cyanobacterial mats of from the Byers Peninsula, Antarctica

Dernière version Publié par SCAR - Microbial Antarctic Resource System le mars 19, 2019 SCAR - Microbial Antarctic Resource System
Date de publication:
19 mars 2019
Licence:
CC-BY 4.0

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Description

Cyanobacterial 16S rRNA gene sequences from cyanobacterial mats of Antarctic (Byers Peninsula) origin obtained by clone library

Versions

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Droits

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L’éditeur et détenteur des droits de cette ressource est SCAR - Microbial Antarctic Resource System. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.

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Mots-clé

Metadata

Contacts

Julia Kleinteich
  • Créateur
  • Personne De Contact
Researcher
University of Liège
Liège
Lieège
BE
Julien Cigar
  • Fournisseur Des Métadonnées
Daniel R Dietrich
  • Personne De Contact
Researcher
University of Konstanz
Konztanz
DE

Couverture géographique

Byers Peninsula, Livingston Island, Antarctica

Enveloppe géographique Sud Ouest [-62,7, -61,5], Nord Est [-62,5, -61]

Couverture taxonomique

All data provided are OTUs of the 16S rRNA gene which were identified via a comparison to online databases to family or genus level. For most OTUs identification to species level was not possible

Phylum Cyanobacteria (Cyanobacteria)

Couverture temporelle

Date de début / Date de fin 2009-02-01 / 2009-02-28

Données sur le projet

Pas de description disponible

Titre DI698/18-1 Dietrich
Financement Deutsche Forschungsgesellschaft DFG

Les personnes impliquées dans le projet:

Daniel R Dietrich
  • Chercheur Principal

Méthodes d'échantillonnage

Cyanobacterial samples were collected from five different sampling sites in the Antarctic Specially Protected Area (ASPA) No. 126 of Byers Peninsula, Livingston Island (62° 34' 35" to 62° 4' 35" S and 60° 54' 14" to 61° 13' 07" W), South Shetland Islands, Antarctic Peninsula, during an expedition in February 2009. Microbial mats were probed using a sterile spatula, sealed in sterile plastic bags or tubes and stored frozen (-20 °C) for DNA extraction.

Etendue de l'étude Samples were collected on Byers Peninsula (ASPA 126) in February 2009. This area is a summer snow- and ice-free area. Various microbial mats on wet soil, meltwater and seepages were sampled.
Contrôle qualité Data were published in a peer-reviewed journal DOI: 10.1038/NCLIMATE1418

Description des étapes de la méthode:

  1. After collection the samples were stored at -20°C until further processing. DNA was extracted from the samples in three replicates and combined. 16S rRNA genes were amplified using cyanobacteria specific primers (Saker et al. 2005). PCR Products were cloned using the TOPO TA Cloning Kit following the standard protocol. Two to three clones of each individual restriction fragment length polymorphism pattern were sequenced at GATC Biotech. All sequences were deposited with the GenBank database. Saker, M. L., Jungblut, A. D., Neilan, B. A., Rawn, D. F. K. & Vasconcelos, V. M. Detection of microcystin synthetase genes in health food supplements containing the freshwater cyanobacterium Aphanizomenon flos-aquae. Toxicon 46, 555?562 (2005).

Métadonnées additionnelles