved in the project:

Stephen Archer
Sampling Methods

Sampling of air mass above the boundary layer for surface influence was achieved by mounting the apparatus in a helicopter with an external sampling port.

Study Extent Air mass at near-surface (1.5m above ground) and underlying soil was sampled from 11 to 23 January 2017 at valley and elevated locations throughout the Wright Valley, McMurdo Dry Valleys, Antarctica. Comparison samples were taken from the North Island of New Zealand.

Method step description:

  1. Total DNA was directly extracted using a CTAB protocol. DNA yield for these ultra-low biomass samples was quantified using the Qubit 2.0 Fluorometer (Invitrogen) in the range 1.06-8.44ng. Samples were then stored at -20 C. Illumina MiSeq libraries were prepared as per manufacturer’s protocol (Metagenomic Sequencing Library Preparation Part # 15044223 Rev. B; Illumina, San Diego, CA, USA) and with PhiX positive controls. Bacteria and Fungi were targeted with domain-specific PCR primers PCR1 forward (5’ TCGTCGGCAGCGTCAGATGT GTATAAGAGA CAGCCTACGG GNGGCWGCAG 3’) and PCR1 reverse (5’ GTCTCGTGGG CTCGGAGATG TGTATAAGAG ACAGGACTAC HVGGGTATCT AATCC 3’) and the internal transcribed spacer region of fungal 18S and 5.8S rRNA genes: ITS1 forward (5' CTTGGTCATTTAGAGGAAGTAA 3') and ITS2 reverse (5' GCTGCGTTCTTCATCGATGC 3').
Bibliographic Citations
  1. Archer, S., Lee, K., Caruso, T., Maki, T., Lee, C., Cowan, D., ... & Pointing, S. (2018). Microbial dispersal limitation to isolated soil habitats in the McMurdo Dry Valleys of Antarctica. bioRxiv, 493411. https://doi.org/10.1101/493411
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