Описание
Cyanobacterial 16S rRNA gene sequences from cyanobacterial mats of Antarctic (Byers Peninsula) origin obtained by clone library
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Публикующей организацией и владельцем прав на данную работу является SCAR - Microbial Antarctic Resource System. Эта работа находится под лицензией Creative Commons Attribution (CC-BY 4.0).
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Географический охват
Byers Peninsula, Livingston Island, Antarctica
| Ограничивающие координаты | Юг Запад [-62,7, -61,5], Север Восток [-62,5, -61] |
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Таксономический охват
All data provided are OTUs of the 16S rRNA gene which were identified via a comparison to online databases to family or genus level. For most OTUs identification to species level was not possible
| Phylum | Cyanobacteria (Cyanobacteria) |
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Временной охват
| Дата начала / Дата окончания | 2009-02-01 / 2009-02-28 |
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Данные проекта
Описание отсутсвует
| Название | DI698/18-1 Dietrich |
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| Финансирование | Deutsche Forschungsgesellschaft DFG |
Исполнители проекта:
- Principal Investigator
Методы сбора
Cyanobacterial samples were collected from five different sampling sites in the Antarctic Specially Protected Area (ASPA) No. 126 of Byers Peninsula, Livingston Island (62° 34' 35" to 62° 4' 35" S and 60° 54' 14" to 61° 13' 07" W), South Shetland Islands, Antarctic Peninsula, during an expedition in February 2009. Microbial mats were probed using a sterile spatula, sealed in sterile plastic bags or tubes and stored frozen (-20 °C) for DNA extraction.
| Охват исследования | Samples were collected on Byers Peninsula (ASPA 126) in February 2009. This area is a summer snow- and ice-free area. Various microbial mats on wet soil, meltwater and seepages were sampled. |
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| Контроль качества | Data were published in a peer-reviewed journal DOI: 10.1038/NCLIMATE1418 |
Описание этапа методики:
- After collection the samples were stored at -20°C until further processing. DNA was extracted from the samples in three replicates and combined. 16S rRNA genes were amplified using cyanobacteria specific primers (Saker et al. 2005). PCR Products were cloned using the TOPO TA Cloning Kit following the standard protocol. Two to three clones of each individual restriction fragment length polymorphism pattern were sequenced at GATC Biotech. All sequences were deposited with the GenBank database. Saker, M. L., Jungblut, A. D., Neilan, B. A., Rawn, D. F. K. & Vasconcelos, V. M. Detection of microcystin synthetase genes in health food supplements containing the freshwater cyanobacterium Aphanizomenon flos-aquae. Toxicon 46, 555?562 (2005).
Дополнительные метаданные
| Альтернативные идентификаторы | https://ipt.biodiversity.aq/resource?r=cyano_16s |
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