說明
Cyanobacterial 16S rRNA gene sequences from cyanobacterial mats of Antarctic (Byers Peninsula) origin obtained by clone library
版本
以下的表格只顯示可公開存取資源的已發布版本。
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF 註冊
此資源尚未向GBIF註冊
關鍵字
Metadata
聯絡資訊
- 元數據提供者
- 連絡人
- Researcher
地理涵蓋範圍
Byers Peninsula, Livingston Island, Antarctica
| 界定座標範圍 | 緯度南界 經度西界 [-62.7, -61.5], 緯度北界 經度東界 [-62.5, -61] |
|---|
分類群涵蓋範圍
All data provided are OTUs of the 16S rRNA gene which were identified via a comparison to online databases to family or genus level. For most OTUs identification to species level was not possible
| Phylum | Cyanobacteria (Cyanobacteria) |
|---|
時間涵蓋範圍
| 起始日期 / 結束日期 | 2009-02-01 / 2009-02-28 |
|---|
計畫資料
無相關描述
| 計畫名稱 | DI698/18-1 Dietrich |
|---|---|
| 經費來源 | Deutsche Forschungsgesellschaft DFG |
參與計畫的人員:
- 研究主持人
取樣方法
Cyanobacterial samples were collected from five different sampling sites in the Antarctic Specially Protected Area (ASPA) No. 126 of Byers Peninsula, Livingston Island (62° 34' 35" to 62° 4' 35" S and 60° 54' 14" to 61° 13' 07" W), South Shetland Islands, Antarctic Peninsula, during an expedition in February 2009. Microbial mats were probed using a sterile spatula, sealed in sterile plastic bags or tubes and stored frozen (-20 °C) for DNA extraction.
| 研究範圍 | Samples were collected on Byers Peninsula (ASPA 126) in February 2009. This area is a summer snow- and ice-free area. Various microbial mats on wet soil, meltwater and seepages were sampled. |
|---|---|
| 品質控管 | Data were published in a peer-reviewed journal DOI: 10.1038/NCLIMATE1418 |
方法步驟描述:
- After collection the samples were stored at -20°C until further processing. DNA was extracted from the samples in three replicates and combined. 16S rRNA genes were amplified using cyanobacteria specific primers (Saker et al. 2005). PCR Products were cloned using the TOPO TA Cloning Kit following the standard protocol. Two to three clones of each individual restriction fragment length polymorphism pattern were sequenced at GATC Biotech. All sequences were deposited with the GenBank database. Saker, M. L., Jungblut, A. D., Neilan, B. A., Rawn, D. F. K. & Vasconcelos, V. M. Detection of microcystin synthetase genes in health food supplements containing the freshwater cyanobacterium Aphanizomenon flos-aquae. Toxicon 46, 555?562 (2005).