Descripción
Cyanobacterial 16S rRNA gene sequences from cyanobacterial mats of Antarctic (Byers Peninsula) origin obtained by clone library
Versiones
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Derechos
Los usuarios deben respetar los siguientes derechos de uso:
El publicador y propietario de los derechos de este trabajo es SCAR - Microbial Antarctic Resource System. Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento (CC-BY 4.0).
Registro GBIF
Este recurso no ha sido registrado en GBIF
Palabras clave
Metadata
Contactos
- Originador ●
- Punto De Contacto
- Researcher
- Proveedor De Los Metadatos
- Punto De Contacto
- Researcher
Cobertura geográfica
Byers Peninsula, Livingston Island, Antarctica
Coordenadas límite | Latitud Mínima Longitud Mínima [-62,7, -61,5], Latitud Máxima Longitud Máxima [-62,5, -61] |
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Cobertura taxonómica
All data provided are OTUs of the 16S rRNA gene which were identified via a comparison to online databases to family or genus level. For most OTUs identification to species level was not possible
Filo | Cyanobacteria (Cyanobacteria) |
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Cobertura temporal
Fecha Inicial / Fecha Final | 2009-02-01 / 2009-02-28 |
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Datos del proyecto
No hay descripción disponible
Título | DI698/18-1 Dietrich |
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Fuentes de Financiación | Deutsche Forschungsgesellschaft DFG |
Personas asociadas al proyecto:
- Investigador Principal
Métodos de muestreo
Cyanobacterial samples were collected from five different sampling sites in the Antarctic Specially Protected Area (ASPA) No. 126 of Byers Peninsula, Livingston Island (62° 34' 35" to 62° 4' 35" S and 60° 54' 14" to 61° 13' 07" W), South Shetland Islands, Antarctic Peninsula, during an expedition in February 2009. Microbial mats were probed using a sterile spatula, sealed in sterile plastic bags or tubes and stored frozen (-20 °C) for DNA extraction.
Área de Estudio | Samples were collected on Byers Peninsula (ASPA 126) in February 2009. This area is a summer snow- and ice-free area. Various microbial mats on wet soil, meltwater and seepages were sampled. |
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Control de Calidad | Data were published in a peer-reviewed journal DOI: 10.1038/NCLIMATE1418 |
Descripción de la metodología paso a paso:
- After collection the samples were stored at -20°C until further processing. DNA was extracted from the samples in three replicates and combined. 16S rRNA genes were amplified using cyanobacteria specific primers (Saker et al. 2005). PCR Products were cloned using the TOPO TA Cloning Kit following the standard protocol. Two to three clones of each individual restriction fragment length polymorphism pattern were sequenced at GATC Biotech. All sequences were deposited with the GenBank database. Saker, M. L., Jungblut, A. D., Neilan, B. A., Rawn, D. F. K. & Vasconcelos, V. M. Detection of microcystin synthetase genes in health food supplements containing the freshwater cyanobacterium Aphanizomenon flos-aquae. Toxicon 46, 555?562 (2005).
Metadatos adicionales
Identificadores alternativos | https://ipt.biodiversity.aq/resource?r=cyano_16s |
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