Antarctic polar desert surface soil microbiome

最新版本 由 SCAR - Microbial Antarctic Resource System 發佈於 Mar 28, 2019 SCAR - Microbial Antarctic Resource System

Whole-genome shotgun sequencing dataset targeting microbes in three soil samples from pristine polar desert at Robinson Ridge (eastern Antarctica)

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Ji M, Greening C, Vanwonterghem I, Carere C, Bay S, Steen J, Montgomery K, Lines T, van Dorst J, Snape I, Stott M, Hugenholtz P, Ferrari B (2019): Antarctic polar desert surface soil microbiome. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=antarctic_desert_surface_soil_microbiome&v=1.0

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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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關鍵字

Metadata

聯絡資訊

資源建立者:

Mukan Ji
Australian Centre for Astrobiology Randwick AU
Chris Greening
Monash University Clayton AU
Inka Vanwonterghem
University of Queensland St Lucia AU
Carlo Carere
Wairakei Research Centre Wairakei NZ
Sean Bay
Monash University Clayton AU
Jason Steen
University of Queensland St. Lucia AU
Kate Montgomery
Australian Centre for Astrobiology Randwick AU
Thomas Lines
Monash University Clayton AU
Josie van Dorst
Australian Centre for Astrobiology Randwick AU
Ian Snape
Australian Antarctic Division Kingston AU
Matthew Stott
Wairakei Research Centre Wairakei NZ
Philip Hugenholtz
University of Queensland St. Lucia AU
Belinda Ferrari
Australian Centre for Astrobiology Randwick AU

可回覆此資源相關問題者:

Mukan Ji
Australian Centre for Astrobiology Randwick AU
Chris Greening
Monash University Clayton AU
Belinda Ferrari
Australian Centre for Astrobiology Randwick AU

元數據填寫者:

Maxime Sweetlove
Research assistent
Royal Belgian Institute of Natural Sciences Rue Vautier 29 1000 Brussels BE

與此資源的相關者:

使用者

地理涵蓋範圍

Robinson Ridge, east Antarctica

界定座標範圍 緯度南界 經度西界 [-66.37, 110.586], 緯度北界 經度東界 [-66.37, 110.586]

分類群涵蓋範圍

whole genome shotgun sequencing

Domain  Bacteria (Bacteria),  Archaea (Archaea),  Eukaryota (Eukaryotes),  Viri (viruses)

時間涵蓋範圍

起始日期 2015-12-13

計畫資料

無相關描述

計畫名稱 Bioplatforms Australia
經費來源 This work was supported by Bioplatforms Australia, an Australian Antarctic Science project grant (4406), an ARC Future Fellowship (FT170100341), an ARC DECRA Fellowship (DE170100310), an ARC DORA and Laureate Fellowship (DP120103498 and FL150100038), and a Marsden Grant (16-GNS-035). UNSW Sydney, the Centre for Geometric Biology (Monash University), and the Geothermal Resources of New Zealand research program (GNS Science) also provided funding to support this research.

The personnel involved in the project:

Mukan Ji

取樣方法

Samples were collected along a spatially explicit sampling design comprised of three 300-m-long transects, separated by 2-m distances from each other. Samples were sieved down to 63 μm, aliquoted into 5–25-g subsamples, and stored at −80 °C until analysis.

研究範圍 samples were taken at the ice free Robinson Ridge (−66.367739, 110.585262), located 10 km south of Casey station in the Windmill Islands coast of Wilkes Land, is part of a pristine polar desert. Three surface soil samples (100 g) from the top 10 cm of the soil profile were collected in December 2005.

方法步驟描述:

  1. Total community DNA was extracted from 0.25–0.3 g of each sample in technical triplicate using the FastDNA SPIN Kit for Soil (MP Biomedicals). All DNA extracts were quantified and DNA lysate quality was evaluated using automated ribosomal intergenic spacer analysis (ARISA).
  2. Metagenome libraries were prepared using the Nextera DNA Library Preparation Kit (Illumina) and sequenced using three-fifths of an Illumina HiSeq2000 flowcell lane at the Institute for Molecular Biosciences (University of Queensland).

引用文獻

  1. Ji, M., Greening, C., Vanwonterghem, I., Carere, C. R., Bay, S. K., Steen, J. A., ... & Snape, I. (2017). Atmospheric trace gases support primary production in Antarctic desert surface soil. Nature, 552(7685), 400. https://doi.org/10.1038/nature25014