Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica
This dataset describes heterotrophic bacteria that were isolated from five aquatic microbial mat samples from different locations in continental Antarctica and the Antarctic Peninsula.
Peeters K (2020): Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Samplingevent. https://ipt.biodiversity.aq/resource?r=heterotrophic_bacterial_isolates_in_antarctica_microbial_mats&v=1.0
此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
Different water bodies (lakes) on the Antarctic continent: East Antarctica, the Trans-Antarctic Mountains, and the Antarctic Peninsula
|界定座標範圍||緯度南界 經度西界 [-82.45, -67.45], 緯度北界 經度東界 [-67.7, 39.8]|
Heterotrophic Bacteria (504 strains) isolated from frozen microbial mat samples using culturing techniques.
The AMBIO project aimed to extend the baseline information of microbial diversity in aquatic habitats of terrestrial Antarctic environments.
|計畫名稱||Antarctic Microbial Biodiversity (AMBIO)|
|研究區域描述||the Antarctic continent.|
Microbial mat samples were collected aseptically using a sterile spatula, and were kept frozen continuously after collection until processing in the laboratory.
|研究範圍||Bacterial strain isolated from five aquatic microbial mat samples from different locations in continental Antarctica and the Antarctic Peninsula, sampled between 2003 and 2007.|
- One gram of sample was aseptically weighed and homogenized in 9 ml sterile cold (4°C) physiological saline (0.86% NaCl) using a vortex. Tenfold dilution series (kept at 4°C) were plated on four different media (Marine agar 2216 (MA) (BD DifcoTM), R2A (BD DifcoTM), ten times diluted R2A (R2A/10), and PYGV (Pepton-Yeast-Glucose-Vitamin) medium (DSMZ medium 621)) and incubated at 20°C, 15°C and 4°C. R2A (Difco) contains pyruvate, starch and dextrose as C sources and yeast extract, peptone and casaminoacids as N and C sources and PYGV (DSMZ medium 621) contains peptone, yeast extract and glucose as C and/or N sources and additional vitamins and minerals. Both are considered oligotrophic media because the amounts of these components are at least two to ten times lower than in more general media such as nutrient broth. In addition to regular physiological saline (PS) dilution series, sea water (SW) dilutions were used for the LA3 and WO10 samples which originated from lakes close to the ocean and had elevated conductivity values.
- K. Peeters, E. Verleyen, D. A. Hodgson, P. Convey, D. Ertz , W. Vyverman, A. Willems (2012). Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica. Polar Biol (2012) 35:543‐554. DOI 10.1007/s00300‐011‐1100‐4