Antarctic polar desert surface soil microbiome

Versão mais recente publicado por SCAR - Microbial Antarctic Resource System em Mar 28, 2019 SCAR - Microbial Antarctic Resource System

Whole-genome shotgun sequencing dataset targeting microbes in three soil samples from pristine polar desert at Robinson Ridge (eastern Antarctica)

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Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Ji M, Greening C, Vanwonterghem I, Carere C, Bay S, Steen J, Montgomery K, Lines T, van Dorst J, Snape I, Stott M, Hugenholtz P, Ferrari B (2019): Antarctic polar desert surface soil microbiome. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=antarctic_desert_surface_soil_microbiome&v=1.0

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O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: ef36ddd4-b37c-4467-9ad4-eb77d679959b.  SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.

Palavras-chave

Metadata

Contatos

Quem criou esse recurso:

Mukan Ji
Australian Centre for Astrobiology Randwick AU
Chris Greening
Monash University Clayton AU
Inka Vanwonterghem
University of Queensland St Lucia AU
Carlo Carere
Wairakei Research Centre Wairakei NZ
Sean Bay
Monash University Clayton AU
Jason Steen
University of Queensland St. Lucia AU
Kate Montgomery
Australian Centre for Astrobiology Randwick AU
Thomas Lines
Monash University Clayton AU
Josie van Dorst
Australian Centre for Astrobiology Randwick AU
Ian Snape
Australian Antarctic Division Kingston AU
Matthew Stott
Wairakei Research Centre Wairakei NZ
Philip Hugenholtz
University of Queensland St. Lucia AU
Belinda Ferrari
Australian Centre for Astrobiology Randwick AU

Quem pode responder a perguntas sobre o recurso:

Mukan Ji
Australian Centre for Astrobiology Randwick AU
Chris Greening
Monash University Clayton AU
Belinda Ferrari
Australian Centre for Astrobiology Randwick AU

Quem preencher os metadados:

Maxime Sweetlove
Research assistent
Royal Belgian Institute of Natural Sciences Rue Vautier 29 1000 Brussels BE

Quem mais foi associado com o recurso:

Usuário

Cobertura Geográfica

Robinson Ridge, east Antarctica

Coordenadas delimitadoras Sul Oeste [-66.37, 110.586], Norte Leste [-66.37, 110.586]

Cobertura Taxonômica

whole genome shotgun sequencing

Domínio  Bacteria (Bacteria),  Archaea (Archaea),  Eukaryota (Eukaryotes),  Viri (viruses)

Cobertura Temporal

Data Inicial 2015-12-13

Dados Sobre o Projeto

Nenhuma descrição disponível

Título Bioplatforms Australia
Financiamento This work was supported by Bioplatforms Australia, an Australian Antarctic Science project grant (4406), an ARC Future Fellowship (FT170100341), an ARC DECRA Fellowship (DE170100310), an ARC DORA and Laureate Fellowship (DP120103498 and FL150100038), and a Marsden Grant (16-GNS-035). UNSW Sydney, the Centre for Geometric Biology (Monash University), and the Geothermal Resources of New Zealand research program (GNS Science) also provided funding to support this research.

O pessoal envolvido no projeto:

Mukan Ji

Métodos de Amostragem

Samples were collected along a spatially explicit sampling design comprised of three 300-m-long transects, separated by 2-m distances from each other. Samples were sieved down to 63 μm, aliquoted into 5–25-g subsamples, and stored at −80 °C until analysis.

Área de Estudo samples were taken at the ice free Robinson Ridge (−66.367739, 110.585262), located 10 km south of Casey station in the Windmill Islands coast of Wilkes Land, is part of a pristine polar desert. Three surface soil samples (100 g) from the top 10 cm of the soil profile were collected in December 2005.

Descrição dos passos do método:

  1. Total community DNA was extracted from 0.25–0.3 g of each sample in technical triplicate using the FastDNA SPIN Kit for Soil (MP Biomedicals). All DNA extracts were quantified and DNA lysate quality was evaluated using automated ribosomal intergenic spacer analysis (ARISA).
  2. Metagenome libraries were prepared using the Nextera DNA Library Preparation Kit (Illumina) and sequenced using three-fifths of an Illumina HiSeq2000 flowcell lane at the Institute for Molecular Biosciences (University of Queensland).

Citações bibliográficas

  1. Ji, M., Greening, C., Vanwonterghem, I., Carere, C. R., Bay, S. K., Steen, J. A., ... & Snape, I. (2017). Atmospheric trace gases support primary production in Antarctic desert surface soil. Nature, 552(7685), 400. https://doi.org/10.1038/nature25014