Description
Cyanobacterial 16S rRNA gene sequences from cyanobacterial mats of Antarctic (Byers Peninsula) origin obtained by clone library
Versions
Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.
Droits
Les chercheurs doivent respecter la déclaration de droits suivante:
L’éditeur et détenteur des droits de cette ressource est SCAR - Microbial Antarctic Resource System. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.
Enregistrement GBIF
Cette ressource n'a pas été enregistrée sur le portail GBIF
Mots-clé
Metadata
Contacts
- Créateur ●
- Personne De Contact
- Researcher
- Fournisseur Des Métadonnées
- Personne De Contact
- Researcher
Couverture géographique
Byers Peninsula, Livingston Island, Antarctica
Enveloppe géographique | Sud Ouest [-62,7, -61,5], Nord Est [-62,5, -61] |
---|
Couverture taxonomique
All data provided are OTUs of the 16S rRNA gene which were identified via a comparison to online databases to family or genus level. For most OTUs identification to species level was not possible
Phylum | Cyanobacteria (Cyanobacteria) |
---|
Couverture temporelle
Date de début / Date de fin | 2009-02-01 / 2009-02-28 |
---|
Données sur le projet
Pas de description disponible
Titre | DI698/18-1 Dietrich |
---|---|
Financement | Deutsche Forschungsgesellschaft DFG |
Les personnes impliquées dans le projet:
- Chercheur Principal
Méthodes d'échantillonnage
Cyanobacterial samples were collected from five different sampling sites in the Antarctic Specially Protected Area (ASPA) No. 126 of Byers Peninsula, Livingston Island (62° 34' 35" to 62° 4' 35" S and 60° 54' 14" to 61° 13' 07" W), South Shetland Islands, Antarctic Peninsula, during an expedition in February 2009. Microbial mats were probed using a sterile spatula, sealed in sterile plastic bags or tubes and stored frozen (-20 °C) for DNA extraction.
Etendue de l'étude | Samples were collected on Byers Peninsula (ASPA 126) in February 2009. This area is a summer snow- and ice-free area. Various microbial mats on wet soil, meltwater and seepages were sampled. |
---|---|
Contrôle qualité | Data were published in a peer-reviewed journal DOI: 10.1038/NCLIMATE1418 |
Description des étapes de la méthode:
- After collection the samples were stored at -20°C until further processing. DNA was extracted from the samples in three replicates and combined. 16S rRNA genes were amplified using cyanobacteria specific primers (Saker et al. 2005). PCR Products were cloned using the TOPO TA Cloning Kit following the standard protocol. Two to three clones of each individual restriction fragment length polymorphism pattern were sequenced at GATC Biotech. All sequences were deposited with the GenBank database. Saker, M. L., Jungblut, A. D., Neilan, B. A., Rawn, D. F. K. & Vasconcelos, V. M. Detection of microcystin synthetase genes in health food supplements containing the freshwater cyanobacterium Aphanizomenon flos-aquae. Toxicon 46, 555?562 (2005).
Métadonnées additionnelles
Identifiants alternatifs | https://ipt.biodiversity.aq/resource?r=cyano_16s |
---|